Expression cloning of a dust mite cysteine proteinase, Der p1, a major allergen associated with asthma and hypersensitivity reactions

Abstract

Expression cloning of a dust mite cysteine proteinase, Drr pl. a major allergen associated with asthma and hypersensitivity reactionsThe house dust mite (HDM), Dermarophagoides pteronyssinus, is a major source of allergens, implicated in diseases such as asthma and atopic dermatitis. About 75% of

A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines

“…To our knowledge, aggregated recombinant ProDer p 1 obtained by heat denaturation constitutes the only full-length Der p 1 variant showing drastically reduced IgE reactivity. 18 Attempts to express mature Der p 1 in E coli have been unsuccessful 24,25 probably because of the absence of the prosequence that plays the role for chaperonin. 26 Recombinant ProDer f 1, the ProDer p 1 homolog from Dermatophagoides farinae, was produced in bacteria as inclusion bodies and could be renatured after denaturation/refolding steps.…”

On the potential significance of the enzymatic activity of mite allergens to immunogenicity. Clues to structure and function revealed by molecular characterization

“…As indicated earlier, Der p 1 is polymorphic. A number of naturally occurring variants have been identified; they include A 3 G, H 50 Y, H 72 S, E 81 K, A 124 V, S 136 T and E 215 Q (17,54), The mutations occur mainly on surface-exposed residues and, on the basis of the model, they are unlikely to account for functional deficits (unpublished observations).…”

High‐level expression of recombinant house dust mite allergen Der p 1 in Pichia pastoris

“…The first attempts at recombinant Der p 1 productions were shown to be unsuccessful in S. cerevisiae and E. coli [8–10]. Recombinant Der p 1 was poorly expressed, mainly under an insoluble form, and displayed weaker IgE‐binding reactivity than the native allergen.…”