Expression changes of gelatinases in human osteoarthritic knees and arthroscopic debridement

Hsieh, Yih‐Shou; Yang, Shun‐Fa; Chu, Shu‐Chen; Chen, Pei‐Ni; Chou, Ming-Chih; Hsu, Ming-Cheng; Lu, Ko-Hsiu

doi:10.1016/j.arthro.2004.03.010

Cited by 34 publications

(33 citation statements)

References 29 publications

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“…There is strong circumstantial evidence that MMP-2 participates in the turnover of normal cartilage matrix, whereas MMP-9 and some MMP-2 facilitate the progressive destruction of cartilage matrix in OA. 24) Our results indicate that HPE inhibits collagen degradation through inhibition of MMP-2 and -9 in the early stage of OA.…”

Section: Discussionmentioning

confidence: 69%

Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis

Kim

Park

et al. 2010

Biological & Pharmaceutical Bulletin

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This study investigated the effect of human placenta extract (HPE) on cartilage degradation in vitro MG-63 cells, articular cartilage explants, and in vivo monoiodoacetate (MIA)-induced osteoarthritis (OA). Matrix metalloproteinase (MMP)-2 activity was measured in HPE-treated osteoblastic MG-63 cells. Articular cartilage explants in rabbit were cultured, and the degree of proteoglycan (PG) degradation was assessed by measuring the amount of glycosaminoglycan (GAG) released into the culture medium. Experimental osteoarthritis was induced by intra-articular injection of 3 mg MIA in rats. Beginning 14 d post-MIA injection, HPE was administered intra-articularly once a day for 14 d. The knee joints were assessed by roentgenography, histology, and gelatinase activity. HPE inhibited PG degradation in articular cartilage explants. HPE significantly reduced deformity of knee joints and suppressed the histological change in MIA-induced OA. HPE inhibited MMP-2 activity in MG-63 cells. MMP-2 and -9 activities were also reduced in the cartilages of HPE-treated knee joints. Our results indicate that HPE has therapeutic effects on OA by protecting cartilage.

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Section: Discussionmentioning

confidence: 69%

Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis

Kim

Park

et al. 2010

Biological & Pharmaceutical Bulletin

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“…Specimens of over 350 mg from diseased cartilage, torn menisci and hypertrophic synovia, that were all small fragments after arthroscopic débridement from 6 patients, including two men (with an age of 60 and 66) and four women (aged 53, 62, 64 and 70), were obtained from the patients with primary early OA knees (fulfilled the American College of Rheumatology criteria and corresponded to grade II-III in the Kellgren and Lawrence classification system) by the same author at our hospital [2]. The interval between the onset of severe pain and the arthroscopic procedure of these five patients ranged from 4 to 18 months (with a median of 6 months).…”

Section: Chondral Meniscal and Synovial Culturesmentioning

confidence: 99%

“…Each diseased tissue from one patient was divided into seven groups (one control and six study groups) and weighted equally 50 mg, transferred into 24-well culture dishes respectively and then incubated at 37°C under a humidified atmosphere of 5% CO 2 in DMEM supplemented with 2% penicillin-streptomycin (10,000 U/mL) and 10 mg/mL streptomycin. The tissue culture system employed has been used previously [2,9].…”

Section: Chondral Meniscal and Synovial Culturesmentioning

confidence: 99%

“…An optimized LDH test (Promega Corp., Madison, WI, USA) was used to quantify LDH activity in the conditioned medium of chondral, meniscal and synovial cultures after treatment with or without different NSAIDs for 3, 24, 48 and 96 h. Like extraction of RNA from the tissue block in our previous study [2], each tissue of cartilage, menisci and synovia was homogenized in 1 mL of phosphatebuffered saline to determine total LDH activity as positive control [22,25].…”

Section: Measurement Of Ldh Activitymentioning

confidence: 99%

“…The degradation of OA cartilage matrix has been shown to be related to the excess synthesis of a large number of proteases and, more particularly, to that of matrix metalloproteinases (MMPs). Gelatinases (type IV collagenases) can be divided into gelatinase-A (MMP-2) and gelatinase-B (MMP-9) and their expressions increase in pathologic chondral, meniscal and synovial lesions of OA, which is likewise believed to play an important role in OA pathophysiology [2]. In fact, increased expression of gelatinases in arthritic effusions easily reflected the inflammatory condition of joints [3][4][5][6], and nonsteroidal antiinflammatory drugs (NSAIDs) may down-regulate the MMPs in synovial fluids of gouty arthritis [3].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of nonsteroidal anti-inflammatory drugs on the expression of urokinase plasminogen activator and inhibitor and gelatinases in the early osteoarthritic knee of humans

Yang¹,

Hsieh²,

Ko³

et al. 2008

Clinical Biochemistry

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Effects of different molecular weight hyaluronan products on the expression of urokinase plasminogen activator and inhibitor and gelatinases during the early stage of osteoarthritis

Hsieh

Yang

et al. 2007

Journal Orthopaedic Research

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Hyaluronan or hyaluronic acid (HA) has been used to treat osteoarthritic knees for more than 30 years. Here, we tested the hypothesis that HA with high molecular weight (MW) would have greater effects than HA with low MW on the expression of the plasminogen activator (PA)/ plasmin system and gelatinases [matrix metalloproteinase (MMP)-2 and MMP-9] during early development of osteoarthritis (OA). We compared the levels of MMP-2, MMP-9, urokinase-type PA (u-PA), and PA inhibitor-1 (PAI-1) in a series of chondral, meniscal, and synovial cultures of early OA after treatment with or without three different MW HA products (Hyalgan and Artz with low MW, and Synvisc with high MW). Gelatin zymography revealed that three different HA products could decrease the secretion of MMP-2 in all tissue cultures and MMP-9 in meniscal and synovial cultures time-dependently. Enzyme-linked immunosorbent assay showed that Artz and Synvisc had significant inhibition on u-PA and PAI-1 levels after 24 h, but Hyalgan did at 96 h. Compared with Hyalgan and Artz, Synvisc provided the greatest ability to inhibit MMP-2, MMP-9, u-PA, and PAI-1 expression. Our studies clearly demonstrate that the therapeutic effects of using HA to treat early OA may be partially dependant on downregulation of the PA/plasmin system and gelatinases expression, which delay the structural progression of the disease. HA with high MW might have a greater ability than that with low MW to offer effective protection for articular cartilage. ß

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Expression changes of gelatinases in human osteoarthritic knees and arthroscopic debridement

Cited by 34 publications

References 29 publications

Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis

Protective Effects of Human Placenta Extract on Cartilage Degradation in Experimental Osteoarthritis

Effects of nonsteroidal anti-inflammatory drugs on the expression of urokinase plasminogen activator and inhibitor and gelatinases in the early osteoarthritic knee of humans

Effects of different molecular weight hyaluronan products on the expression of urokinase plasminogen activator and inhibitor and gelatinases during the early stage of osteoarthritis

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