Expression and silencing of Selenoprotein M (SelM) from the white shrimp Litopenaeus vannamei: Effect on peroxidase activity and hydrogen peroxide concentration in gills and hepatopancreas

García-Triana, Antonio; Gómez‐Jiménez, Silvia; Peregrino‐Uriarte, Alma B.; López-Zavala, Alonso A.; González‐Aguilar, Gustavo A.; Sotelo‐Mundo, Rogerio R.; Valenzuela‐Soto, Elisa M.; Yépiz-Plascencia, Glória

doi:10.1016/j.cbpa.2009.10.037

Cited by 23 publications

(5 citation statements)

References 27 publications

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“…Studies showed that the generation of ROS was inherent in the hepatopancreas of white shrimp ( Litopenaeus vannamei ) (García‐Triana et al . ) and intestinal epithelial cells of mouse (Datta et al . ).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary phenylalanine on growth, digestive and brush border enzyme activities and antioxidant capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idella)

Feng

Liu

et al. 2015

Aquacult Nutr

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This study investigated the effects of phenylalanine on growth, digestive and absorptive ability and antioxidant status of young grass carp (Ctenopharyngodon idella). Young grass carp were fed diets containing 3.4 (basal diet), 6.1, 9.1, 11.5, 14.0 and 16.8 g phenylalanine kg À1 diet with a fixed of 10.7 g tyrosine kg À1 diet for 8 weeks. Percent weight gain (PWG), feed efficiency and feed intake of fish were the lowest in fish fed the basal diet (P < 0.05). Trypsin, lipase and amylase activities in the hepatopancreas, and antioxidants including glutathione contents and glutathione reducase activities in the hepatopancreas and intestine were all the highest in fish fed 11.5 g phenylalanine kg À1 diet (P < 0.05). Trypsin, chymotrypsin and amylase activities in whole intestine, and creatine kinase, Na + , K + -ATPase and alkaline phosphatase activities in the proximal intestine, and superoxide dismutase activities in the hepatopancreas and intestine were all the highest when phenylalanine at level of 9.1 g kg À1 diet (P < 0.05). In conclusion, phenylalanine improved growth, digestive and absorptive ability, and antioxidant capacity of young grass carp. The phenylalanine requirement of young grass carp (256-629 g) based on PWG was 10.4 g kg À1 diet or 3.44 g 100 g À1 protein.

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Section: Introductionmentioning

confidence: 99%

Effects of dietary phenylalanine on growth, digestive and brush border enzyme activities and antioxidant capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idella)

Feng

Liu

et al. 2015

Aquacult Nutr

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“…Similarly, no PcGPx expression was detected in brain, ganglia and muscle from the red swamp P. clarkii [ 9 ] and also, no MeGPx expression was detected in brain and ganglia of the shrimp M. ensis [ 6 ]. On the other hand, transcripts of a peroxidase selenoprotein M ( SelM ) were reported in L. vannamei Pl and muscle [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

cDNA Characterization and Expression of Selenium-Dependent CqGPx3 Isoforms in the Crayfish Cherax quadricarinatus under High Temperature and Hypoxia

Hernández-Aguirre

Fuentes-Sidas

Rivera-Rangel

et al. 2022

Genes

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Glutathione peroxidase 3 (GPx3) is the only extracellular selenoprotein (Sel) that enzymatically reduces H2O2 to H2O and O2. Two GPx3 (CqGPx3) cDNAs were characterized from crayfish Cherax quadricarinatus. The nerve cord CqGPx3a isoform encodes for a preprotein containing an N-terminal signal peptide of 32 amino acid residues, with the mature Sel region of 192 residues and a dispensable phosphorylation domain of 36 residues. In contrast, the pereiopods CqGPx3b codes for a precursor protein with 19 residues in the N-terminal signal peptide, then the mature 184 amino acid residues protein and finally a Pro-rich peptide of 42 residues. CqGPx3 are expressed in cerebral ganglia, pereiopods and nerve cord. CqGPx3a is expressed mainly in cerebral ganglia, antennulae and nerve cord, while CqGPx3b was detected mainly in pereiopods. CqGPx3a expression increases with high temperature and hypoxia; meanwhile, CqGPx3b is not affected. We report the presence and differential expression of GPx3 isoforms in crustacean tissues in normal conditions and under stress for high temperature and hypoxia. The two isoforms are tissue specific and condition specific, which could indicate an important role of CqGPx3a in the central nervous system and CqGPx3b in exposed tissues, both involved in different responses to environmental stressors.

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“…The increased LvMT mRNA expression along with the rapid increased expression or activity of catalase (Trasviña-Arenas et al, 2013), total SOD and GPx in response to hypoxia (Parrilla-Taylor and Zenteno-Savín, 2011), and also the increased cMnSOD transcript levels and SOD activity in response to reoxygenation (García-Triana et al, 2010) is in line with the "preparation for oxidative stress" hypothesis proposed by Hermes-Lima et al (1998), which states that in organisms evolutionary adapted to transitions between normal and extreme oxygen concentrations, an exposure to extreme situations induces the adaptive response that helps them survive at recovery by attenuating the effects of increased ROS formation during hypoxia/reoxygenation. MT antioxidant activity might indeed contribute to protect L. vannamei from oxidative damage, since it can remove O 2 $ and$OH simultaneously and can react with OH about 10,000 times faster than SOD (Mao et al, 2012).…”

Section: Expression Profile Of Mt Under Hypoxia Challengementioning

confidence: 96%

The metallothionein gene from the white shrimp Litopenaeus vannamei : Characterization and expression in response to hypoxia

Félix-Portillo

Martínez-Quintana

Peregrino‐Uriarte

et al. 2014

Marine Environmental Research

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Expression and silencing of Selenoprotein M (SelM) from the white shrimp Litopenaeus vannamei: Effect on peroxidase activity and hydrogen peroxide concentration in gills and hepatopancreas

Cited by 23 publications

References 27 publications

Effects of dietary phenylalanine on growth, digestive and brush border enzyme activities and antioxidant capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idella)

Effects of dietary phenylalanine on growth, digestive and brush border enzyme activities and antioxidant capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idella)

cDNA Characterization and Expression of Selenium-Dependent CqGPx3 Isoforms in the Crayfish Cherax quadricarinatus under High Temperature and Hypoxia

The metallothionein gene from the white shrimp Litopenaeus vannamei : Characterization and expression in response to hypoxia

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