Expression and Significances of Contactin-1 in Human Gastric Cancer

Abstract

Background. This study aimed at determining the relationship between vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3), and contactin-1 (CNTN-1) expression in gastric cancer (GC). Methods. The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot. And 105 cases were immunohistochemically examined for VEGF-C, VEGFR-3, and CNTN-1 expressions. Assessment of lymphatic vessel density (LVD) was also performed by D2-40 immunostaining. Then we analyzed the relationships between VEGF-C, VEGFR-3, and CNTN-1, as well as their correlations with clinicopathologic features, LVD, and survival time. Results. The positivity rate of VEGF-C, VEGFR-3, and CNTN-1 in primary tumor was 56.19%, 64.76%, and 58.09%. The expression of CNTN-1 significantly correlated with VEGF-C (P < 0.001) and VEGFR-3 (P < 0.001). All of them were closely related to TNM stage, lymphatic invasion, and lymph node involvement (P < 0.05). LVD was significantly correlated with VEGF-C (P = 0.001), VEGFR-3 (P = 0.011), and CNTN-1 expression (P < 0.001). VEGF-C, VEGFR-3, and CNTN-1 expression significantly associated with poorer prognosis (P < 0.001, P = 0.034, P = 0.012, resp.). Conclusion. CNTN-1 associated with VEGF-C and VEGFR-3 expression in GC. All of them correlated with lymphatic metastasis, which might play an important role in the lymphatic invasion via lymphangiogenesis pathway in GC.

Increased sensitivity of human lung adenocarcinoma cells to cisplatin associated with downregulated contactin-1

“…CNTN-1, a glycosyl phosphatidylinositol NCAM, was found to play important roles not only in nervous system development [17,18,30] but also in the invasion and metastasis of lung adenocarcinoma [19,31,32] and other tumours [20,22,23]. This study was performed to investigate whether CNTN-1 is associated with MDR in lung adenocarcinoma, and the findings revealed that CNTN-1 plays an important role in MDR of lung adenocarcinoma besides invasion and metastasis.…”

“…Subsequent study revealed that CNTN-1 was associated with invasive ability and to inversely correlate with the prognosis of lung adenocarcinoma patients, and it was proposed to function in the invasion and metastasis of lung adenocarcinoma cells via RhoA-mediated mechanisms [19]. Studies in oesophageal squamous cell carcinoma [20,21], oral squamous cell carcinoma [22], and gastric cancer [23] further confirmed that CNTN-1 plays a critical role in the development, progression, and pathogenesis of these tumours.…”

CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

“…Recently, CNTN-1 was demonstrated to be able to promote invasion, metastasis and proliferation in prostate cancer [43], thyroid cancer [44], gastric cancer [35, 45-46], lung cancer [32-33], esophageal squamous cancer [30, 47], and oral squamous cancer [31]. Additionally, in our previous studies, CNTN-1 was found to enhance the metastasis, invasion, and chemoresistance of lung cancer cells [36].…”

Association of MLL3 expression with prognosis in gastric cancer

“…Recent studies have focused on identifying molecular markers and developing related active treatment methods for gastric cancer. Efforts have been made to identify biomarkers for predicting survival or recurrence in gastric cancer (Wang et al, 2013;Sezer et al, 2013;Yu et al, 2013).…”