Expression and significance of Twist, estrogen receptor, and E-cadherin in human breast cancer cells and tissues

Tan, Ruizhi; Wang, Li; Song, Jie; Li, Jianchun; He, Tao

doi:10.4103/jcrt.jcrt_1396_16

Cited by 9 publications

(3 citation statements)

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“…In addition, we found that G. lucidum extract inhibited cell migration, as determined by a wound-healing assay ( Figure 5 A–D), and this effect was associated with the upregulation of E-cadherin, which plays an important role in the process of cell adhesion and is downregulated in many tumors [ 30 ]. Consistent with our results, an earlier study reported that G. lucidum whole extract significantly inhibits breast cancer cell invasion by upregulating E-cadherin [ 31 ]. Taken together, these findings suggest that G. lucidum extract may inhibit the migration of glioblastoma cells, mainly by upregulating E-cadherin expression ( Figure 5 E,F).…”

Section: Discussionsupporting

confidence: 93%

Water-Extracted Ganoderma lucidum Induces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

et al. 2020

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Glioblastoma is one of the most common and most aggressive brain cancers. The current treatment is mainly surgery, chemotherapy, and radiation therapy, but the results are not satisfactory. Ganoderma lucidum (G. lucidum), also called “Lingzhi”, is a medicinal mushroom that has been used as a therapeutic agent for the treatment of numerous diseases, including cancer. However, whether it is effective for treating cancer is still unclear. In the present study, the anti-tumor effect of a water extract of G. lucidum was investigated using brain tumor cells. We used an analysis of cell viability, flow cytometry, the IncuCyte live-cell analysis system, and Western blotting to study its effects. The water extract from G. lucidum inhibited cell proliferation in a dose- and time-dependent manner, and it induced mitochondria-mediated apoptosis and cell cycle arrest at S phase via the cyclin-CDK2 pathway in human brain tumor cells. In addition, the G. lucidum extract significantly inhibited cell migration and mesenchymal marker expression based on the IncuCyte live-cell assay and qRT-PCR analysis. In summary, these anti-tumor effects in brain tumor cells suggest that G. lucidum may be useful for treating brain tumors.

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Section: Discussionsupporting

confidence: 93%

Water-Extracted Ganoderma lucidum Induces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

et al. 2020

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“…The phenomenon of EMT manifests itself by the coordinated recruitment of different genes and signaling pathways in each of the molecular subtypes of breast cancer. Even though several studies have shown that overexpression of Snail, Twist, and N-cadherin leads to aggressive cancer progression [18,[63][64][65], our findings demonstrated that the levels of protein expression of the transcription factors Snail and Twist failed to hold significant association with the studied clinical-pathological criteria. N-cadherin, a recognized marker of mesenchymal differentiation and strongly linked to cell mobility, was correlated with the histological grade and molecular subtype.…”

Section: Discussioncontrasting

confidence: 82%

Impact of Epithelial–Mesenchymal Transition on the Immune Landscape in Breast Cancer

Khadri

Issac

Gaboury

2021

Cancers

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The impact of epithelial–mesenchymal transition (EMT) signature on the immune infiltrate present in the breast cancer tumor microenvironment (TME) is still poorly understood. Since there is mounting interest in the use of immunotherapy for the treatment of subsets of breast cancer patients, it is of major importance to understand the fundamental tumor characteristics which dictate the inter-tumor heterogeneity in immune landscapes. We aimed to assess the impact of EMT-related markers on the nature and magnitude of the inflammatory infiltrate present in breast cancer TME and their association with the clinicopathological parameters. Tissue microarrays were constructed from 144 formalin-fixed paraffin-embedded invasive breast cancer tumor samples. The protein expression patterns of Snail, Twist, ZEB1, N-cadherin, Vimentin, GRHL2, E-cadherin, and EpCAM were examined by immunohistochemistry (IHC). The inflammatory infiltrate in the TME was assessed semi-quantitatively on hematoxylin and eosin (H&E)-stained whole sections and was characterized using IHC. The inflammatory infiltrate was more intense in poorly differentiated carcinomas and triple-negative carcinomas in which the expression of E-cadherin and GRHL2 was reduced, while EpCAM was overexpressed. Most EMT-related markers correlated with plasma cell infiltration of the TME. Taken together, our findings reveal that the EMT signature might impact the immune response in the TME.

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“…In doing so, EMT is reversed while VM is inhibited (60). In breast cancer tumors, overexpression of TWIST is associated with a lower expression of epithelial factors such as E-cadherin (61,62). Increased TWIST levels also correlate with more advanced stage tumor and are more common in TNBC tumors and HER2+ (63).…”

Section: Relationship Between Cscs Vm and The Epithelial-mesenchymalmentioning

confidence: 99%

An Overview of Vasculogenic Mimicry in Breast Cancer

et al. 2020

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Vasculogenic mimicry (VM) is the formation of vascular channels lacking endothelial cells. These channels are lined by tumor cells with cancer stem cell features, positive for periodic acid-Schiff, and negative for CD31 staining. The term VM was introduced by Maniotis et al. (1), who reported this phenomenon in highly aggressive uveal melanomas; since then, VM has been associated with poor prognosis, tumor aggressiveness, metastasis, and drug resistance in several tumors, including breast cancer. It is proposed that VM and angiogenesis (the de novo formation of blood vessels from the established vasculature by endothelial cells, which is observed in several tumors) rely on some common mechanisms. Furthermore, it is also suggested that VM could constitute a means to circumvent anti-angiogenic treatment in cancer. Therefore, it is important to determinant the factors that dictate the onset of VM. In this review, we describe the current understanding of VM formation in breast cancer, including specific signaling pathways, and cancer stem cells. In addition, we discuss the clinical significance of VM in prognosis and new opportunities of VM as a target for breast cancer therapy.

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Expression and significance of Twist, estrogen receptor, and E-cadherin in human breast cancer cells and tissues

Abstract: Taken together, these findings demonstrated that Twist was upregulated in high invasion and metastasis cell lines as well as IDC tissues companioned with downregulated expression of E-cadherin and ER, which provides important clues for the deeper study of breast cancer.

Cited by 9 publications

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Water-Extracted Ganoderma lucidum Induces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

Water-Extracted Ganoderma lucidum Induces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

Impact of Epithelial–Mesenchymal Transition on the Immune Landscape in Breast Cancer

An Overview of Vasculogenic Mimicry in Breast Cancer

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