Expression and significance of Parkinson disease protein 7 in placental, serum and umbilical cord blood in preeclampsia

Yang, Tingting; Yan, Jianying; Han, Qing; Zhang, Qinjian; Liao, Qiuping

doi:10.5603/gp.a2020.0131

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrophobic L187 residue can also alleviate the ferroptosis effect of erastin on DJ-1−/− mouse embryonic fibroblasts 27 . In a previous study, we found that the expression levels of DJ-1 mRNA and protein in the serum and placenta of patients with PE were significantly increased 28 . We also found that the up-regulated levels of Nrf2/GPX4 in the placenta of patients with severe PE enhanced the invasive ability of trophoblasts 29 .…”

Section: Discussionmentioning

confidence: 86%

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia

Liao

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Ferroptosis is a newly discovered mode of cell death that involves disorders in iron metabolism and the accumulation of reactive oxygen species (ROS) in the plasma membrane. Preeclampsia (PE) is a gestational idiopathic disease that is characterized by hypertension and albuminuria, begins after 20 weeks of pregnancy. DJ-1 is a prerequisite for activating and stabilizing Nrf2 to allow translocation to the nucleus to carry out further functions. Detecting the expression levels of DJ-1, the Nrf2/GPX4 signaling pathway and ferroptosis markers in placental tissues of pregnant women with and without PE. Analyzing the effects of the ferroptosis inducer (RSL3) and the inhibitor (Fer-1) on the mortality rate of BeWo cells and DJ-1+/+, DJ-1−/− BeWo cells. Ferroptosis markers (MDA concentration and morphology of trophoblast cells) and DJ-1 and its downstream the Nrf2/GPX4 signaling pathway increased significantly in PE pathological state. The expression levels of DJ-1 protein in the control group and the PE group were positively correlated with the expression levels of Nrf2/GPX4 signaling pathway protein, and negatively correlated with the MDA concentration. BeWo cells were sensitive to the ferroptosis inducer (RSL3) and the inhibitor (Fer-1). The high expression levels of DJ-1 in BeWo cells can resist ferroptosis by regulating the Nrf2/GPX4 signaling pathway. Ferroptosis is involved in the pathogenesis of PE. DJ-1 can mediate the trophoblast cells ferroptosis and play a protective role in the pathogenesis of preeclampsia by regulating the Nrf2/GPX4 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 86%

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia

Liao

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The VHL binding protein DJ1 regulates the function of HIF-1α in both cancer and neuronal cells through binding VHL and limiting HIF-1α degradation [ 27 , 28 ]. DJ1 was reported to be increased in the placentae of PE women [ 42 , 43 ]. However, although we detected DJ1 in placental tissue from our PE and IUGR cohorts, there was no evidence of increased expression relative to controls.…”

Section: Discussionmentioning

confidence: 99%

“…However, although we detected DJ1 in placental tissue from our PE and IUGR cohorts, there was no evidence of increased expression relative to controls. While we compared levels of DJ1 in early gestation samples from normal and PE pregnancies, Yang et al [ 43 ] compared DJ1 expression in early-onset preeclamptic placentae with late control placental tissues. The use of SPT controls in our study and term tissues by Yang and colleagues may explain these differences as it is currently unknown whether expression of DJ1 varies with gestational age similar to HIF-1α.…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of Oxygen Sensing/Response Pathways in Pregnancies Complicated by Idiopathic Intrauterine Growth Restriction and Early-Onset Preeclampsia

McCracken

Seeho

Carrodus

et al. 2022

IJMS

View full text Add to dashboard Cite

Preeclampsia (PE) and intrauterine growth restriction (IUGR) are the leading causes of maternal and fetal morbidity/mortality. The central deficit in both conditions is impaired placentation due to poor trophoblast invasion, resulting in a hypoxic milieu in which oxidative stress contributes to the pathology. We examine the factors driving the hypoxic response in severely preterm PE (n = 19) and IUGR (n = 16) placentae compared to the spontaneous preterm (SPT) controls (n = 13) using immunoblotting, RT-PCR, immunohistochemistry, proximity ligation assays, and Co-IP. Both hypoxia-inducible factor (HIF)-1a and HIF-2a are increased at the protein level and functional in pathological placentae, as target genes prolyl hydroxylase domain (PHD)2, PHD3, and soluble fms-like tyrosine kinase-1 (sFlt-1) are increased. Accumulation of HIF-a-subunits occurs in the presence of accessory molecules required for their degradation (PHD1, PHD2, and PHD3 and the E3 ligase von Hippel–Lindau (VHL)), which were equally expressed or elevated in the placental lysates of PE and IUGR. However, complex formation between VHL and HIF-a-subunits is defective. This is associated with enhanced VHL/DJ1 complex formation in both PE and IUGR. In conclusion, we establish a significant mechanism driving the maladaptive responses to hypoxia in the placentae from severe PE and IUGR, which is central to the pathogenesis of both diseases.

show abstract

The association between increased maternal PARK7 (DJ-1) levels and the occurrence of preterm premature rupture of membranes - A randomized prospective study

Turhan¹,

Tatar²

2021

tjod

View full text Add to dashboard Cite

Expression and significance of Parkinson disease protein 7 in placental, serum and umbilical cord blood in preeclampsia

Cited by 4 publications

References 0 publications

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia

Dysregulation of Oxygen Sensing/Response Pathways in Pregnancies Complicated by Idiopathic Intrauterine Growth Restriction and Early-Onset Preeclampsia

The association between increased maternal PARK7 (DJ-1) levels and the occurrence of preterm premature rupture of membranes - A randomized prospective study

Contact Info

Product

Resources

About