Expression and significance of miR-654-5p and miR-376b-3p in patients with colon cancer

Li, Ping; Cai, Jia-Xun; Han, Fei; Wang, Jie; Zhou, Jiajie; Shen, Kaiwen; Wang, Liuhua

doi:10.4251/wjgo.v12.i4.492

Cited by 13 publications

(8 citation statements)

References 23 publications

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“…In a mouse study, overexpression of mir-1193-5p was found to be able to increase the differentiation tendency of oligodendrocyte progenitor cells (46). Similarly, previous studies have found that mir-654 is significantly related to the development of a variety of malignant tumors, including lung cancer, rectal cancer, esophageal cancer and colon cancer (47,48). Since we only detected the above two miRNAs in SP42-2 individuals, it is not possible to determine their relationship with the photoperiod and adrenal gland, so we will explore further.…”

Section: Discussionmentioning

confidence: 77%

Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)

Liu

et al. 2022

Front. Vet. Sci.

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In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes GRHL2, CENPF, FGF16 and SLC25A30 that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., SLC25A30 coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep.

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Section: Discussionmentioning

confidence: 77%

Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)

Liu

et al. 2022

Front. Vet. Sci.

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“…Our results supported RGS1 as a tumor-promoting gene in osteosarcoma. MiR-376b-3p is a tumor-suppressive gene that is down-regulated in multiple tumors (21)(22)(23). For example, miR-376b-3p has been shown to be more significantly differentially expressed in the tumors of patients with primary resistance of renal cell carcinoma patients (24).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

Zhang¹,

Meng²,

Ma³

et al. 2021

Ann Transl Med

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Background: To investigate the role of microRNA-376b-3p (miR-376b-3p) and regulator of G protein signaling 1 (RGS1) in the proliferation, metastasis, and apoptosis of osteosarcoma.Methods: Differentially expressed genes (DEGs) between tumor and normal tissues from GSE14359 and GSE33382 in the cancer genome atlas (TCGA) dataset were analyzed with GEO2R online. Similarly, differentially expressed miRNAs from GSE70367 were also analyzed with GEO2R. The interaction between the differentially expressed miRNAs and the shared distal metastasis-related DEGs from the two datasets were analyzed using miRWalk and Cytoscape. RGS1 and miR-376-3p were chosen to verify the prediction.RGS1 stably expressing and silencing cells were established based on the MG63 and U2OS cell lines. The targeting of RGS1 with miR-376b-3p was confirmed with Starbase prediction and luciferase reporter assay.Cell proliferation, metastasis, and apoptosis were characterized in vitro and in xenograft mice.Results: A total of 10 up-regulated and 8 down-regulated DEGs were characterized as shared metastasisrelated DEGs for GSE14359 and GSE33382. Among these DEGs, RGS1 was targeted with miR-376b-3p, a predicted down-regulated miRNA in GSE70367. High expression of RGS1 predicted proliferation, invasion, metastases, and poor prognosis in osteosarcoma. Overexpression of RGS1 promoted proliferation, invasion, mobility, and stemness in MG63 and U2OS cells, while silencing of RGS1 had the opposite effect in both cell lines. High expression of RGS1 promoted tumor growth in xenograft nude mice. RGS1 was targeted with miR-376b-3p; the addition of miR-376b-3p down-regulated RGS1, and suppressed cell proliferation, invasion, and metastasis. Meanwhile, sponging of miR-376b-3p had the opposite effect. The suppressive effects of miR-376b-3p could be abolished with RGS1, as cell proliferation, stemness, metastasis, and invasion were all promoted with RGS1 co-transfection in both cell lines.Conclusions: Our study indicated that RGS1 is a tumor-promoting gene in osteosarcoma, which could be inhibited with miR-376b-3p.

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“…miR-654-3p is aberrantly expressed in several types of human cancers ( 46 , 47 ). However, its expression status and roles in ESCC remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Long intergenic non‑coding RNA LINC01232 contributes to esophageal squamous cell carcinoma progression by sequestering microRNA‑654‑3p and consequently promoting hepatoma‑derived growth factor expression

et al. 2020

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Long intergenic non-coding RNA 01232 (LINC01232) was identified as a critical regulator of the development of pancreatic adenocarcinoma. The present study investigated the expression and regulatory roles of LINC01232 in esophageal squamous cell carcinoma (ESCC). The main aim of the present study was to elucidate the underlying mechanisms through which LINC01232 affects the malignancy of ESCC. Initially, LINC01232 expression in ESCC was analyzed using the TCGA and GTEx databases and was confirmed using reverse transcription-quantitative polymerase chain reaction. ESCC cell proliferation, apoptosis and migration and invasion were assessed using the Cell Counting kit-8 assay, flow cytometric analysis, and migration and invasion assays, respectively. ESCC tumor growth in vivo was examined using a xenograft mouse model. As shown by the results, a high LINC01232 expression was detected in ESCC tissues and cell lines. LINC01232 downregulation suppressed the proliferation, migration and invasion of ESCC cells, and promoted cell apoptosis in vitro . In addition, LINC01232 depletion restricted tumor growth in vivo . Mechanistically, LINC01232 was shown to function as an microRNA-654-3p (miR-654-3p) sponge in ESCC cells, and hepatoma-derived growth factor (HDGF) was identified as a direct target of miR-654-3p. LINC01232 could bind competitively to miR-654-3p and decrease its expression in ESCC cells, thereby promoting HDGF expression. Rescue experiments reconfirmed that the effects of LINC01232 deficiency in ESCC cells were restored by increasing the output of the miR-654-3p/HDGF axis. On the whole, the present study demonstrates that LINC01232 plays a tumor-promoting role during the progression of ESCC by regulating the miR-654-3p/HDGF axis. The LINC01232/miR-654-3p/HDGF pathway may thus provide a novel theoretical basis for the management of ESCC.

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Expression and significance of miR-654-5p and miR-376b-3p in patients with colon cancer

Cited by 13 publications

References 23 publications

Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)

Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

Long intergenic non‑coding RNA LINC01232 contributes to esophageal squamous cell carcinoma progression by sequestering microRNA‑654‑3p and consequently promoting hepatoma‑derived growth factor expression

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