Expression and Sequence Analysis of CYP86B1 in Blackberry (Rubus Spp.)

Abstract

Abstract.To understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene CYP86B1 was cloned from blackberry. The open reading frame of this gene was 1692 bp, encoding 563 amino acid residues with a molecular weight of 64.5 kD and a theoretical isoelectric point of 7.31. Its amino acid sequence has the highest identity with strawberry. Real-time PCR results showed that the expression of CYP86B1 gene was significantly decreased with the accompanied by fruit ripening.