Expression and role of nuclear receptor-interacting protein 1 (NRIP1) in stomach adenocarcinoma

Abstract

Background: Nuclear receptor-interacting protein 1 (NRIP1), also named NR140, has been observed differentially express in multiple cancers, but the expression levels and the prognostic role of NRIP1 in stomach adenocarcinoma (STAD) remain unclear.Methods: We used the Gene Expression Profiling Interactive Analysis (GEPIA) to analyze the NRIP1 expression levels in STAD, subgroups analysis of expression of NRIP1 via the UALCAN dataset. Further, cBioPortal was used to investigate the aberration type, co-mutations status, and located mutation of NRIP1.Correlated genes, and kinases, microRNA (miRNA), and transcription factor (TF) targets were identified using LinkedOmics. The Kaplan-Meier (K-M) plotter was used to analyze the prognosis of NRIP1 and the significantly correlated genes in STAD. Then, the tumor immune estimation resource (Timer) was used to explore the relation between NRIP1 and the immune cell infiltration, and the role of immune cells in STAD.The Human Protein Atlas (HPA) was used to confirm the NRIP1 protein express in STAD stomach tissue and normal stomach tissue.Results: NRIP1 significantly overexpress in STAD, and the NRIP1 expression levels were impacted by clinical features. Overexpression of NRIP1 indicated the poor prognosis of STAD. Functional enrichment analysis showed the NRIP1 mainly enriched in immune response-regulating signaling pathway, cell-substrate adhesion, mRNA processing, and pathway in cancer. Overexpression USP25, SNYJ1 indicated the poor outcome of STAD, but the overexpression of BACH1 indicated protective biomarker. MIR-331 and MIR-132 have important role in STAD. Further, NRIP1 had a significant relation with immune infiltrates and other defined genes that significantly impact immune infiltrates. Immunohistochemical showed NRIP1 protein was higher in STAD than normal sample. Conclusions:In this study, we revealed that overexpression of NRIP1 in the STAD sample compared to normal samples, NRIP1 significantly associated with macrophage. The high expression levels of NRIP1 and more macrophage infiltration led to poor prognosis of STAD.

Knockdown of circNRIP1 sensitizes colorectal cancer to 5‑FU via sponging miR‑532‑3p

“…Emerging evidence suggested that circRNAs have a crucial role in resistance to chemotherapeutics and serve as key targets for the diagnosis and treatment of CRC (9-11). circNRIP1 was reported to be abnormally expressed in numerous tumor types and may have an important role in promoting malignant features (12)(13)(14). However, the expression and biological function of circNRIP1 in CRC has remained elusive.…”

“…Furthermore, recent studies have confirmed that circRNAs may serve as pivotal targets for the diagnosis and treatment of CRC (9)(10)(11). It has been reported that circRNA nuclear receptor-interacting protein 1 (NRIP1), a novel circRNA, is upregulated in several tumor types, including gastric cancer (12), stomach adenocarcinoma (13) and esophageal squamous cell carcinoma (14). However, studies on the expression and biological function of circNRIP1 in CRC are still lacking.…”

The Expression of NRIP1 and LCOR in Endometrioid Endometrial Cancer

“…However, low nuclear expression of LCOR and high nuclear expression of NRIP1 were also associated with higher grade cervical intraepithelial neoplasia, underlying their role in cancer development (25,26). The expression of both genes has been found to be down-regulated in colorectal cancer, while in gastric cancer, higher levels of these co-regulators were associated with poor prognosis (14,44). Triki et al observed strong correlations of NRIP1 and LCOR with E-cadherin and cyclo-oxygenase 2 expression in colorectal cancer (14).…”

Emerging Roles of Ubiquitin-Specific Protease 25 in Diseases

“…The combination of bioinformatic tools, including sequencing, and data analysis of experimental results is widely used by an increasing number of research teams to find potential clinical targets associated with tumors. Based on the analysis of available databases, the overexpression of USP25 was found to indicate a poor outcome in stomach adenocarcinoma ( Fang and Lu, 2020 ). Yamada et al analyzed the presence of putative tumor suppressor genes in the relevant genomic region due to the frequent loss of heterozygosity at 21q21 in lung cancer and the identification of homozygous deletions in this region.…”