Expression and role of epidermal growth factor receptors during differentiation of cementoblasts, osteoblasts, and periodontal ligament fibroblasts in the rat

Formation of the sphenomandibular ligament by Meckel's cartilage in the mouse: possible involvement of epidermal growth factor as revealed by studies in vivo and in vitro

“…It might result in the suppression of the activity of ALPase, which is considered to be an enzyme that is associated closely with matrix mineralization in hard tissues (Tenenbaum 1987;Kawase et al 1988;Beertsen and VandenBos 1991). Treatment with EGF has previously been reported to decrease spontaneous ALPase activity (Kumegawa et al 1983;Earp et al 1986;Kato et al 1993;Cho and Garant 1996), and the results strongly support a close relationship between ALPase activity and calcification.…”

“…EGF was initially reported as a potent mitogen of epithelial cells and mesenchymal cells and later was shown to be involved in tooth eruption, eyelid opening, and craniofacial formation (Cohen 1962;Cohen and Taylor 1974;Fisher and Lakshmanan 1990;Carpenter and Cohen 1990;Slavkin et al 1992;Wise et al 1992;Cho and Garant 1996). It can cause morphological changes in elongated cells, as reported for myoblasts and chondrocytes (Gospodarowiez and Mescher 1977;Katoh and Takayama 1984;Makower et al 1989), and it appears to be involved in the transformation of Meckel's chondrocytes.…”

“…Several investigators have demonstrated that EGF inhibits the synthesis of collagen fibrils and proteoglycans in vitro (Canalis and Raisz 1979;Prins et al 1982b;Kumegawa et al 1983;Hata et al 1984;Cho and Garant 1996). Hiramatsu et al (1982) have reported that the formation of collagen fibers in MC3T3-E1 cells is inhibited by EGF and that type I collagen with typical cross-striate fibrils is undetectedable.…”

“…Whereas EGF was shown initially to stimulate the proliferation of epithelial cells, it is currently regarded as a potent mitogen for various cell types, such as fibroblasts, osteoblasts, and myoblasts (Gospodarowicz and Mescher 1977;Carpenter and Cohen 1979;Boonstra et al 1995;Riese and Stern 1998). EGF also promotes some developmental processes, which include tooth morphogenesis and eruption, eyelid opening, palate formation, and lung maturation (Cohen 1962;Rihtniemi and Thesleff 1987;Carpenter and Cohen 1990;Fisher and Lakshmanan 1990;Wise et al 1992;Cho and Garant 1996;Shroff et al 1996;Amano et al 1999). Furthermore, there are some reports that EGF enhances DNA synthesis and mitotic activity in chondrocytes and, consequently, facilitates cell proliferation, although it can also inhibit proteoglycan synthesis and cell differentiation (Gospodarowicz and Mescher 1977;Kato et al 1983;Prins et al 1982aPrins et al , 1982bTakigawa et al 1982Takigawa et al , 1991Hiraki et al 1985Hiraki et al , 1987Mercola and Stiles 1988).…”

A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

“…1, clearly demonstrate that the established cell line PDL-L2 shares identical expression of the genes examined. Furthermore, PDL-L2 expressed the EGFR genes (data not shown), which have previously been shown to be present in the PDL (Matsuda et al, 1993;Cho and Garant, 1996). Since Runx2/Cbfa1/Osf2 is a transcription factor that is specifically expressed and functions in osteogenic cells, western blot analysis was also performed to examine whether or not this protein is produced in PDL-L2 cells.…”