Expression and regulation of M-type K+ channel in PC12 cells and rat adrenal medullary cells

Harada, Kensuke; Matsuoka, Hiroaki; Inoue, Masumi

doi:10.1007/s00441-018-2809-y

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…Similar findings have been reported in the perfused cat adrenal medulla (7). Thus, the excitable membrane properties seem to differ between AM cells cultured and in situ (47).…”

Section: Discussion K + Channels Involved In the Resting Membrane Pot...supporting

confidence: 87%

Lack of p11 expression facilitates acidity‐sensing function of TASK1 channels in mouse adrenal medullary cells

et al. 2018

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External acidity induces catecholamine secretion by inhibiting TASK1-like channels in rat adrenal medullary (AM) cells. TASK channels can function as a heteromer or homomer in the TASK subfamily. In this study, we elucidate the molecular identity of TASK1-like channels in mouse AM cells using gene knockout. Genetic deletion of TASK1, but not TASK3, abolished the depolarizing inward current and catecholamine secretion in response to acidity, whereas it did not affect the resting current level. Immunocytochemistry revealed that AM cells exhibited predominantly TASK1-like and little TASK3-like immunoreactivity. A proximity ligation assay showed that TASK1/3 heteromeric channels were not formed in AM cells or PC12 cells. However, the exogenous expression of p11 in PC12 cells resulted in the heteromeric formation of TASK isoforms, which were mainly located in the cytoplasm, and p11 was not expressed in rat adrenal medullae or PC12 cells. In AM cells, genetic deletion of TASK1 resulted in enhancement of the immunoreactivity of the TALK2 channel, but not TASK3. The results indicate that TASK1 homomeric channels function as acidity sensors in AM cells, and that function is facilitated by the lack of p11 expression.-Inoue, M., Matsuoka, H., Lesage, F., Harada, K. Lack of p11 expression facilitates acidity-sensing function of TASK1 channels in mouse adrenal medullary cells.

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“…Similar findings have been reported in the perfused cat adrenal medulla (7). Thus, the excitable membrane properties seem to differ between AM cells cultured and in situ (47).…”

Section: Discussion K + Channels Involved In the Resting Membrane Pot...supporting

confidence: 87%

Lack of p11 expression facilitates acidity‐sensing function of TASK1 channels in mouse adrenal medullary cells

et al. 2018

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“…Although adrenal GABA research started with bovine AMC cells (Bormann & Clapham, 1985;Kataoka et al, 1984), they were usually cultured in the presence of 10% or less fetal bovine serum. Thus, the expression of ion channels or receptors might have been altered under culture conditions (Benavides et al, 2004;Harada et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Although adrenal GABA research started with bovine AMC cells (Bormann & Clapham, 1985; Kataoka et al., 1984), they were usually cultured in the presence of 10% or less fetal bovine serum. Thus, the expression of ion channels or receptors might have been altered under culture conditions (Benavides et al., 2004; Harada et al., 2018). The present experiment aimed to identify allopregnanolone‐producing cells in the adrenal gland and determine the factor(s) responsible for a species difference in GABA A receptor expression in AMC cells.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms for establishment of GABA signaling in adrenal medullary chromaffin cells

Harada

Matsuoka

Toyohira

et al. 2021

Journal of Neurochemistry

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γ-Aminobutyric acid (GABA) is thought to play a paracrine role in adrenal medullary chromaffin (AMC) cells. Comparative physiological and immunocytochemical approaches were used to address the issue of how the paracrine function of GABA in AMC cells is established. GABA A receptor Clchannel activities in AMC cells of rats and mice, where corticosterone is the major glucocorticoid, were much smaller than those in AMC cells of guinea-pigs and cattle, where cortisol is the major. The extent of enhancement of GABA A receptor α3 subunit expression in rat pheochromocytoma (PC12) cells by cortisol was larger than that by corticosterone in parallel with their glucocorticoid activities. Thus, the species difference in GABA A receptor expression may be ascribed to a difference in glucocorticoid activity between corticosterone and cortisol. GABA A receptor Clchannel activity in mouse AMC cells was enhanced by allopregnanolone, as noted with that in guinea-pig AMC cells, and the enzymes involved in allopregnanolone production were immunohistochemically detected in the zona fasciculata in both mice and guinea pigs. The expression of glutamic acid decarboxylase 67 (GAD67), one of the GABA synthesizing enzymes, increased after birth, whereas GABA A receptors already developed at birth. Stimulation of pituitary adenylate cyclase-activating polypeptide (PACAP) receptors, but not nicotinic or muscarinic receptors, in PC12 cells, resulted in an increase in GAD67 expression in a protein-kinase A-dependent manner. The results indicate that glucocorticoid and PACAP are mainly responsible for the expressions of GABA A receptors and GAD67 involved in GABA signaling in AMC cells, respectively.

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Lead exerts a depression of neurotransmitter release through a blockade of voltage dependent calcium channels in chromaffin cells

Carretero,

Liccardi,

Tejedor

et al. 2024

Toxicology

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Expression and regulation of M-type K+ channel in PC12 cells and rat adrenal medullary cells

Cited by 3 publications

References 38 publications

Lack of p11 expression facilitates acidity‐sensing function of TASK1 channels in mouse adrenal medullary cells

Lack of p11 expression facilitates acidity‐sensing function of TASK1 channels in mouse adrenal medullary cells

Mechanisms for establishment of GABA signaling in adrenal medullary chromaffin cells

Lead exerts a depression of neurotransmitter release through a blockade of voltage dependent calcium channels in chromaffin cells

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