Expression and regulation of GnRHR2 gene and testosterone secretion mediated by GnRH2 and GnRHR2 within porcine testes

Ding, Haisheng; Liu, Min; Zhou, Changfan; You, Xiangbin; Suo, Zilan; Zhang, Chi; Xu, Dequan

doi:10.1016/j.jsbmb.2019.03.010

Cited by 10 publications

(9 citation statements)

References 55 publications

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“…PSMD4 effectively inhibits sperm-oocyte binding [42]. GNRHR2 is involved in regulating reproductive behaviour in pigs [43]. CES5A, GAL3ST1 and SPAG17 are essential for spermatogenesis and male fertility [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Shi

Wang

Liu

et al. 2020

J Animal Sci Biotechnol

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Background: Runs of homozygosity (ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms (SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here, we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs (F ROH), homozygosity (F HOM), genomic relationship matrix (F GRM) and pedigree (F PED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes. Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes (SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between F ROH_total and F HOM , while the lowest was − 0.083 between F GRM and F PED. The correlations between F PED and F ROH using four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes (SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4. Conclusion: F ROH can be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, F ROH can provide an alternative to inbreeding estimates. Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection.

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Section: Discussionmentioning

confidence: 99%

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Shi

Wang

Liu

et al. 2020

J Animal Sci Biotechnol

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“…Other genes were known to produce proteins having an autocrine/paracrine role in testes. GnRH2 and its receptor GnRHR2 were previously described as having an important autocrine/paracrine role in the testis biology [3,4,57,58]. In this study, only GNRHR2 was studied and its expression did not appear to be correlated with other genes previously highlighted.…”

Section: Discussionmentioning

confidence: 85%

“…Present analysis seems very relevant, even though it was performed with the human sequence data. Finally, the only result obtained after using porcine sequences, was that the GNRHR2 gene seems also regulated by NR5A1, CREB [57] and SP1 [67].…”

Section: Discussionmentioning

confidence: 97%

Correlation Networks Provide New Insights into the Architecture of Testicular Steroid Pathways in Pigs

Robic

Faraut

Fève

et al. 2021

Genes

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Steroid metabolism is a fundamental process in the porcine testis to provide testosterone but also estrogens and androstenone, which are essential for the physiology of the boar. This study concerns boars at an early stage of puberty. Using a RT-qPCR approach, we showed that the transcriptional activities of several genes providing key enzymes involved in this metabolism (such as CYP11A1) are correlated. Surprisingly, HSD17B3, a key gene for testosterone production, was absent from this group. An additional weighted gene co-expression network analysis was performed on two large sets of mRNA-seq to identify co-expression modules. Of these modules, two containing either CYP11A1 or HSD17B3 were further analyzed. This comprehensive correlation meta-analysis identified a group of 85 genes with CYP11A1 as hub gene, but did not allow the characterization of a robust correlation network around HSD17B3. As the CYP11A1-group includes most of the genes involved in steroid synthesis pathways (including LHCGR encoding for the LH receptor), it may control the synthesis of most of the testicular steroids. The independent expression of HSD17B3 probably allows part of the production of testosterone to escape this control. This CYP11A1-group contained also INSL3 and AGT genes encoding a peptide hormone and an angiotensin peptide precursor, respectively.

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“…Lung tissues were analyzed by Hematoxylin-Eosin staining (H&E). The experiments followed the procedures previously reported (32). Finally, these sections were observed under an optical microscope (Olympus, Tokyo, Japan) to detect morphological changes of lung tissues.…”

Section: Histological Assaymentioning

confidence: 99%

Mir-331-3p Inhibits PRRSV-2 Replication and Lung Injury by Targeting PRRSV-2 ORF1b and Porcine TNF-α

You

Zhang

et al. 2020

Front. Immunol.

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Porcine reproductive and respiratory syndrome (PRRS) caused by a single-stranded RNA virus (PRRSV) is a highly infectious respiratory disease and leads to huge economic losses to the swine industry worldwide. To investigate the role of miRNAs in the infection and lung injury induced by PRRSV, the differentially expressed miRNAs (DE-miRs) were isolated from PRRSV-2 infected/mock-infected PAMs of Meishan, Landrace, Pietrain, and Qingping pigs at 9, 36, and 60 hpi. Mir-331-3p was the only common DE-miR in each set of miRNA expression profile at 36 hpi. Mir-210 was one of 7 common DE-miRs between PRRSV infected and mock-infected PAMs of Meishan, Pietrain, and Qingping pigs at 60 hpi. Mir-331-3p/mir-210 could target PRRSV-2 ORF1b, bind and downregulate porcine TNF-α/STAT1 expression, and inhibit PRRSV-2 replication, respectively. Furthermore, STAT1 and TNF-α could mediate the transcriptional activation of MCP-1, VCAM-1, and ICAM-1. STAT1 could also upregulate the expression of TNF-α by binding to its promoter region. In vivo, pEGFP-N1-mir-331-3p could significantly reduce viral replication and pathological changes in PRRSV-2 infected piglets. Taken together, Mir-331-3p/mir-210 have significant roles in the infection and lung injury caused by PRRSV-2, and they may be promising therapeutic targets for PRRS and lung injury/inflammation.

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Expression and regulation of GnRHR2 gene and testosterone secretion mediated by GnRH2 and GnRHR2 within porcine testes

Cited by 10 publications

References 55 publications

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Correlation Networks Provide New Insights into the Architecture of Testicular Steroid Pathways in Pigs

Mir-331-3p Inhibits PRRSV-2 Replication and Lung Injury by Targeting PRRSV-2 ORF1b and Porcine TNF-α

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