Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

MacDonald, Jacqueline; Suzuki, Hitoshi; Master, Emma R.

doi:10.1007/s00253-012-3937-z

Cited by 48 publications

(20 citation statements)

References 100 publications

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“…Studies of the catalytic properties of various ligninolytic isoenzymes reflect distinct differences in both the culture conditions and substrate specificities associated with their transcription and kinetic constants [4], [39], [40]. For example, 7 isoforms of MnPs were isolated from C. subvermispora cultured in salt medium, whereas 4 isoenzymes were fractioned in extracts derived from wood chips.…”

Section: Discussionmentioning

confidence: 99%

Release of Pleurotus ostreatus Versatile-Peroxidase from Mn2+ Repression Enhances Anthropogenic and Natural Substrate Degradation

et al. 2012

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The versatile-peroxidase (VP) encoded by mnp4 is one of the nine members of the manganese-peroxidase (MnP) gene family that constitutes part of the ligninolytic system of the white-rot basidiomycete Pleurotus ostreatus (oyster mushroom). VP enzymes exhibit dual activity on a wide range of substrates. As Mn2+ supplement to P. ostreatus cultures results in enhanced degradation of recalcitrant compounds and lignin, we examined the effect of Mn2+ on the expression profile of the MnP gene family. In P. ostreatus (monokaryon PC9), mnp4 was found to be the predominantly expressed mnp in Mn2+-deficient media, whereas strongly repressed (to approximately 1%) in Mn2+-supplemented media. Accordingly, in-vitro Mn2+-independent activity was found to be negligible. We tested whether release of mnp4 from Mn2+ repression alters the activity of the ligninolytic system. A transformant over-expressing mnp4 (designated OEmnp4) under the control of the β-tubulin promoter was produced. Now, despite the presence of Mn2+ in the medium, OEmnp4 produced mnp4 transcript as well as VP activity as early as 4 days after inoculation. The level of expression was constant throughout 10 days of incubation (about 0.4-fold relative to β-tubulin) and the activity was comparable to the typical activity of PC9 in Mn2+-deficient media. In-vivo decolorization of the azo dyes Orange II, Reactive Black 5, and Amaranth by OEmnp4 preceded that of PC9. OEmnp4 and PC9 were grown for 2 weeks under solid-state fermentation conditions on cotton stalks as a lignocellulosic substrate. [14C]-lignin mineralization, in-vitro dry matter digestibility, and neutral detergent fiber digestibility were found to be significantly higher (about 25%) in OEmnp4-fermented substrate, relative to PC9. We conclude that releasing Mn2+ suppression of VP4 by over-expression of the mnp4 gene in P. ostreatus improved its ligninolytic functionality.

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Section: Discussionmentioning

confidence: 99%

Release of Pleurotus ostreatus Versatile-Peroxidase from Mn2+ Repression Enhances Anthropogenic and Natural Substrate Degradation

et al. 2012

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“…Genomic, transcriptomic, and proteomic data have considerably enriched our knowledge concerning the regulation and evolution of wood degradation systems (12,58,(60)(61)(62). Our transcriptomic analysis is original in that it focuses on the effect of oak-derived molecules, especially tannins, on P. chrysosporium gene expression.…”

Section: Transcriptomic Analysis (I) Overview Of Gene Expressionmentioning

confidence: 99%

Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase

Thuillier

Chibani

Bellı́

et al. 2014

Appl Environ Microbiol

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The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in the presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi, presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase (P. chrysosporium GTT2.1 [PcGTT2.1]) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been classified first as a GTT2 compared to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that the GTT2.1 isoform has evolved functionally to reduce lipid peroxidation by recognizing high-molecular-weight peroxides as substrates. Moreover, the GTT2.1 gene has been lost in some non-wood-decay fungi. This example suggests that the intracellular detoxification system evolved concomitantly with the extracellular ligninolytic machinery in relation to the capacity of fungi to degrade wood.

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“…T. versicolor is a common fungi species native to temperate climate zones that can secrete PODs, laccases, and carbohydrate-active enzymes. It has strong similarities to Phanerochaete chrysosporium and is a useful model organism for the study of white rot fungus (Barrasa et al1995;MacDonald et al2011;Macdonald et al 2012;Varma et al 2015) and for testing wood resistance to white rot decay (Plaschkies et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Profile of Lignocellulose Degradation from Trametes versicolor on Poplar Wood

Zhang

Wang

et al. 2017

BioResources

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The Trametes versicolor genome is predicted to encode many enzymes that effectively degrade lignin, making it a potentially useful tool for biopulping. However, the wood degradation mechanism of T. versicolor is not clear. To identify the enzymes that contribute to lignocellulose degradation, changes in the T. versicolor transcriptome during growth on poplar wood, relative to growth on glucose medium, were evaluated. Eight hundred and fifty-three genes were differentially expressed, with 360 genes up-regulated and 493 genes were down-regulated on poplar wood. Notably, most genes involved in lignin degradation were upregulated, including eight lignin peroxidase (LiP) genes. Genes encoding cellulose and hemicellulose degrading-enzymes were mostly downregulated, including six endo-β-1,4-glucanase genes and three cellobiohydrolase I genes. These results characterized transcriptomic changes related to lignocellulose degradation. This information could be used to develop T. versicolor as a tool to improve the efficiency of lignin degradation or to provide a theoretical foundation for a new paper pulp manufacturing process.

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Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

Cited by 48 publications

References 100 publications

Release of Pleurotus ostreatus Versatile-Peroxidase from Mn2+ Repression Enhances Anthropogenic and Natural Substrate Degradation

Release of Pleurotus ostreatus Versatile-Peroxidase from Mn2+ Repression Enhances Anthropogenic and Natural Substrate Degradation

Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase

Transcriptomic Profile of Lignocellulose Degradation from Trametes versicolor on Poplar Wood

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