Expression and protective role of two novel NACHT-containing proteins in pathogen infection

Hu, Yi; Zhang, Yu; Xue, Na; Nie, Pin; Chang, Ming Xian

doi:10.1016/j.dci.2014.05.007

Cited by 13 publications

(7 citation statements)

References 51 publications

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“…However, our previous custom PCR arrays (SABiosciences, QIAGEN company) showed that IFN2 could be detected in zebrafish embryos (Hu et al, 2014). In the present study, IFN2 expression in zebrafish embryos was examined by using the same primers from others (López-Muñoz et al, 2009;Roca et al, 2008), but different from the IFN2 primers used by Aggad et al (2009), which gave similar results to PCR arrays.…”

Section: Discussionsupporting

confidence: 66%

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MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors

Chen

Zou

et al. 2015

Developmental & Comparative Immunology

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Section: Discussionsupporting

confidence: 66%

“…ZF4 (Zebrafish embryonic fibroblast cell line) and EPC (Epithelioma papulosum cyprini) cells were maintained as described previously (Hu et al, 2014). SVCV was prepared in EPC cells at 25°C.…”

Section: Cells and Virusmentioning

confidence: 99%

MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors

Chen

Zou

et al. 2015

Developmental & Comparative Immunology

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“…Our previous work showed that overexpression of NALPL1 contributed to protection against bacterial and viral infection. Moreover, NALPL1 overexpression induced the expression of NOD1 60 . The impaired expression of NALPL1 in the NOD1-1IS −/− larvae reported here suggests that NALPL1 and NOD1 constitute a feed-forward circuit in host immune defense.…”

Section: Discussionmentioning

confidence: 96%

NOD1 deficiency impairs CD44a/Lck as well as PI3K/Akt pathway

Ren

et al. 2017

Sci Rep

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Pattern recognition receptors (PRRs) are crucial for host defense and tissue homeostasis against infecting pathogens. PRRs are highly conserved cross species, suggesting their key roles in fundamental biological processes. Though much have been learned for NOD1 receptor in the innate and adaptive immune responses, the roles of NOD1 during embryonic and larval stages remain poorly understood. Here, we report that NOD1 is necessary for the modulation of PI3K-Akt pathway and larval survival in zebrafish. Transcriptome analysis revealed that the significantly enriched pathways in NOD1 −/− zebrafish larvae were mainly involved in metabolism and immune system processes. Biochemical analysis demonstrated that NOD1 was required for the expression of CD44a that, in turn, activated the PI3K-Akt pathway during larval development. Conversely, over-expression of CD44a in NOD1-deficient zebrafish restored the modulation of the PI3K-Akt pathway and improved larval survival. Collectively, our work indicates that NOD1 plays a previously undetected protective role in larval survival through CD44a-mediated activation of the PI3K-Akt signaling.

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“…To explore the effect of NOD1 overexpression in SVCV infection in vitro, ZF4 cells were transfected with ptGFP1 or ptGFP1-NOD1 and subjected to G418 selection to enrich the GFP-positive cells. SVCV infection in the 24 multiwell plates was performed as described previously (34,35). Briefly, ZF4 cells stably transfected with ptGFP1 and ptGFP1-NOD1 were seeded overnight and then infected with SVCV with the MOI of 5, 0.5, and 0.05.…”

Section: Antiviral Assay In Vitro and In Vivomentioning

confidence: 99%

NOD1 Promotes Antiviral Signaling by Binding Viral RNA and Regulating the Interaction of MDA5 and MAVS

Zhang

et al. 2020

The Journal of Immunology

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Nucleotide oligomerization domain-like receptors (NLRs) and RIG-I-like receptors (RLRs) detect diverse pathogen-associated molecular patterns to activate the innate immune response. The role of mammalian NLR NOD1 in sensing bacteria is well established. Although several studies suggest NOD1 also plays a role in sensing viruses, the mechanisms behind this are still largely unknown. In this study, we report on the synergism and antagonism between NOD1 and MDA5 isoforms in teleost. In zebrafish, the overexpression of NOD1 enhances the antiviral response and mRNA abundances of key antiviral genes involved in RLR-mediated signaling, whereas the loss of NOD1 has the opposite effect. Notably, spring viremia of carp virus-infected NOD1 2/2 zebrafish exhibit reduced survival compared with wild-type counterparts. Mechanistically, NOD1 targets MDA5 isoforms and TRAF3 to modulate the formation of MDA5-MAVS and TRAF3-MAVS complexes. The cumulative effects of NOD1 and MDA5a (MDA5 normal form) were observed for the binding with poly(I:C) and the formation of the MDA5a-MAVS complex, which led to increased transcription of type I IFNs and ISGs. However, the antagonism between NOD1 and MDA5b (MDA5 truncated form) was clearly observed during proteasomal degradation of NOD1 by MDA5b. In humans, the interactions between NOD1-MDA5 and NOD1-TRAF3 were confirmed. Furthermore, the roles that NOD1 plays in enhancing the binding of MDA5 to MAVS and poly(I:C) are also evolutionarily conserved across species. Taken together, our findings suggest that mutual regulation between NOD1 and MDA5 isoforms may play a crucial role in the innate immune response and that NOD1 acts as a positive regulator of MDA5/MAVS normal form-mediated immune signaling in vertebrates.

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Expression and protective role of two novel NACHT-containing proteins in pathogen infection

Cited by 13 publications

References 51 publications

MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors

MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors

NOD1 deficiency impairs CD44a/Lck as well as PI3K/Akt pathway

NOD1 Promotes Antiviral Signaling by Binding Viral RNA and Regulating the Interaction of MDA5 and MAVS

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