Expression and prognostic influence of NF-κB and EGFR in esophageal cancer

Abstract

ABSTRACT. The goal of this study was to investigate the expression profiles of nuclear factor-kappa B (NF-κB) and epidermal growth factor receptor (EGFR) in esophageal cancer and to determine their association with tumor prognosis. This study included 40 esophageal cancer patients [22 men and 18 women; average age = 62.7 ± 3.9 years; tumor-node-metastasis (TNM) staging: 12 patients with stage I, 13 patients with stage II, and 15 patients with stage III disease]. Tumor tissues and tumor-adjacent tissue specimens were collected during radical resections at our hospital. Immunohistochemical staining was used to examine these tissues for NF-κB and EGFR expression. Follow-up of all patients included gathering information such as the 3-year survival rate. We found that NF-κB and EGFR expression was significantly higher in tumor tissues compared to tumor-adjacent normal tissues. Expression was not related to gender or age, but was positively associated with the degree of tumor infiltration. NF-κB and EGFR expression levels gradually increased with higher TNM stage, but this difference was not significant. Follow-up results showed that 16820 Y.Q. Zhang et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 16819-16826 (2015) patients with higher NF-κB and EGFR levels had a lower survival rate and unfavorable prognosis. In conclusion, we found that NF-κB and EGFR expression was significantly elevated during the occurrence and development of esophageal carcinoma, and expression of these factors appears to be correlated with cancer progression. Higher expression of both genes is associated with an unfavorable prognosis.

Chemoradiotherapy with extended nodal irradiation and/or erlotinib in locally advanced oesophageal squamous cell cancer: long-term update of a randomised phase 3 trial

“… 40 The expression of EGFR was reported to be significantly correlated with clinical stage, tumour invasion and poor prognosis. 27 – 29 , 41 Similarly, our study indicated that EGFR expression was associated with T stage and poor prognosis. Radiotherapy with EGFR tyrosine kinase inhibitors has been tested in many trials with encouraging results.…”

“…4a ), consistent with the findings of previous research reports. 27 – 29 When treated with erlotinib, patients with EGFR expression (+2, +3) had a significantly longer OS than patients without EGFR expression (0, +1) (median, 46.5 vs 9.5 months; P = 0.007, Fig. 4b ).…”

Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65

“…It has been reported that NF-κB is constitutively activated in high-grade squamous intraepithelial lesions and squamous cell carcinomas of human uterine cervix [ 24 ]. Numerous previous studies suggested that NF-κB activation not only contributes to the migration and invasion of cancer cells, but also affects cell survival and gene expressions related to tumor proliferation and metastasis [ 25 - 27 ]. Five subunits of NF-κB have been identified, namely, gp105/p50 (NF-B1), p100/p52 (NF-B2), p65 (RelA), RelB, and c-Rel [ 28 ].…”

Clinical efficacy and safety of apatinib as maintenance treatment in patients with advanced esophageal squamous cell carcinoma

“…VEGFR-2 and Nuclear Factor Kappa B(NF-kB)-positive substances are mainly localized in the cytoplasm and are brownyellow granular.Ten visual elds were randomly selected under high magni cation(x400),and the percentage of positive cells was calculated in each eld. The number of positive cells in the whole section was ≥5%, which was judged as positive, and <5%was negative [9]. MVD is judged rst at the low magni cation (x100), to observe the highest blood vessel density in the tumor.…”