Expression and Prognosis of Sperm-Associated Antigen 1 in Human Breast Cancer

Abstract

Background Sperm-associated antigen 1 (SPAG1) has been identified as a marker of pancreatic cancer progression and promoter of cell motility; however, its role in breast cancer is not completely understood. Methods SPAG1 expression in breast cancer tissues and normal tissues was obtained from online databases. Knockdown function assays were designed and conducted to verify the functional role of SPAG1 in breast cancer cell lines. Cell counting and MTT assays were used to assess cell proliferation. Cell flow cytometry assay was used for cell cycle phase arrest, and fluorescence microscopy was used for colony formation assessment. Results Both the mRNA and protein levels of SPAG1 were significantly higher in the breast cancer tissues than in the normal tissues. In addition, SPAG1 is significantly related to many clinicopathological features of breast cancer, such as age (>51 years), estrogen receptor (ER) (+), progesterone receptor (PR) (+), and nodal status (+), non-triple negative breast cancer (TNBC), not basal-like and not basal-like and not TNBC. Survival analysis indicates that breast cancer patients with low expression of SPAG1 had a significantly better prognosis with relapse-free survival (RFS). Functional experiment analysis revealed that knockdown of SPAG1 suppressed cell proliferation and colony-forming ability. Conclusion Our results suggested a possible role of SPAG1 in breast cancer pathogenesis.

Comprehensive analysis of SPAG1 expression as a prognostic and predictive biomarker in acute myeloid leukemia by integrative bioinformatics and clinical validation

“…Moreover, SPAG1 is an early expressed gene in pancreatic tumorigenesis and can promote the activity of cancer cells [ 17 ]. Lin et al showed that SPAG1 expression was a crucial variable related to many clinicopathological features and to RFS in breast cancer [ 38 ]. Functionally, SPAG1 acts as an inhibitor of breast cancer cell proliferation and colony formation during breast cancer pathogenesis and development [ 38 ].…”

“…Lin et al showed that SPAG1 expression was a crucial variable related to many clinicopathological features and to RFS in breast cancer [ 38 ]. Functionally, SPAG1 acts as an inhibitor of breast cancer cell proliferation and colony formation during breast cancer pathogenesis and development [ 38 ]. Since there was no deep insight into SPAG1 in AML, further mechanistic studies are essential for investigating the possible role of SPAG1 in leukemogenesis and AML development.…”