Expression and modulation of FcϵRIα and FcϵRIβ in human blood basophils

Saini, Sarbjit S.; Richardson, Jennifer J.; Wofsy, Carla; Lavens-Phillips, Sandra; Bochner, Bruce S.; MacGlashan, Donald W.

doi:10.1067/mai.2001.114653

Cited by 59 publications

(74 citation statements)

References 35 publications

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“…However, overexpression of MZF-1 alone did not decrease the β-chain gene transcription in our previous experiments [14]. We therefore examined the behaviour of FHL3 in the presence of GM-CSF, in addition to that in the presence of IL-3 (interleukin 3), which was reported to increase β-chain gene expression [23]. GM-CSF decreased the β-chain promoter activity in a transient expression assay using a reporter plasmid including nt 4180-4260, but IL-3 had no effect on it ( Figure 6A).…”

Section: Gm-csf Facilitates the Accumulation Of Fhl3 In The Nucleusmentioning

confidence: 94%

FHL3 negatively regulates human high-affinity IgE receptor β-chain gene expression by acting as a transcriptional co-repressor of MZF-1

Takahashi

Matsumoto

2005

Biochemical Journal

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The high-affinity IgE receptor FcεRI plays a key role in triggering allergic reactions. We recently reported that human FcεRI β-chain gene expression was down-regulated by a transcription factor, MZF-1, through an element in the fourth intron. In the present study, we found that this transcriptional repression by MZF-1 required FHL3 (four and a half LIM domain protein 3) as a cofactor. Yeast two-hybrid and immunoprecipitation assays demonstrated that FHL3 bound MZF-1 in vitro and in vivo. Overexpression of FHL3 in KU812 cells suppressed the β-chain promoter activity through the element in the fourth intron in an MZF-1-dependent manner. Furthermore, results from pull-down assays and gel-filtration chromatography employing nuclear extracts indicated that MZF-1 and FHL3 formed a complex of high molecular mass with some additional proteins in the nucleus. Granulocyte-macrophage colony-stimulating factor, which was reported to decrease FcεRI expression, induced the accumulation of FHL3 in the nucleus, in accordance with the repressive role of FHL3 in β-chain gene expression.

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Section: Gm-csf Facilitates the Accumulation Of Fhl3 In The Nucleusmentioning

confidence: 94%

FHL3 negatively regulates human high-affinity IgE receptor β-chain gene expression by acting as a transcriptional co-repressor of MZF-1

Takahashi

Matsumoto

2005

Biochemical Journal

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“…Since IL-3 has been demonstrated to increase synthesis and expression of the FceRIb (25,32), probably in a STAT5 dependent manner (25), it can be hypothesized that IL-3 induced STAT5 phosphorylation might affect basophil responsiveness as the bchain amplifies FceRI signaling (33). Interestingly, basophils regulate their own priming with IL-3 by releasing autocrine IL-3 after activation of the basophils with anti-IgE (34), which might affect basophil sensitivity even more.…”

Section: Discussionmentioning

confidence: 99%

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

Verweij

Sabato

Nullens

et al. 2011

Cytometry Part B Clinical

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“…The forward primer sequence 5Ј-GTGAACCTGTGTACCTGGAAGTCTT-3Ј, reverse primer sequence 5Ј-CATCCCAGTTCCTCCAACCA-3Ј, and probe sequence 5Ј-TGACTGGCTGCTCCTTCAGGCCTC-3Ј for detection of mRNA for the ␣ subunit of human Fc⑀RI have been reported elsewhere (12). Primer/ probe combinations for TLR9 have been published elsewhere (13), were checked against accession no.…”

Section: Quantitative Rt-pcr For Detection Of Fc⑀ri␣ and Tlr9 Mrnamentioning

confidence: 99%

“…The determination of Fc⑀RI␣ protein in freshly isolated or cultured pDC (5 ϫ 10 5 cells) was done as described for human basophils (12). A lysate of a single passage of rat basophilic leukemia-SX38 cells transfected with the human ␣␤␥-chains (provided by Dr. J. P. Kinet, Harvard University, Boston, MA) was also included with each Western blot experiment.…”

Section: Western Blottingmentioning

confidence: 99%

TLR9- and FcεRI-Mediated Responses Oppose One Another in Plasmacytoid Dendritic Cells by Down-Regulating Receptor Expression

Schroeder

Bieneman

Xiao

et al. 2005

The Journal of Immunology

140

160

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Plasmacytoid dendritic cells (pDC) express not only TLR9 molecules through which ligation with CpG DNA favors Th1 responses but also possess IgE receptors (FcεRI) implicated in allergen presentation and induction of Th2 responses. This dichotomy prompted an investigation to determine whether TLR9- and IgE receptor-mediated responses oppose one another in pDC by affecting receptor expression and associated functional responses. Results showed that IgE cross-linking reduced TLR9 in pDC and inhibited the capacity of these cells to secrete IFN-α when stimulated with the CpG oligodeoxynucleotide (ODN)-2216. In contrast, an ∼15-fold reduction in FcεRIα mRNA and a loss in surface protein were seen in pDC first exposed to TLR9 ligation with ODN-2216. Results indicated that type I IFNs partly mediated this effect, as rIFN-α also caused a significant ∼4-fold reduction in FcεRIα mRNA. Finally, this reduction in FcεRIα mediated by ODN-2216 correlated with a selective suppression of allergen-induced CD4+ T cell proliferation, but not of responses resulting from tetanus toxoid. Overall, these results imply mechanisms by which specific innate and IgE-dependent immune responses counterregulate one another at the dendritic cell level and may have significant impact on whether an ensuing response is either of Th1 or Th2 in nature.

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Expression and modulation of FcϵRIα and FcϵRIβ in human blood basophils

Cited by 59 publications

References 35 publications

FHL3 negatively regulates human high-affinity IgE receptor β-chain gene expression by acting as a transcriptional co-repressor of MZF-1

FHL3 negatively regulates human high-affinity IgE receptor β-chain gene expression by acting as a transcriptional co-repressor of MZF-1

STAT5 in human basophils: IL‐3 is required for its FcεRI‐mediated phosphorylation

TLR9- and FcεRI-Mediated Responses Oppose One Another in Plasmacytoid Dendritic Cells by Down-Regulating Receptor Expression

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