Expression and localization of the Na+-H+ exchanger in the guinea pig cochlea

Goto, Satoru; Oshima, Takeshi; Ikeda, Kousuke; Takasaka, Tomonori

doi:10.1016/s0378-5955(98)00191-9

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…6 E), suggesting that it is the transporter mediating Na ϩ -dependent H ϩ extrusion from the soma. These results were consistent with previous in situ hybridization (Bond et al, 1998) and immunocytochemistry (Goto et al, 1999) experiments, both of which suggested that NHE1 is the Na ϩ /H ϩ exchanger of the hair-cell soma. NHE9 was also present in hair bundles and the cytoplasm of rat vestibular hair bundles (Fig.…”

Section: Nhe6 and Nhe9 Are Expressed In Hair Bundlessupporting

confidence: 93%

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9

Hill¹,

Brett²,

Chyou³

et al. 2006

J. Neurosci.

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In hair cells of the inner ear, robust /Hϩ exchange, which we propose is performed by NHE9 in hair bundles, exploits the high-K ϩ endolymph, responds only to pH imbalance across the bundle membrane, is unaffected by the ϩ80 mV endocochlear potential, and uses mechanisms already present in the ear for K ϩ recycling. This mechanism allows the hair cell to remove H ϩ generated by Ca 2ϩ pumping without ATP hydrolysis in the cell.

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Section: Nhe6 and Nhe9 Are Expressed In Hair Bundlessupporting

confidence: 93%

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9

Hill¹,

Brett²,

Chyou³

et al. 2006

J. Neurosci.

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“…Cloned guinea pig NHE-1 to -4 partial cDNA fragments were highly homologous to the corresponding rat NHE isoforms. NHE-1 isoform mRNA was distributed in the hair cells, marginal cells, spiral ligament fibrocytes, spiral prominence cells and spiral ganglion cells (Goto et al 1999). NHE-1 localized in a variety of cochlear cells would contribute to their differential functions.…”

Section: + -H + Exchangermentioning

confidence: 96%

Gene-Based Deafness Research: Ion Transport and Hearing

Ikeda

2004

Tohoku J. Exp. Med.

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The cochlea is a sensory organ that converts physical (sound) stimulation into electrical signals. This process is fundamentally and substantially based upon the ion transport system. Here, I summarize the physiological and molecular biological aspects of transporters, channels and receptors expressed in the cochlea. With reference to these findings, recent advances in genetic research on hereditary deafness are discussed.deafness; ion transport; hereditary deafness; DNA

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“…In situ hybridization and immunohistochemical studies demonstrated the expression of α-, β-and γ-subunits of ENaC in Reissner’s membrane (Couloigner et al, 2001; Zhong and Liu, 2004), striavascularis, spiral ligament, organ of Corti and spiral limbus (Couloigner et al, 2001; Grunder et al, 2001; Zhong and Liu, 2004). NHE-3 was localized in the apical surface of strial marginal cells(Bond et al, 1998; Goto et al, 1999) . Both αand β subtypes of Na + ,K + -ATPase were found in stria vascularis, spiral limbusand Reissner’s membrane (Erichsen et al, 1996; Marcus et al, 2011; McGuirt and Schulte, 1994).…”

Section: Cochleamentioning

confidence: 99%

Regulation of sodium transport in the inner ear

Kim

Marcus

2011

Hearing Research

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Na+ concentrations in endolymph must be controlled to maintain hair cell function since the transduction channels of hair cells are cation-permeable, but not K+-selective. Flooding or fluctuations of the hair cell cytosol with Na+ would be expected to lead to cellular dysfunction, hearing loss and vertigo. This review briefly describes cellular mechanisms known to be responsible for Na+homeostasis in each compartment of the inner ear, including the cochlea, saccule, semicircular canals and endolymphatic sac. The influx of Na+into endolymph of each of the organs is likely via passive diffusion, but these pathways have not yet been identified or characterized. Na+ absorption is controlled by gate -keeper channels in the apical (endolymphatic) membrane of the transporting cells. Highly Na+-selective epithelial sodium channels (ENaC) control absorption by Reissner’s membrane, saccular extramacular epithelium, semicircular canal duct epithelium and endolymphatic sac. ENaC activity is controlled by a number of signal pathways, but most notably by genomic regulation of channel numbers in the membrane via glucocorticoid signaling. Nonselective cation channels in the apical membrane of outer sulcus epithelial cells and vestibular transitional cells mediate Na+ and parasensory K+ absorption. The K+-mediated transduction current in hair cells is also accompanied by a Na+ flux since the transduction channels are nonselective cation channels. Cation absorption by all of these cells is regulated by extracellular ATP via apical nonselective cation channels (P2X receptors). The heterogeneous population of epithelial cells in the endolymphatic sac is thought to have multiple absorptive pathways for Na+ with regulatory pathways that include glucocorticoids and purinergic agonists.

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Expression and localization of the Na+-H+ exchanger in the guinea pig cochlea

Cited by 14 publications

References 35 publications

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9

Gene-Based Deafness Research: Ion Transport and Hearing

Regulation of sodium transport in the inner ear

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Expression and localization of the Na+-H+ exchanger in the guinea pig cochlea

Cited by 14 publications

References 35 publications

Vestibular Hair Bundles Control pH with (Na+, K+)/H+Exchangers NHE6 and NHE9

Vestibular Hair Bundles Control pH with (Na+, K+)/H+Exchangers NHE6 and NHE9

Gene-Based Deafness Research: Ion Transport and Hearing

Regulation of sodium transport in the inner ear

Contact Info

Product

Resources

About

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9

Vestibular Hair Bundles Control pH with (Na⁺, K⁺)/H⁺Exchangers NHE6 and NHE9