Expression and localization of pChAT as a novel method to study cholinergic innervation of rat adrenal gland

Elnasharty, Mohamed; Sayed-Ahmed, Ahmed

doi:10.1016/j.acthis.2014.09.001

Cited by 3 publications

(2 citation statements)

References 53 publications

(72 reference statements)

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“…As a matter of fact a further ChAT variant (pChAT), lacking exons 6 to 9, has been detected in DRG neurons [ 27 , 28 ]. Our anti-ChAT antibody was not able to discriminate this specific ChAT variant, therefore the observed variations in terms of proteins could be influenced by the expression of this additional variant.…”

Section: Discussionmentioning

confidence: 99%

Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

Corsetti

Perrone-Capano

Intriago

et al. 2021

IJMS

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Dorsal root ganglia (DRG) neurons synthesize acetylcholine (ACh), in addition to their peptidergic nature. They also release ACh and are cholinoceptive, as they express cholinergic receptors. During gangliogenesis, ACh plays an important role in neuronal differentiation, modulating neuritic outgrowth and neurospecific gene expression. Starting from these data, we studied the expression of choline acetyltransferase (ChAT) and vesicular ACh transporter (VAChT) expression in rat DRG neurons. ChAT and VAChT genes are arranged in a “cholinergic locus”, and several splice variants have been described. Using selective primers, we characterized splice variants of these cholinergic markers, demonstrating that rat DRGs express R1, R2, M, and N variants for ChAT and V1, V2, R1, and R2 splice variants for VAChT. Moreover, by RT-PCR analysis, we observed a progressive decrease in ChAT and VAChT transcripts from the late embryonic developmental stage (E18) to postnatal P2 and P15 and in the adult DRG. Interestingly, Western blot analyses and activity assays demonstrated that ChAT levels significantly increased during DRG ontogenesis. The modulated expression of different ChAT and VAChT splice variants during development suggests a possible differential regulation of cholinergic marker expression in sensory neurons and confirms multiple roles for ACh in DRG neurons, both in the embryo stage and postnatally.

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Section: Discussionmentioning

confidence: 99%

Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

Corsetti

Perrone-Capano

Intriago

et al. 2021

IJMS

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“…The adrenal gland is innervated by preganglionic sympathetic neurons from the intermediolateral cell column in the thoracic spine [ 28 ]. Two immunohistochemistry studies in rats showed intense VAChT signal in medullary nerve fibers, but no signal in medullary cells or in the cortex [ 15 , 38 ]. This suggests, that the intense signal seen in the present study is generated solely by the cholinergic preganglionic neurons in the medulla.…”

Section: Discussionmentioning

confidence: 99%

In vivo vesicular acetylcholine transporter density in human peripheral organs: an [18F]FEOBV PET/CT study

et al. 2022

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Background The autonomic nervous system is frequently affected in some neurodegenerative diseases, including Parkinson’s disease and Dementia with Lewy bodies. In vivo imaging methods to visualize and quantify the peripheral cholinergic nervous system are lacking. By using [18F]FEOBV PET, we here describe the peripheral distribution of the specific cholinergic marker, vesicular acetylcholine transporters (VAChT), in human subjects. We included 15 healthy subjects aged 53–86 years for 70 min dynamic PET protocol of peripheral organs. We performed kinetic modelling of the adrenal gland, pancreas, myocardium, renal cortex, spleen, colon, and muscle using an image-derived input function from the aorta. A metabolite correction model was generated from venous blood samples. Three non-linear compartment models were tested. Additional time-activity curves from 6 to 70 min post injection were generated for prostate, thyroid, submandibular-, parotid-, and lacrimal glands. Results A one-tissue compartment model generated the most robust fits to the data. Total volume-of-distribution rank order was: adrenal gland > pancreas > myocardium > spleen > renal cortex > muscle > colon. We found significant linear correlations between total volumes-of-distribution and standard uptake values in most organs. Conclusion High [18F]FEOBV PET signal was found in structures with known cholinergic activity. We conclude that [18F]FEOBV PET is a valid tool for estimating VAChT density in human peripheral organs. Simple static images may replace kinetic modeling in some organs and significantly shorten scan duration. Clinical Trial Registration Trial registration: NCT, NCT03554551. Registered 31 May 2018. https://clinicaltrials.gov/ct2/show/NCT03554551?term=NCT03554551&draw=2&rank=1.

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3D organization of the rat adrenal medulla

Kemoklidze,

Tyumina

2024

Vitamins and Hormones

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Expression and localization of pChAT as a novel method to study cholinergic innervation of rat adrenal gland

Cited by 3 publications

References 53 publications

Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

In vivo vesicular acetylcholine transporter density in human peripheral organs: an [18F]FEOBV PET/CT study

3D organization of the rat adrenal medulla

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