Expression and localization of membrane type 1 matrix metalloproteinase in tooth tissues

Caron, C; Xue, Jing; Bartlett, John D.

doi:10.1016/s0945-053x(98)90098-1

Cited by 38 publications

(45 citation statements)

References 26 publications

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“…The detection of MT1-MMP and MMP-2 in the enamel organ and in odontoblasts is in line with a growing body of Wndings that highlight the critical role of MMPs on the formation and mineralization of dentin and enamel matrices (Caron et al 1998;Satoyoshi et al 2001;Yoshiba et al 2003;Bourd-Boittin et al 2005). MT1-MMP is a cellular receptor for and activator of pro-MMP-2, which forms a trimolecular complex on the cell surface with TIMP-2 (for review see (Seiki 1999).…”

Section: Discussionsupporting

confidence: 52%

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Schwab

Harada

Goetz

et al. 2007

Histochem Cell Biol

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In tooth development matrix metalloproteinases (MMPs) are under the control of several regulatory mechanisms including the upregulation of expression by inducers and downregulation by inhibitors. The aim of the present study was to monitor the occurrence and distribution pattern of the extracellular matrix metalloproteinase inducer (EMMPRIN), the metalloproteinases MMP-2 and MT1-MMP and caveolin-1 during the cap and bell stage of rat molar tooth germs by means of immunocytochemistry. Strong EMMPRIN immunoreactivity was detected on the cell membranes of ameloblasts and cells of the stratum intermedium in the bell stage of the enamel organ. Differentiating odontoblasts exhibited intense EMMPRIN immunoreactivity, especially at their distal ends. Caveolin-1 immunoreactivity was evident in cells of the internal enamel epithelium and in ameloblasts. Double immunofluorescence studies revealed a focal co-localization between caveolin-1 and EMMPRIN in ameloblastic cells. Finally, western blotting experiments demonstrated the expression of EMMPRIN and caveolin-1 in dental epithelial cells (HAT-7 cells). A substantial part of EMMPRIN was detected in the detergent-insoluble caveolin-1-containing low-density raft membrane fraction of HAT-7 cells suggesting a partial localization within lipid rafts. The differentiation-dependent co-expression of MMPs with EMMPRIN in the enamel organ and in odontoblasts indicates that EMMPRIN takes part in the induction of proteolytic enzymes in the rat tooth germ. The localization of EMMPRIN in membrane rafts provides a basis for further investigations on the role of caveolin-1 in EMMPRIN-mediated signal transduction cascades in ameloblasts.

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Section: Discussionsupporting

confidence: 52%

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Schwab

Harada

Goetz

et al. 2007

Histochem Cell Biol

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“…On the other hand MT1-MMP expression was demonstrated in the ameloblast and odontoblast surface membrane by Caron et al (1998) while Beertsen et al (2002Beertsen et al ( , 2003 and Bartlett et al (2003) demonstrated that in mice deficient for MT1-MMP both eruption and root elongation were severely inhibited. Beertsen et al (2002) also demonstrated in the periodontal ligament fibroblast an accumulation of phagolysosomes which represent an important step in collagen fibril degradation.…”

Section: Discussionmentioning

confidence: 99%

Increase of MT1-MMP, TIMP-2 and Ki-67 proteins in the odontogenic region of the rat incisor post-shortening procedure

et al. 2010

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MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP and TIMP-2 proteins in these processes is still to be evaluated. Male Wistar rats were divided in two groups. In the normofunctional group (NF) the lower teeth of the rats remained in a normal eruption process. In the hypofunctional group (HP) rats their lower left incisor tooth was shortened every 2 days during 12 days. The eruption rate was estimated during the shortening period and MT1-MMP, TIMP-2 and Ki-67 protein expression from the odontogenic region was measured after the treatment. In HP groups an increase in eruption rate, and in MT1-MMP/TIMP-2 and Ki-67 expression were observed. We conclude that there is a relationship between the increase in eruption rate, and in levels of MT1-MMP, TIMP-2 and Ki-67 in the HP group. This suggests that MT1-MMP and TIMP-2 may have some role in cell proliferation during the eruption of the rat incisor tooth.

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“…In primary cultures (lane 1), some of very faint bands are visible 1997; Holmbeck et al 1999), or endocytosis (Egeblads and Werb 2000;Hurley and Wendland 2002). Indeed, MMPs/MT-MMPs are localized in dental pulp (Caron et al 1998;Palosaari et al 2002), enamel organ and BM (Heikinheimo and Salo 1995;Randall and Hall 2002). For activation, MMPs are dependent on MT-MMPs or integrins in collagen gel (Ruangpanit et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture

et al. 2004

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In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odontoblast differentiation (Kikuchi et al. 2002), we examined whether dental papilla cells in rat mandibular incisors could differentiate into tubular dentine-forming cells. In our previously established organ culture system, CM-Dil-labeled cells that were microinjected into isolated dental papillae were replaced by a row of odontoblasts. In a three-dimensional cell culture system, which consists of two kinds of type I collagen in the upper layer over multi-layered cells seeded onto collagen containing Matrigel in the lower layer and which acts as a structural meshwork, dental papilla cells were incubated as multi-layered cells in an artificial extracellular matrix (ECM). The cells aggregated to form a cell mass and invaginated as a cell mass into the ECM. The cells also extended fine fibrillar processes into the ECM. With regard to invagination, the proteolytic activities of matrix metalloproteinase-2 (MMP-2)/membrane type 1-matrix metalloproteinase (MT 1-MMP) were observed on the outer multi-layers of cells within a cell mass adjacent to the ECM. The cell mass progressively shrank to about one-half to one-third of its original diameter and was organized as a tissue surrounded by a newly secreted ECM, like dental pulp-dentine. The cells adjacent to the secreted ECM were constructed as a row of polarized columnar cells. They extended slender processes into the new ECM, which is characteristic of tubular matrix. Dentine sialophosphoprotein (DSPP) and dentine matrix protein 1 (DMP 1) genes, which are specific for odontoblast differentiation, were expressed in an aggregated cell mass where tubular matrix-forming cells were induced. Furthermore, the tubular matrix became mineralized under prolonged culture. These results imply that the putative progenitor cells/stem cells residing in dental papillae can differentiate into odontoblasts under appropriate conditions in vitro.

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Expression and localization of membrane type 1 matrix metalloproteinase in tooth tissues

Cited by 38 publications

References 26 publications

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Increase of MT1-MMP, TIMP-2 and Ki-67 proteins in the odontogenic region of the rat incisor post-shortening procedure

Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture

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