Expression and localization of <i>Nlrp4g</i> in mouse preimplantation embryo

Peng, Hong-Li; Lin, Xin-Ying; Li, Wenhao; Zhang, Wenchang

doi:10.1017/s0967199414000525

Cited by 5 publications

(8 citation statements)

References 24 publications

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“…After ovulation and fertilization, the NLRP9B protein was present throughout early embryogenesis, while the protein level diminished gradually with embryo development. This expression pattern was similar to those of NLRP2, NLRP4G and NLRP5 during preimplantation embryo development [ 6 , 8 , 16 ]. Confocal microscopy revealed that the NLRP9B protein was predominantly localized in the cytoplasm of blastomeres.…”

Section: Discussionsupporting

confidence: 59%

“…Confocal microscopy revealed that the NLRP9B protein was predominantly localized in the cytoplasm of blastomeres. This distribution of NLRP9B protein is similar to those of NLRP2 and NLRP4G [ 6 , 16 ] and differs from that of NLRP5, which is mainly located in the subcortical regions of oocytes and preimplantation embryos [ 3 , 4 ].…”

Section: Discussionmentioning

confidence: 98%

“…Knockdown of Nlrp2 or Nlrp14 expressions in the zygote led to most zygotes being arrested at the 1-cell to 8-cell stage [ 6 , 9 ]. Besides Nlrp2 , Nlrp5 and Nlrp14 , Nlrp4a–Nlrp4g also show specificity or are mainly expressed in the ovary [ 14 , 16 ]. The latest research has indicated that the Nlrp9a–Nlrp9c transcripts exist predominantly in oocytes and early embryos [ 15 ].…”

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confidence: 99%

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NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse

Peng

Lin

Liu

et al. 2015

Journal of Reproduction and Development

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Nlrp9a, Nlrp9b and Nlrp9c are preferentially expressed in oocytes and early embryos in the mouse. Simultaneous genetic ablation of Nlrp9a and Nlrp9c does not affect early embryonic development, but the function of Nlrp9b in the process of oocyte maturation and embryonic development has not been elucidated. Here we show that both Nlrp9b mRNA and its protein are expressed in ovaries and the small intestine. Moreover, the NLRP9B protein was restricted to oocytes in the ovary and declined with oocyte aging. After ovulation and fertilization, NLRP9B protein was found in preimplantation embryos. Confocal microscopy demonstrated that it was mainly localized in the cytoplasm in the oocytes and blastomeres. Thus, this protein might play a role in oocyte maturation and early embryonic development. However, knockdown of Nlrp9b expression in GV-stage oocytes using RNA interference did not affect oocyte maturation or subsequent parthenogenetic development after Nlrp9b-deficient oocytes were activated. Furthermore, Nlrp9b knockdown zygotes could reach the blastocyst stage after being cultured for 3.5 days in vitro. These results provide the first evidence that the NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 98%

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confidence: 99%

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NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse

Peng

Lin

Liu

et al. 2015

Journal of Reproduction and Development

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“…Mice and humans have 14 and 20 NLRP-encoding genes [5][6][7], respectively, with a subset being expressed in oocytes and early preimplantation embryos. Of the 14 human NLRP genes, ten NLRPs (2,4,5,7,8,9,11,12,13,14) were detected in oocytes and/or early embryos [7]. Mutations in NLRP genes are associated with different disorders, including developmental diseases [3].…”

Section: Introductionmentioning

confidence: 99%

Maternally contributed Nlrp9b expressed in human and mouse ovarian follicles contributes to early murine preimplantation development

Amoushahi

Steffensen

Galieva

et al. 2020

J Assist Reprod Genet

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Purpose The aim of the study is to investigate presence and role of the gene encoding the maternally contributed nucleotidebinding oligomerization domain (NOD)-like receptors with a pyrin domain (PYD)-containing protein 9 (NLRP9) in human and mouse ovaries, respectively, and in preimplantation mouse embryo development by knocking down Nlrp9b. Methods Expression levels of NLRP9 mRNA in human follicles were extracted from RNA sequencing data from previous studies. In this study, we performed a qPCR analysis of Nlpr9b mRNA in mouse oocytes and found it present. Intracellular ovarian distribution of NLRP9B protein was accomplished using immunohistochemistry. The distribution of NLRP9B was explored using a reporter gene approach, fusing NLRP9B to green fluorescent protein and microinjection of in vitrogenerated mRNA. Nlrp9b mRNA function was knocked down by microinjection of short interference (si) RNA targeting Nlrp9b, into mouse pronuclear zygotes. Knockdown of the Nlrp9b mRNA transcript was confirmed by qPCR. Result We found that the human NLRP9 gene and its corresponding protein are highly expressed in human primordial and primary follicles. The NLRP9B protein is localized to the cytoplasm in the blastomeres of a 2-cell embryo in mice. SiRNAmediated knockdown of Nlrp9b caused rapid elimination of endogenous Nlrp9b mRNA and premature embryo arrest at the 2-to 4-cell stages compared with that of the siRNA-scrambled control group. Conclusions These results suggest that mouse Nlrp9b, as a maternal effect gene, could contribute to mouse preimplantation embryo development. It remains to investigate whether NLRP9 have a crucial role in human preimplantation embryo and infertility.

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“…Once activated, NLRP1 specifically recruits and activates caspase‐1 downstream, which subsequently cleaves pro‐IL‐1β and pro‐IL‐18 and promotes the maturation and release of IL‐1β and IL‐18 (Martinon et al, ). Several lines of evidence show that NLRP2, NLRP4, NLRP5, and NLRP7 play important roles in embryonic development (Bauernfeind et al, ; Murdoch et al, ; Peng et al, ; Zhang et al, ). NLRP3 is the most‐studied member of the NLR family of proteins.…”

Section: Introductionmentioning

confidence: 99%

The function of NOD‐like receptors in central nervous system diseases

Kong

Yuan

Cheng

2016

J of Neuroscience Research

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NOD-like receptors (NLRs) are critical cytoplasmic pattern-recognition receptors (PRRs) that play an important role in the host innate immune response and immunity homeostasis. There is a growing body of evidence that NLRs are involved in a wide range of inflammatory diseases, including cancer, metabolic diseases, and autoimmune disorders. Recent studies have indicated that the proteins of the NLR family are linked with the pathophysiology of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and multiple sclerosis (MS), and psychological diseases. In this review, we mainly focus on the role of NLRs and the underlying signaling pathways in central nervous system (CNS) diseases. © 2016 Wiley Periodicals, Inc.

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Expression and localization of Nlrp4g in mouse preimplantation embryo

Cited by 5 publications

References 24 publications

NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse

NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse

Maternally contributed Nlrp9b expressed in human and mouse ovarian follicles contributes to early murine preimplantation development

The function of NOD‐like receptors in central nervous system diseases

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