Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles

Berisha, Bajram; Sinowatz, Fred; Schams, D.

doi:10.1002/mrd.10386

Cited by 111 publications

(99 citation statements)

References 40 publications

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“…FGF-1, -2, -4, -8, -9 and -13 efficiently activate FGFR3c and -4 (Ornitz et al 1996, Greene et al 1998. In cattle, Fgf1 and Fgf2 expression was predominantly localized to theca cells (Berisha et al 2004), and Fgf2 expression increased with estradiol content (Berisha et al 2004) as did the expression of Fgfr3c in granulosa cells in the present study. FGF2 binding is not very specific, in the sense that it activates receptors 1, 2c, 3c and 4 (Ornitz et al 1996).…”

Section: Fgfr3csupporting

confidence: 62%

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Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles

Buratini¹,

Teixeira²,

Costa³

et al. 2005

Reproduction

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Paracrine cell signaling is believed to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family has been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR3c and FGFR4) are expressed in bovine antral follicles. RT-PCR was used to analyze bovine Fgf8, Fgfr3c and Fgfr4 mRNA levels in oocytes, and granulosa and theca cells. Fgf8 expression was detected in oocytes and in granulosa and theca cells; this expression pattern differs from that reported in rodents. Granulosa and theca cells, but not oocytes, expressed Fgfr3c, and expression in granulosa cells increased significantly with follicle estradiol content, a major indicator of follicle health. Fgfr4 expression was restricted to theca cells in the follicle, and decreased significantly with increasing follicle size. To investigate the potential regulation of Fgfr3c expression in the bovine granulosa, cells were cultured in serum-free medium with FSH or IGF-I; gene expression was upregulated by FSH but not by IGF-I. The FSH-responsive and developmentally regulated patterns of Fgfr3c mRNA expression suggest that this receptor is a potential mediator of paracrine signaling to granulosa cells during antral follicle growth in cattle. IntroductionAntral ovarian follicle growth in monovular species is regulated by a number of factors, the most well known of which are the gonadotropins. Follicles are considered to be follicle-stimulating hormone (FSH)-dependent until dominance occurs, after which they become luteinizing hormone-dependent (reviewed by Fortune et al. 2001, Ginther et al. 2001. It has also become clear that growth factors are key stimulatory/regulatory molecules. Several lines of evidence point to a critical role for members of the transforming growth factor-b (TGF-b) superfamily, especially growth/differentiation factor 9 and bone morphogenetic protein 15 (reviewed by Gilchrist et al. 2004, Juengel et al. 2004, Shimasaki et al. 2004.The fibroblast growth factor (FGF) family is emerging as a group of factors that are potentially important for follicle growth. For example, FGF-7 is expressed in theca cells, its receptor is expressed in granulosa cells (Parrott & Skinner 1998, Berisha et al. 2004, and FGF-7 stimulated bovine granulosa cell proliferation and inhibited steroidogenesis (Parrott & Skinner 1998). Another potentially interesting member of this family is FGF-8. Widely expressed in fetal tissues, this factor is predominantly expressed in the gonads of adult rodents and ruminants (MacArthur et al. 1995a, Buratini et al. 2005. Within the ovary, Fgf8 gene expression occurs only in the oocyte in adult mice (Valve et al. 1997), which suggests a potential role in signaling of follicular cells by the oocyte.There are five known FGF receptor (FGFR) genes (Kim et al. 2001, Sleeman et al. 2001, of which FGF-8 preferentially activates FGFR4 and the 'c' splice form of FGFR3 (Ornitz et al. 1996). mRNAs encoding Fgfr4 or Fgfr3c were not consiste...

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Section: Fgfr3csupporting

confidence: 62%

“…For example, FGF-7 is expressed in theca cells, its receptor is expressed in granulosa cells (Parrott & Skinner 1998, Berisha et al 2004, and FGF-7 stimulated bovine granulosa cell proliferation and inhibited steroidogenesis (Parrott & Skinner 1998). Another potentially interesting member of this family is FGF-8.…”

Section: Introductionmentioning

confidence: 99%

Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles

Buratini¹,

Teixeira²,

Costa³

et al. 2005

Reproduction

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“…For the immunohistochemical demonstration of eNOS and iNOS, tissue samples were fixed in Bouin's solution for 12 h, as described previously (Berisha et al 2004). The specimens were dehydrated and embedded in paraffin wax.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Ulbrich¹,

Rehfeld²,

Bauersachs³

et al. 2006

Journal of Endocrinology

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Nitric oxide synthases (NOS) account for the endogenous production of nitric oxide (NO), a small and permeable bioreactive molecule. NO is known to act as a paracrine mediator during various processes associated with female reproduction. In the present study, the mRNA expression of the endothelial (eNOS) and inducible (iNOS) NO synthases were examined in bovine oviduct epithelial cells (BOEC) during the oestrous cycle. In addition, eNOS and iNOS mRNA and protein were localised by in situ hybridisation and immunocytochemistry respectively. Furthermore, the effects of exogenously applied oestradiol-17 and progesterone on NOS mRNA regulation were studied in a suspension culture of BOEC. The eNOS mRNA abundance was low around ovulation (day 0) and increased significantly until pro-oestrus (day 18) in the ampulla. Immunoreactive protein of eNOS was detected predominantly in endothelial cells as well as in secretory oviduct epithelial cells at pro-oestrus. The iNOS mRNA concentration was significantly reduced in the isthmus at pro-oestrus (day 18) and oestrus (day 0) compared with persistently high levels in the ampulla. By in situ hybridisation, specific iNOS transcripts were additionally demonstrated in the oviduct epithelium. Immunoreactive iNOS protein was localised in secretory epithelial cells as well as in the lamina muscularis. The in vitro stimulation showed that both NOS were stimulated by progesterone, but not by oestradiol-17 . The region-specific modulated expression of eNOS and iNOS provides evidence for an involvement of endogenously produced NO in the regulation of oviductal functions.

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“…The intrafollicular factors that are candidates for activation of deviation include those related to the insulin-like growth factor (IGF) system, steroids, inhibin-A/activin-A peptides, gonadotrophin receptors, angiogenic factors and several other intrafollicular factors (Armstrong & Webb 1997, Berisha et al 2003, Ireland et al 2004. However, the only factors, which have been temporally or functionally implicated in deviation are IGF-I and its associated system, oestradiol and LH receptors.…”

Section: Introductionmentioning

confidence: 99%

Follicle selection in cattle and horses: role of intrafollicular factors

Beg¹,

Ginther²

2006

Reproduction

143

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The eminent event in follicle selection during a follicular wave in monovular species is diameter deviation, wherein one follicle continues to grow (developing dominant) and other follicles (subordinates) begin to regress. In cattle, the IGF system, oestradiol and LH receptors are involved in the intrafollicular events initiating deviation as indicated by the following: (1) concentrations of free IGF-I and oestradiol in the follicular fluid and number of LH receptors in the follicular wall increase more dramatically in the future dominant follicle than in the future subordinate follicles before the beginning of deviation and (2) ablation of the largest follicle (LF) or injection of recombinant human IGF (rhIGF)-I into the second LF at the expected beginning of deviation increases the concentrations of oestradiol in second LF before the expected beginning of deviation between second LF and third LF. In horses, an increase in free IGF-I, oestradiol, inhibin-A and activin-A is greater in the future dominant follicle than in other follicles before the beginning of deviation. However, free IGF-I is the only one of these four factors needed for the initiation of deviation in horses as indicated by the following: (1) ablation of LF at the expected beginning of deviation increases the concentrations of free IGF-I in second LF before the beginning of deviation between second LF and third LF but does not increase the other factors; (2) injection of rhIGF-I into second LF at the expected beginning of deviation causes second LF to continue to grow and become a codominant follicle and (3) injection of IGF-binding protein-3 into LF at the expected beginning of deviation causes LF to regress and second LF to become dominant. Thus, the dramatic changes in the IGF system in LF compared to other follicles before the beginning of deviation play a crucial role in the events that lead to the beginning of diameter deviation in both cattle and horses. Oestradiol and LH receptors also play a role in cattle. These intrafollicular events prepare the selected follicle for the decreasing availability of FSH and increasing availability of LH. The other follicles of the wave have the same future capability but do not have adequate time to attain a similar preparatory stage.

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Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles

Cited by 111 publications

References 40 publications

Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles

Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Follicle selection in cattle and horses: role of intrafollicular factors

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