Expression and localization of collagen type IV α1 chain in medaka ovary

Kato, Yumiko; Ogiwara, Katsueki; Fujimori, Chika; Kimura, Atsushi; Takahashi, Takayuki

doi:10.1007/s00441-010-0969-5

Cited by 16 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we observed that the collagen IVα5 gene is also expressed at the periphery of the ovarian lamellae during early ovarian differentiation (unpubl. data), and collagen IV is known to be expressed by theca cells in the medaka ovary [Kato et al, 2010]. These similarities between rainbow trout and medaka suggest that the cells expressing both cyp19a1a and fst during rainbow trout early ovarian development are also precursors of theca cells.…”

Section: Discussionmentioning

confidence: 69%

Follistatin Is an Early Player in Rainbow Trout Ovarian Differentiation and Is Both Colocalized with Aromatase and Regulated by the Wnt Pathway

Nicol

Yano

Jouanno

et al. 2013

Sex Dev

View full text Add to dashboard Cite

In mammals, follistatin (FST) plays an important role in early ovarian differentiation, acting downstream of the Wnt pathway. In teleost fish, fst is implicated in folliculogenesis and oocyte maturation, and an early and specific expression during ovarian differentiation has been described in rainbow trout, Oncorhynchus mykiss. By in situ hybridization, we demonstrated that during rainbow trout gonadal differentiation, fst shares a similar expression pattern with cyp19a1a, the gene encoding ovarian aromatase, a key steroidogenic enzyme needed for ovarian differentiation in fish. Expression of fst and cyp19a1a was first detected in a few scattered cells in the embryonic ovary several days before hatching. Then, after histological differentiation, fst and cyp19a1a expression was localized in clusters of cells lining the future ovarian lamellae. As FST expression is known to be induced by the Wnt/β-catenin pathway in mammals, the Wnt pathway was inhibited in vivo with the IWR-1 molecule, and we analyzed by qPCR the effects of this treatment on fst expression. We found that IWR-1 decreased fst expression in female gonads, consistent with a regulation of fst expression by the Wnt pathway in rainbow trout. Furthermore, expression of cyp19a1a was also downregulated, suggesting an implication of the Wnt pathway in ovarian differentiation.

show abstract

Section: Discussionmentioning

confidence: 69%

Follistatin Is an Early Player in Rainbow Trout Ovarian Differentiation and Is Both Colocalized with Aromatase and Regulated by the Wnt Pathway

Nicol

Yano

Jouanno

et al. 2013

Sex Dev

View full text Add to dashboard Cite

show abstract

“…2E). We also analyzed the transcripts of gelatinase B ( mmp9 ), a marker for granulosa cells of the large follicles [25], and collagen type I α1-chain and collagen type IV α1-chain, markers for theca cells [26], [27]. A fragment of mmp9 , but not of collagen type I α1-chain or collagen type IV α1-chain, was amplified, confirming that lhcgrbb mRNA, but not fshra mRNA, is expressed in the granulosa cells of the large follicles.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

et al. 2013

Self Cite

View full text Add to dashboard Cite

The molecular properties and roles of luteinizing hormone (Lh) and its receptor (Lhcgrbb) have not been studied for the medaka (Oryzias latipes), which is an excellent animal model for ovulation studies. Here, we characterized the medaka Lh/Lhcgrbb system, with attention to its involvement in the ovulatory process of this teleost fish. In the medaka ovary, follicle-stimulating hormone receptor mRNA was expressed in small and medium-sized follicles, while lhcgrbb mRNA was expressed in the follicle layers of all growing follicles. Experiments using HEK 293T cells expressing medaka Lhcgrbb in vitro revealed that gonadotropin from pregnant mare’s serum and medaka recombinant Lh (rLh) bound to the fish Lhcgrbb. The fish gonadotropin subunits Gtha, Fshb, and Lhb were essentially expressed at fairly constant levels in the pituitary of the fish during a 24-h spawning cycle. Using medaka rLh, we developed a follicle culture system that allowed us to follow the whole process of oocyte maturation and ovulation in vitro. This follicle culture method enabled us to determine that the Lh surge for the preovulatory follicle occurred in vivo between 19 and 15 h before ovulation. The present study also showed that oocyte maturation and ovulation were delayed several hours in vitro compared with in vivo. Treatment of large follicles with medaka rLh in vitro significantly increased the expression of Mmp15, which was previously demonstrated to be crucial for ovulation in the fish. These findings demonstrate that Lh/Lhcgrbb is critically involved in the induction of oocyte maturation and ovulation.

show abstract

“…It is generally accepted that ECM proteins present in the follicle layers of ovulating follicles are collagen type I, which is associated with the theca cell layer, whereas collagen type IV and laminin are two major components of the follicle basement membrane. Our recent studies have indicated that collagen type I [32] and collagen type IV [33] are localized in the follicle layer of the follicle in the medaka. In addition, gelatinase A was activated by Mmp14 at the plasma membrane of the oocyte to hydrolyze collagen type IV, whereas Mmp15 degraded collagen type I [17].…”

Section: Discussionmentioning

confidence: 99%

Apparent Involvement of Plasmin in Early-Stage Follicle Rupture During Ovulation in Medaka1

Ogiwara¹,

Minagawa²,

Takano³

et al. 2012

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

Until recently, the role of the proteolytic system involving serine proteases in follicle rupture during ovulation in mammalian species has been a subject of controversy. We undertook the present study to examine whether proteases play a role in follicle rupture using the teleost medaka (Oryzias latipes) model. Various serine protease inhibitors, including a specific plasmin inhibitor, drastically reduced the rate of ovulation, as assessed by an in vitro ovulation assay, which was established for the fish. Biochemical, molecular biological, and immunological analyses demonstrated that plasminogen/plasmin was present in large follicles destined to ovulate. The active protease, plasmin, was detected in follicles approximately 3-7 h before the expected time of ovulation. Specific antibodies against the medaka plasmin light chain suppressed the ovulation rate of the follicles when antibodies were added to the medium during the period in which active plasmin was generated. This finding was an indication that a plasmin-like protease similar if not identical to plasmin plays a role in follicle rupture during ovulation in the medaka. Our data also indicate that this serine protease participates in the rupture for only a few hours prior to the activation of matrix metalloproteinase (Mmp)-mediated hydrolysis at ovulation. Based on our previous and current data, we propose a follicle rupture model involving two different proteolytic enzyme systems, serine protease and Mmp, in medaka ovulation. The current study is the first to provide evidence of the indispensable role of plasmin or a plasmin-like protease in the ovulation of a nonmammalian vertebrate species.

show abstract

Expression and localization of collagen type IV α1 chain in medaka ovary

Cited by 16 publications

References 39 publications

<b><i>Follistatin</i></b> Is an Early Player in Rainbow Trout Ovarian Differentiation and Is Both Colocalized with <b><i>Aromatase</i></b> and Regulated by the Wnt Pathway

<b><i>Follistatin</i></b> Is an Early Player in Rainbow Trout Ovarian Differentiation and Is Both Colocalized with <b><i>Aromatase</i></b> and Regulated by the Wnt Pathway

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

Apparent Involvement of Plasmin in Early-Stage Follicle Rupture During Ovulation in Medaka1

Contact Info

Product

Resources

About