Expression and Localization of Aquaporin 1a in the Sea-Bass (Dicentrarchus labrax) during Ontogeny

Giffard‐Mena, Ivone; Boulo, Viviane; Abed, Charline; Cramb, Gordon; Charmantier, Guy

doi:10.3389/fphys.2011.00034

Cited by 17 publications

(6 citation statements)

References 71 publications

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“…; Giffard‐Mena et al. ). The experimental fish were within this size range, and therefore, homogeneous in terms of ontogenetic stage and any age differences could be assumed to be very small.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Temperature on the Antipredator Behaviour and on the Cholinergic Expression in the European Sea Bass (Dicentrarchus labrax L.) Juveniles

et al. 2013

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In this paper, an experimental approach was used to test for the parallel effects of temperature (T) increase on the antipredator behaviour and the cholinergic expression in the juvenile European sea bass (Dicentrarchus labrax L.). The effects of three T treatments (18, 22 and 26°C) were tested on the main behavioural components of the antipredator response towards live aquatic predators and aerial simulated attacks, whereas brain cholinergic expression was evaluated by choline acetyltransferase (ChAT) immunoblotting (Western blot) at the extreme values of the thermal range (18 and 26°C). Antipredator responses towards a live fish were analysed over pre‐exposure and exposure phases within a short temporal scale (20 s before and after the stimulus). The results suggest that T modulates several quantitative components of the antipredator behaviour. The mean shoaling index (shoal cohesiveness) was higher at 22°C than at 18 and 26°C during both the pre‐stimulus and the exposure phase. Conversely, the mean distances from the predator and the tank bottom were, respectively, lower and higher at 26°C than in the other two treatments. In regard to the antipredator response on the aerial stimulus, comparisons across treatments revealed statistically significant differences between fish performing freezing or latency to recovery, suggesting that the fright reaction has a higher persistence at the coldest T (18°C) than at 22 and 26°C. Western blot analysis revealed a reduction in brain ChAT expression in fish acclimated to 26°C compared to those at 18°C. Results were discussed in the light of the relationships between behavioural traits, metabolism and their consequences on the population level, as a response to climate change in coastal habitats.

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Section: Discussionmentioning

confidence: 99%

“…This phase corresponds to the so called ‘post‐larval stage’, which is the metamorphic transition to the juvenile stage that occurs at a body size ranging from 38 to 48 mm, according to Giffard‐Mena et al. () and above 26 mm according to Varsamos et al. ().…”

Section: Methodsmentioning

confidence: 99%

Effects of Temperature on the Antipredator Behaviour and on the Cholinergic Expression in the European Sea Bass (Dicentrarchus labrax L.) Juveniles

et al. 2013

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“…To measure the expression level of the branchial Na + /K + ‐ATPase (NKA), the gills of 10 organisms were sacrificed from each salinity treatment following the procedures described by Giffard‐Mena, Boulo, Abed, Cramb, and Charmantier (), Ostrowski et al () and Pérez‐Robles et al (). For this purpose, 100 mg of all the gill tissue (filaments and lamellae) were weighed, and total RNA extractions were performed using the TRIzol ® method (Invitrogen TM , CA, USA) following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Fluctuating salinity effect onSphoeroides annulatus(Jenyns 1842) physiological responses

Pérez‐Robles¹,

Re‐Araujo²,

Cortez‐García³

et al. 2018

Aquac Res

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Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudden salinity changes in their natural environment, which is more common than the exception, so they must adapt to survive and cope with extreme salt conditions. Therefore, Bullseye puffer juveniles were exposed to short-term stress (39 hr) by fluctuating salinity conditions (41, 35, 29, 23, 17, 11, 5, 11, 17, 23, 29, 35, 41 psu) with a 3-hr interval between each point at 26 ± 1°C in a respirometer chamber and acclimation reservoirs. Responses to oxygen consumption rate (OCR:oxygen-nitrogen atomic ratio (O:N 17-30), osmoregulatory pattern (blood osmotic pressure from 342.4 to 332.8 mmol/kg) and changes in expression levels of Na + /K + -ATPase in the gills (higher values at higher salinities) were measured. Although some signs of stress were detected below the iso-osmotic point (11.4 psu), the puffer fish is a strong euryhaline fish that survives under these conditions. Nonetheless, it could recover when salinity returned to the initial acclimation point because Sphoeroides annulatus is able to live in a wide range of environments with wide natural salinity fluctuations; thus, a common practice in aquaculture has been to expose fish to low salinity for several reasons discussed in this study. This capacity reveals its high plasticity to saline adaptation from 41 to 5 psu an up from 5 to 41 psu, all in less than 2 days. K E Y W O R D S

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“…According to Giffard‐Mena et al. (), this size corresponds to fish that are approximately 2 yr old. Upon arrival at the laboratory, the fish were randomly allocated to 180 L (100 × 40 × 50 cm) indoor well‐aerated glass aquaria (12 fish per tank, designated ‘home tank’) supplied with recirculated and filtered seawater (300 l/hr), with salinity averaging 15 ± 1 (SE) ‰ and a temperature of 19 ± 1 (SE) °C, replicating the housing conditions of the hatchery that provided the fish.…”

Section: Methodsmentioning

confidence: 99%

The Acclimation of European Sea Bass (Dicentrarchus labrax) to Temperature: Behavioural and Neurochemical Responses

et al. 2014

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Studies on fish behavioural and neurophysiological responses to water temperature change may contribute to an improved understanding of the ecological consequences of global warming. We investigated behavioural and neurochemical responses to water temperature in European sea bass (Dicentrarchus labrax) acclimated to three temperatures (18, 22 and 28°C). After 21 d of acclimation, three groups of 25 fish each were exposed to four behavioural challenges (foraging, olfactory, aversive and mirror tests). The expression of choline acetyltransferase (ChAT) was then analysed by Western blotting in CNS homogenates (from a subset of the same fish) as a marker for cholinergic system activity. In both foraging and olfactory tests, fish acclimated to 28°C exhibited significantly higher arousal responses than fish acclimated to lower temperatures. All specimens showed fright behaviour in the aversive test, but the latency of the escape response was significantly less in the fish at 28°C. Finally, the highest mirror responsiveness was exhibited by the fish acclimated to 22°C. As in the case of cholinergic neurotransmission, significantly higher ChAT levels were detected in the telencephalon, diencephalon, cerebellum and spinal cord of fish acclimated to 22 or 28°C in comparison with those maintained at 18°C. Lower ChAT levels were detected in the mesencephalon (optic tectum) at 22 and 28°C than at 18°C. These data indicate that neuronal functions are affected by water temperature. Increases or decreases in ChAT expression can be related to the functional modulation of brain and spinal cord centres involved in behavioural responses to temperature change. Overall, the results of this study suggest that the environmental temperature level influences behaviour and CNS neurochemistry in the European sea bass.

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Expression and Localization of Aquaporin 1a in the Sea-Bass (Dicentrarchus labrax) during Ontogeny

Cited by 17 publications

References 71 publications

Effects of Temperature on the Antipredator Behaviour and on the Cholinergic Expression in the European Sea Bass (Dicentrarchus labrax L.) Juveniles

Effects of Temperature on the Antipredator Behaviour and on the Cholinergic Expression in the European Sea Bass (Dicentrarchus labrax L.) Juveniles

Fluctuating salinity effect onSphoeroides annulatus(Jenyns 1842) physiological responses

The Acclimation of European Sea Bass (Dicentrarchus labrax) to Temperature: Behavioural and Neurochemical Responses

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