Expression and Localization of a <i>Rhizobium</i>-Derived Cambialistic Superoxide Dismutase in Pea (<i>Pisum sativum</i>) Nodules Subjected to Oxidative Stress

Asensio, Aaron C.; James, Euan K.; Ariz, Idoia; Arrese‐Igor, Cesar; Tejo, Pedro María Aparicio; Arredondo‐Peter, Raúl; Morán, José F.

doi:10.1094/mpmi-10-10-0253

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…Superoxide dismutase activity (SOD) activity of roots and shoots of soybean was measured in gel as described by Beauchamp and Fridovich () and Asensio et al (, ). Mitochondrial antioxidant manganese SOD (MnSOD) isoform identification was achieved according to known mobility of SODs on native gels and based on the differential inhibition of SOD activity on gels pre‐incubated with either 3‐mM KCN, which inhibited the CuZnSODs, or 5‐mM H 2 O 2 for 1 h, which inhibited FeSOD (Asensio et al , Asensio et al ). The in‐gel SOD activity assays were performed at least three times to ensure the consistency of the results.…”

Section: Methodsmentioning

confidence: 99%

Drought tolerance response of high‐yielding soybean varieties to mild drought: physiological and photochemical adjustments

Buezo

Sanz‐Sáez

Morán

et al. 2018

Physiologia Plantarum

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Soybean is a crop of agronomic importance that requires adequate watering during its growth to achieve high production. In this study, we determined physiological, photochemical and metabolic differences in five soybean varieties selected from the parental lines of a nested association mapping population during mild drought. These varieties have been described as high yielding (NE3001, HY1; LD01‐5907, HY2) or drought tolerant (PI518751; HYD1; PI398881, HYD2). Nevertheless, there has been little research on the physiological traits that sustain their high productivity under water‐limited conditions. The results indicate that high‐yielding varieties under drought cope with the shortage of water by enhancing their photoprotective defences and invest in growth and productivity, linked to a higher intrinsic water use efficiency. This is the case of the variety N‐3001 (HY1), with a tolerance strategy involving a faster transition into the reproductive stage to avoid the drought period. The present study highlights the role of the physiological and biochemical adjustments of various soybean varieties to cope with water‐limited conditions. Moreover, the obtained results underscore the fact that the high phenotypic plasticity among soybean phenotypes should be exploited to compensate for the low genetic variability of this species when selecting plant productivity in constrained environments.

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Section: Methodsmentioning

confidence: 99%

Drought tolerance response of high‐yielding soybean varieties to mild drought: physiological and photochemical adjustments

Buezo

Sanz‐Sáez

Morán

et al. 2018

Physiologia Plantarum

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“…In gel SOD activity of roots and shoots of M. truncatula was measured as described by Beauchamp and Fridovich ( 1971 ), and Asensio et al ( 2011 , 2012 ). SOD isoforms identification were made according to known mobility of SOD on native gels and based on the differential inhibition of SOD activity on gels pre-incubated with either 3 mM KCN, which inhibited the CuZnSODs, or 5 mM H 2 O 2 for 1 h, which inhibited FeSOD (Asensio et al, 2011 , 2012 ). The in-gel SOD activity assays were performed at least three times in order to ensure the consistency of the results.…”

Section: Methodsmentioning

confidence: 99%

Both Free Indole-3-Acetic Acid and Photosynthetic Performance are Important Players in the Response of Medicago truncatula to Urea and Ammonium Nutrition Under Axenic Conditions

et al. 2016

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We aimed to identify the early stress response and plant performance of Medicago truncatula growing in axenic medium with ammonium or urea as the sole source of nitrogen, with respect to nitrate-based nutrition. Biomass measurements, auxin content analyses, root system architecture (RSA) response analyses, and physiological parameters were determined. Both ammonium and ureic nutrition severely affected the RSA, resulting in changes in the main elongation rate, lateral root development, and insert position from the root base. The auxin content decreased in both urea- and ammonium-treated roots; however, only the ammonium-treated plants were affected at the shoot level. The analysis of chlorophyll a fluorescence transients showed that ammonium affected photosystem II, but urea did not impair photosynthetic activity. Superoxide dismutase isoenzymes in the plastids were moderately affected by urea and ammonium in the roots. Overall, our results showed that low N doses from different sources had no remarkable effects on M. truncatula, with the exception of the differential phenotypic root response. High doses of both ammonium and urea caused great changes in plant length, auxin contents and physiological measurements. Interesting correlations were found between the shoot auxin pool and both plant length and the “performance index” parameter, which is obtained from measurements of the kinetics of chlorophyll a fluorescence. Taken together, these data demonstrate that both the indole-3-acetic acid pool and performance index are important components of the response of M. truncatula under ammonium or urea as the sole N source.

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“…In bacteria, there are three general classes of SODs, which differ in their metal co-factors: the manganese-containing MnSOD (SOD A), the Fe-containing FeSOD (SOD B) and the cambialistic Fe/Mn SODs (CamSOD); the three SODs share high protein sequence and structure similarity (2, 3).…”

Section: Introductionmentioning

confidence: 99%

Increased oxidative stress tolerance by Superoxide dismutase overexpression inMesorhizobium loti

González¹,

Lozano

Lascano³

et al. 2018

Preprint

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Drought and salinity conditions are the major factors affecting nitrogen fixation by legume-rhizobium symbiosis. A global response to these stress conditions is the increase of intracellular ROS leading to activation of antioxidant system to ensure cellular homeostasis. Under the hypothesis that overexpression of sod gene in M. loti improve tolerance to oxidative stress we decide to investigate the response of constitutive overexpression of the Mesorhizobium loti MAFF303099 sod gene on tolerance to oxidative stress under free-living conditions. The gene mlr7636 was overexpressed constitutively under the nptII promoter of pFAJ1708 plasmid. Our study revealed that the sod overexpressing mutant had five-fold increased SOD activity in periplasmic space and a better tolerance to superoxide and hydrogen peroxide in bacterial killing assays. IMPORTANCE In this study, we report that the homologous superoxide dismutase overexpression in Mesorhizobium loti improves its tolerance to oxidative stress under free-living conditions.

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Expression and Localization of a Rhizobium-Derived Cambialistic Superoxide Dismutase in Pea (Pisum sativum) Nodules Subjected to Oxidative Stress

Cited by 14 publications

References 42 publications

Drought tolerance response of high‐yielding soybean varieties to mild drought: physiological and photochemical adjustments

Drought tolerance response of high‐yielding soybean varieties to mild drought: physiological and photochemical adjustments

Both Free Indole-3-Acetic Acid and Photosynthetic Performance are Important Players in the Response of Medicago truncatula to Urea and Ammonium Nutrition Under Axenic Conditions

Increased oxidative stress tolerance by Superoxide dismutase overexpression inMesorhizobium loti

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