Expression and localisation of calbindin D28k in all intestinal segments of the laying hen

Sugiyama, Toshie; Kikuchi, Hiroshi; Hiyama, Shinji; Nishizawa, Kazuya; Kusuhara, Seiji

doi:10.1080/00071660701302270

Cited by 27 publications

(18 citation statements)

References 30 publications

(38 reference statements)

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“…This result is in line with previous reports that immunoreactive CaBP-D28k was localised in the enterocyte cytoplasm of all intestinal segments in laying hen (Sugiyama et al, 2007). Thus, it is concluded that TRPV6 is expressed strongly in all segments of the intestine of laying hens, and active Ca 2+ transcellular transport may exert significant effects in Ca 2+ absorption from the intestinal lumen during the peak egg-laying stage.…”

Section: Discussionsupporting

confidence: 82%

Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens

Yang

Hou

Farquharson

et al. 2011

British Poultry Science

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In mammalian intestine, the passive entry of Ca 2+ into enterocytes via the transient receptor potential vanilloid channel type 6 (TRPV6) is recognized to be an important rate-limiting entry step in maintaining Ca 2+ homeostasis. There is, however, little information on the expression patterns of TRPV6 in the laying hen and therefore this study investigated TRPV6 localization and expression in different intestinal segments and kidney of laying hens during peak lay. Immunohistochemical analysis of the intestine indicated that TRPV6 was localized to the brush-border membranes of the duodenum, jejunum, ileum, cecum, and rectum. Expression was weaker in the rectum and little or no expression was found in crypt and goblet cells. In addition, TRPV6 mRNA was quantified amongst different intestinal segments and expression was highest in the duodenum and jejunum. Furthermore, western blotting indicated that the duodenum expressed the greatest amount of TRPV6 and the rectum the least with the other segments expressing intermediate levels. In the kidney, distinct immunopositive staining for TRPV6 was detected at the apical domain of the distal convoluted tubules (DCT) and medullary connecting tubules (CNT). Interestingly, distribution of TRPV6 extended to the proximal convoluted tubules (PCT), which is not generally implicated in active Ca 2+ transcellular reabsorption. Furthermore, the kidney expressed lower TRPV6 mRNA (P<0.05) and protein levels compared to duodenum possibly implicating an important role for the TRPV5 homolog in the kidney. In conclusion, the epithelial Ca 2+ channel TRPV6 is strongly expressed in the apical cells of the entire intestine and the renal tubules suggesting that active Ca 2+ transcellular transport plays a crucial role in dietary calcium (re)absorption in laying hens.

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Section: Discussionsupporting

confidence: 82%

Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens

Yang

Hou

Farquharson

et al. 2011

British Poultry Science

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“…Anti-calbindin-k28D positivity in duodenal intestine epithelium corroborates calcium absorption in this region. The greater intensity of positivity in the apical portion of enterocytes corroborates previous studies [41] that cite calbindin-k28D as a cellular calcium transport. This transporter binds to calcium absorbed by the cell and diffuses it into the cytoplasm, which is finally extruded by CA2 + -ATPase into the basolateral membrane, reaching the vascular system through lamina propria vessels [41].…”

Section: Resultssupporting

confidence: 90%

“…In contrast, goblet cells were not positive for anti-calbindin-k28D. These results corroborate the study carried out in layers, which states that in the intestine of layers, there is calbindin-D28k positivity (protein) in all segments, higher in the duodenum and jejunum, especially in the apical portions of the villi, but smaller in the ileum [41, 47].…”

Section: Resultssupporting

confidence: 88%

“…In modern strains of laying hens, which can be extrapolated to laying quails, the equivalent of 10% of total body calcium is transferred daily for deposition as eggshells [39–41]; the major sources of calcium are through absorption from the diet at the intestinal level, renal resorption, and bone storage. Since calbindin-k28D is the carrier responsible for the absorption of calcium from the digestive system, it would have the ability to modulate its deposition in the womb [46], in addition to intestinal absorptive capacity [45], influencing the production and the eggshell quality.…”

Section: Resultsmentioning

confidence: 99%

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Effects of methionine supplementing on intestine, liver and uterus morphology, and on positivity and expression of calbindin-D28k and TRPV6 calcium carriers in laying quails in thermoneutral conditions and under thermal stress

Moraes

Delicato

Cruz

et al. 2020

Preprint

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23The aim of this study was to provide support for the performance, localization and expression of the 24 epithelial calcium transporter channels, calbindin-D28k (Calb) and TRPV6, and of the morphology of the 25 digestive and reproductive system of laying quails under heat stress, and with methionine 26 supplementation. Therefore, the present study characterized the positivity (immunohistochemistry) and 27 expression (real-time PCR) of calcium channels (Calb and TRPV6) in the kidneys, intestine and uterus of 28 504 laying quails that were submitted to different methionine supplementation (100, 110 and 120%) and 29 temperatures (20, 24, 28 and 32ºC). The animals under thermal stress had lower villus height, villus:crypt 30 ratio, and goblet cell index in the duodenum and jejunum, fewer secondary and tertiary uterine folds, 31 smaller hepatic steatosis, and increased number of distal convoluted renal tubules (CT) positive to Calb 32 (protein), and increased positivity in proximal CTs. The deleterious effects of heat stress were minimized 33 with methionine supplementation for the following variables: duodenal crypts, number of goblet cells of 34 the jejunum, number of uterine folds, decreased Calb positivity in intestines and kidney, increased 35 positivity of Calb in the uterus and increased TRPV6 gene expression in the kidney. Calcium transporters 36 were altered due to less need for calcium absorption and reabsorption due to more calcium available with 37 the supplementation, increasing egg production and quality. Methionine supplementation further 38 increased intestinal villus absorption area and height, increased steatosis, decreased Calb positivity in the 39 intestine and kidney, increased uterine positivity and Calb expression, and increased TRPV6 expression 40 in the uterus under thermoneutrality. This is the first study that describes the gene and protein expression 41 of calcium transporters in the intestine, kidney and uterus of laying quails, and concludes that the use of 42 methionine supplementation is justifiable in order to partially reverse the deleterious effects of thermal 43 stress on the production. 44 45 46 3 47 65Methionine, classified as an essential amino acid [9], is also the first limiting factor in poultry 66 feed, and is essential for the maintenance, growth, production, and development of feathers [10]. In 67 addition to the productive responses obtained with methionine supplementation in laying hens, studies of 68 the morphological analyses of the laying digestive and reproductive system of broilers and light birds 69 indicate favorable quantitative and qualitative changes, such as an increase in egg mass by 10%. [11,12], 70 decreased laying fat [13], increased eggshell thickness [14], and increased intestinal villi [15], which 71 provide and technically justify the improvement in zootechnical indexes of these animals with methionine 72 use [15][16][17][18]. 73Evaluating the existing literature [15][16][17][18], diets supplemented with methionine at levels above 74 NRC (Nat...

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“…Layers actively absorb dietary Ca for eggshell formation along the entire gastrointestinal tract (Sugiyama et al, 2007). Whereas absolute Ca retention increases with increasing dietary Ca levels, the efficiency of the absorption process is inversely related to dietary Ca levels (Chandramoni & Sinha, 1998).…”

Section: Introductionmentioning

confidence: 99%

Nutrient balance of layers fed diets with different calcium levels and the inclusion of phytase and/or sodium butyrate

Vieira

Kessler

Ribeiro

et al. 2011

Rev. Bras. Cienc. Avic.

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In this study, Hisex Brown layers in lay were evaluated between 40 and 44 weeks of age to evaluate the inclusion of bacterial phytase (Ph) and sodium butyrate (SB) to diets containing different calcium levels (CaL). Performance, average egg weight and eggshell percentage, in addition to nutrient metabolizability and Ca and P balance were evaluated for 28 days. Birds were distributed according to a completely randomized experimental design with a 3x2x2 factorial arrangement, with three calcium levels (2.8, 3.3, 3.8%); the addition or not of phytase (500PhU/kg) and the addition or not of sodium butyrate (20mEq/kg), composing 12 treatments with eight replicates of one bird each. There was no additive effect of phytase or SB on the evaluated responses. Feed intake and feed conversion ratio were influenced by CaL, with the best performance obtained with 3.3% dietary Ca. Ca balance was positively affected by dietary Ca, and P balance by the addition of phytase. Ca dietary concentration, estimated to obtain Ca body balance, was 3.41%, corresponding to an apparent retention of 59.9% of Ca intake.

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Expression and localisation of calbindin D28k in all intestinal segments of the laying hen

Cited by 27 publications

References 30 publications

Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens

Localisation and expression of TRPV6 in all intestinal segments and kidney of laying hens

Effects of methionine supplementing on intestine, liver and uterus morphology, and on positivity and expression of calbindin-D28k and TRPV6 calcium carriers in laying quails in thermoneutral conditions and under thermal stress

Nutrient balance of layers fed diets with different calcium levels and the inclusion of phytase and/or sodium butyrate

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