Expression and its clinical significance of heat shock protein gp96 in human osteosarcoma

Wc, Guo; Sh, Zhao; Yu, Lan; Tang, Jing; Gx, Wu; Jl, Chen

doi:10.4149/neo_2010_01_062

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…6E , Fig. S6A, B , both super-resolution immunofluorescence imaging and analysis of subcellular fractions showed that gp96 is mainly localized in the ER, and a small fraction of gp96 also exists in the cytosol and nucleus, which was consistent to previous studies 34 , 35 . While Eomes and Trim28 mainly exist within the nucleus, small amounts of them were found to be distributed in the cytosol.…”

Section: Resultssupporting

confidence: 91%

Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes

Xu,

Li,

Cheng

et al. 2024

Nat Commun

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The maturation process of natural killer (NK) cells, which is regulated by multiple transcription factors, determines their functionality, but few checkpoints specifically targeting this process have been thoroughly studied. Here we show that NK-specific deficiency of glucose-regulated protein 94 (gp96) leads to decreased maturation of NK cells in mice. These gp96-deficient NK cells exhibit undermined activation, cytotoxicity and IFN-γ production upon stimulation, as well as weakened responses to IL-15 for NK cell maturation, in vitro. In vivo, NK-specific gp96-deficient mice show increased tumor growth. Mechanistically, we identify Eomes as the downstream transcription factor, with gp96 binding to Trim28 to prevent Trim28-mediated ubiquitination and degradation of Eomes. Our study thus suggests the gp96-Trim28-Eomes axis to be an important regulator for NK cell maturation and cancer surveillance in mice.

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Section: Resultssupporting

confidence: 91%

Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes

Xu,

Li,

Cheng

et al. 2024

Nat Commun

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“…It is also consistent with recent studies of esophageal squamous cell carcinomas [22], primary lung cancer [23] and osteosarcoma [24], which showed a correlation between Gp96 expression and prognosis of those carcinomas, suggesting that tumors with high Gp96 expression are very likely to recur and have a poor prognosis. However, the mechanism by which Gp96 expression contributes to tumor progression of NPC remains to be elucidated.…”

Section: Discussionsupporting

confidence: 80%

Upregulation of Gp96 Correlates with the Radiosensitivity and Five-Year Survival Rate of Nasopharyngeal Carcinoma

Zhou

Xiao²,

Yi³

et al. 2012

ORL

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Gp96 (GRP94) is a chaperone that is responsible for molecular folding and assembly of proteins. To investigate correlations among Gp96 expression, sensitivity to radiotherapy and clinical features of nasopharyngeal carcinoma (NPC), the expression of Gp96 in 98 NPC samples was analyzed by immunohistochemistry and Western blotting at the protein level, and by real-time PCR at the mRNA level. Clinicopathological features, including stage, sex, grade and survival, were compared between the high and low Gp96 expression groups. As a result, Gp96 was expressed at a higher level in NPC tissues than in noncancerous nasopharyngeal mucosa (p = 0.001). Univariate analysis suggested that a higher Gp96 expression level was associated with significantly decreased disease-free and 5-year survival rates (p = 0.000, p = 0.000, respectively). Multivariate Cox proportional-hazard regression analysis, which adjusts for clinicopathological variables, indicated that it was only associated with the 5-year overall survival rate (p = 0.002). Furthermore, patients with high Gp96 expression levels were significantly more resistant to radiotherapy (p = 0.001). These data are the first indication of a positive correlation between Gp96 expression levels and radiosensitivity and 5-year survival rate. Gp96 might serve as a novel candidate marker for predicting the long-term prognostic outcome of NPC.

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“…HSPgp96, was mainly expressed in the cytoplasm of osteoblastic sarcoma with less differentiation. The authors affirm that the HSPgp96 has a role in the pathogenesis of bone tumor, but the marker cannot be used in determining the degree of malignancy and as a target for tumor immunity (Guo et al, 2010). …”

Section: Osteosarcomamentioning

confidence: 99%

“…Guo et al (2010) assesses the role of heat shock protein gp96 (HSPgp96) in human osteosarcoma. HSPgp96, was mainly expressed in the cytoplasm of osteoblastic sarcoma with less differentiation.…”

Section: Osteosarcomamentioning

confidence: 99%

Biomarkers of Osteosarcoma, Chondrosarcoma, and Ewing Sarcoma

et al. 2017

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Osteosarcoma is the most frequent malignant bone neoplasm, followed by chondrosarcoma and Ewing sarcoma. The diagnosis of bone neoplasms is generally made through histological evaluation of a biopsy. Clinical and radiological features are also important in aiding diagnosis and to complete the staging of bone cancer. In addition to these, there are several non-specific serological or specific molecular markers for bone neoplasms. In bone tumors, molecular markers increase the accuracy of the diagnosis and assist in subtyping bone tumors. Here, we review these markers and discuss their role in the diagnosis and prognosis of the three most frequent malignant bone neoplasms, namely osteosarcoma, chondrosarcoma, and Ewing sarcoma.

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Expression and its clinical significance of heat shock protein gp96 in human osteosarcoma

Cited by 4 publications

References 18 publications

Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes

Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes

Upregulation of Gp96 Correlates with the Radiosensitivity and Five-Year Survival Rate of Nasopharyngeal Carcinoma

Biomarkers of Osteosarcoma, Chondrosarcoma, and Ewing Sarcoma

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