Expression and interaction of the CBLs and CIPKs from immature seeds of kidney bean (Phaseolus vulgaris L.)

Hamada, Shigeki; Seiki, Yoshiko; Watanabe, Kazuhiro; Ozeki, Takashi; Matsui, Hirokazu; Ito, Hiroyuki

doi:10.1016/j.phytochem.2009.02.002

Cited by 12 publications

(9 citation statements)

References 43 publications

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“…The results showed that TaCIPK29 could interact with TaCBL1, TaCBL2, TaCBL3 and TaCBL4, but very weakly with TaCBL6 and TaCBL7. In addition, 3-ami-notriazole (3-AT), as an inhibitor of His synthetase, was often used to identify the strong interaction through inhibiting the weak interaction of the pray and the bait [33]. When we supplied the selective medium with 10 mM 3-AT and 20 µM X-α-gal, we found that only the yeast cell with the AD-TaCIPK29 and BD-TaCBL2 or AD-TaCIPK29 and BD-TaCBL3 grew and exhibited blue color.…”

Section: Resultsmentioning

confidence: 99%

TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

et al. 2013

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Calcineurin B-like protein-interacting protein kinases (CIPKs) have been found to be responsive to abiotic stress. However, their precise functions and the related molecular mechanisms in abiotic stress tolerance are not completely understood, especially in wheat. In the present study, TaCIPK29 was identified as a new member of CIPK gene family in wheat. TaCIPK29 transcript increased after NaCl, cold, methyl viologen (MV), abscisic acid (ABA) and ethylene treatments. Over-expression of TaCIPK29 in tobacco resulted in increased salt tolerance, which was demonstrated by higher germination rates, longer root lengths and better growth status of transgenic tobacco plants compared to controls when both were treated with salt stress. Physiological measurements indicated that transgenic tobacco seedlings retained high K+/Na+ ratios and Ca2+ content by up-regulating some transporter genes expression and also possessed lower H2O2 levels and reduced membrane injury by increasing the expression and activities of catalase (CAT) and peroxidase (POD) under salt stress. Moreover, transgenic lines conferred tolerance to oxidative stress by increasing the activity and expression of CAT. Finally, TaCIPK29 was located throughout cells and it preferentially interacted with TaCBL2, TaCBL3, NtCBL2, NtCBL3 and NtCAT1. Taken together, our results showed that TaCIPK29 functions as a positive factor under salt stress and is involved in regulating cations and reactive oxygen species (ROS) homeostasis.

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Section: Resultsmentioning

confidence: 99%

TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

et al. 2013

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“…; Yang et al, 2008;Piao et al, 2010;Zhang et al, 2011Zhang et al, , 2013Chen et al, 2012;Wang et al, 2012;Deng et al, 2013;He et al, 2013). Moreover, lately, five novel CIPKs and two CBLs have been discovered in kidney bean (Phaseolus vulgaris) and 43 putative CIPK genes that are closely related to rice CIPKs have been identified in maize (Zea mays; Hamada et al, 2009;Chen et al, 2011). A function of a rice CIPK has been reported for OsCIPK31, which was found to modulate responses to abiotic stresses during seed germination and seedling growth (Piao et al, 2010), and OsCIPK23, which turned out to be a multistress-induced gene likely regulating signaling pathways during pollination and drought stress responses (Yang et al, 2008).…”

Section: The Cbl-cipk Network Is Conserved In Many Plant Speciesmentioning

confidence: 99%

Calcium and Reactive Oxygen Species Rule the Waves of Signaling

2013

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“…A recent study identified two CBL proteins from kidney bean ( Phaseolus vulgaris ) that are expressed in seeds. However, their function has not yet been determined (Hamada et al ., ). Other studies have reported that the calcium‐dependent protein kinases CDPK2 and CDPK11 in rice ( Oryza sativa , Frattini et al ., ) and two CDPK homologs in sandalwood ( Santalum album ; Anil et al ., ) are expressed in seeds.…”

Section: Introductionmentioning

confidence: 97%

The vacuolar calcium sensors CBL2 and CBL3 affect seed size and embryonic development in Arabidopsis thaliana

et al. 2014

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SUMMARYStimulus-specific calcium (Ca 2+ ) signals have crucial functions in developmental processes in many organisms, and are deciphered by various Ca 2+ -binding proteins. In Arabidopsis thaliana, a signaling network consisting of calcineurin B-like (CBL) protein calcium sensors and CBL-interacting protein kinases (CIPKs) has been shown to fulfil pivotal functions at the plasma membrane in regulating ion fluxes and abiotic stress responses. However, the role of tonoplast-localized CBL proteins and especially their function in regulating developmental programs remains largely unknown. In this study, we analyzed single and double mutants of the closely related tonoplast-localized calcium sensors CBL2 and CBL3, which show either reduction of function (rf) or complete loss of function (lf). While single cbl2 or cbl3 mutants did not display discernable phenotypes, cbl2/cbl3 mutants exhibited defects in vegetative growth and were severely impaired in seed development and morphology. Seeds of the cbl2/3rf mutant were smaller in size and exhibited reduced weight and fatty acid content compared to wild-type, but accumulation of sucrose was not altered. Moreover, accumulation of inositol hexakisphosphate (InsP 6 ), the major storage form of phosphorus in seeds, was significantly reduced in mutant seeds. In addition, complete loss of CBL2 and CBL3 function in cbl2/3lf resulted in a high frequency of severe defects in embryonic development. Together, our findings reveal a crucial function of Ca 2+ -controlled processes at the vacuolar membrane as determinants of seed yield and size, and demonstrate the importance of vacuolar CBL calcium sensors for plant embryogenesis.

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Expression and interaction of the CBLs and CIPKs from immature seeds of kidney bean (Phaseolus vulgaris L.)

Cited by 12 publications

References 43 publications

TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

Calcium and Reactive Oxygen Species Rule the Waves of Signaling

The vacuolar calcium sensors CBL2 and CBL3 affect seed size and embryonic development in Arabidopsis thaliana

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