Expression and immunohistochemical detection of Nesfatin-1 in the gastrointestinal tract of Casertana pig

Varricchio, Ettore; Russolillo, M; Russo, Finizia; Lombardi, Vittoria; Paolucci, Marina; Maruccio, Lucianna

doi:10.1016/j.acthis.2013.11.006

Cited by 16 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IR cells for nesfatin-1, ghrelin and leptin were observed in the villi, crypts and ENS, in both the duodenum and jejunum, with IR cells for VIP observed in the ENS. These results are partly consistent with the results of previous studies in relation to the location of these hormones in the intestine [55,61,62,[72][73][74]. We observed a decrease in the expression of nesfatin-1 and leptin in the duodenum mucosa and a decrease in ghrelin expression in the jejunum mucosa.…”

Section: Discussionsupporting

confidence: 93%

Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

Puzio

Muszyński

Dobrowolski

et al. 2021

JCM

View full text Add to dashboard Cite

The stomach is responsible for the processing of nutrients as well as for the secretion of various hormones which are involved in many activities throughout the gastrointestinal tract. Experimental adult male Wistar rats (n = 6) underwent a modified gastrectomy, while control rats (n = 6) were sham-operated. After six weeks, changes in small intestine (including histomorphometrical parameters of the enteric nervous plexuses) and liver morphology, immunolocalization of leptin, ghrelin and nesfatin-1 as well as proteins forming adherens and tight junctions (E-cadherin, zonula occludens-1, occludin, marvelD3) in intestinal mucosa were evaluated. A number of effects on small intestine morphology, enteric nervous system ganglia, hormones and proteins expression were found, showing intestinal enteroplasticity and neuroplasticity associated with changes in gastrointestinal tract condition. The functional changes in intestinal mucosa and the enteric nervous system could be responsible for the altered intestinal barrier and hormonal responses following gastrectomy. The results suggest that more complicated regulatory mechanisms than that of compensatory mucosal hypertrophy alone are involved.

show abstract

Section: Discussionsupporting

confidence: 93%

Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

Puzio

Muszyński

Dobrowolski

et al. 2021

JCM

View full text Add to dashboard Cite

show abstract

“…Post-translational processing by prohormone convertases (PC 1/3 and PC 2) causes NUCB2 to be cleaved into three peptides, nesfatin-1 (1–82 amino acids), nesfatin-2 (85–163 amino acids), and nesfatin-3 (166–396 amino acids). NUCB2/nesfatin-1 amino acid sequence is highly conserved across vertebrates [6] , [7] , [31] . NUCB2/nesfatin-1 is found in various hypothalamic nuclei that are involved in energy metabolism, such as the arcuate nucleus, paraventricular nucleus, supraoptic nucleus, lateral hypothalamic area and zona increta [8] , [9] .…”

Section: Introductionmentioning

confidence: 99%

Nutrients Differentially Regulate Nucleobindin-2/Nesfatin-1 In Vitro in Cultured Stomach Ghrelinoma (MGN3-1) Cells and In Vivo in Male Mice

et al. 2014

View full text Add to dashboard Cite

Nesfatin-1 is secreted, meal-responsive anorexigenic peptide encoded in the precursor nucleobindin-2 [NUCB2]. Circulating nesfatin-1 increases post-prandially, but the dietary components that modulate NUCB2/nesfatin-1 remain unknown. We hypothesized that carbohydrate, fat and protein differentially regulate tissue specific expression of nesfatin-1. NUCB2, prohormone convertases and nesfatin-1 were detected in mouse stomach ghrelinoma [MGN3-1] cells. NUCB2 mRNA and protein were also detected in mouse liver, and small and large intestines. MGN3-1 cells were treated with glucose, fatty acids or amino acids. Male C57BL/6 mice were chronically fed high fat, high carbohydrate and high protein diets for 17 weeks. Quantitative PCR and nesfatin-1 assays were used to determine nesfatin-1 at mRNA and protein levels. Glucose stimulated NUCB2 mRNA expression in MGN3-1 cells. L-Tryptophan also increased NUCB2 mRNA expression and ghrelin mRNA expression, and nesfatin-1 secretion. Oleic acid inhibited NUCB2 mRNA expression, while ghrelin mRNA expression and secretion was enhanced. NUCB2 mRNA expression was significantly lower in the liver of mice fed a high protein diet compared to mice fed other diets. Chronic intake of high fat diet caused a significant reduction in NUCB2 mRNA in the stomach, while high protein and high fat diet caused similar suppression of NUCB2 mRNA in the large intestine. No differences in serum nesfatin-1 levels were found in mice at 7 a.m, at the commencement of the light phase. High carbohydrate diet fed mice showed significantly elevated nesfatin-1 levels at 1 p.m. Serum nesfatin-1 was significantly lower in mice fed high fat, protein or carbohydrate compared to the controls at 7 p.m, just prior to the dark phase. Mice that received a bolus of high fat had significantly elevated nesfatin-1/NUCB2 at all time points tested post-gavage, compared to control mice and mice fed other diets. Our results for the first time indicate that nesfatin-1 is modulated by nutrients.

show abstract

“…Nesfatin-1 immunoreactivity has been reported in Brunner's glands of the duodenum and in the other parts of small and large intestines in rats and mice (19,21,22,29). In pigs, nesfatin-1 immunoreactivity was described in the entire the gastrointestinal tract with localization in the endocrine cells of the gastric fundus, duodenum, ileum, ileocecal valve, and colon (25). In dogs, nesfatin-1 was only detected in the stomach and pancreas, but nesfatin-1 mRNA was detected in the entire gastrointestinal tract (13).…”

Section: Resultsmentioning

confidence: 99%

Immunohistochemical localization of adropin in the small intestine of rats

Pawłowska-Olszewska¹,

Puzio²,

Kapica³

et al. 2019

Medycyna Weterynaryjna

View full text Add to dashboard Cite

The aim of this study was to determine the occurrence and distribution of adropin in the small intestine in control and gastrectomized rats. The study was carried out on 12 Wistar rats. Six control rats (SHO) underwent a sham operation. Six rats were subjected to gastrectomy (Gx); i.e. resection of the antrum and the glandular part of the stomach. Six weeks after the surgery, the rats were sacrificed. The duodenum and middle part of the jejunum were collected for immunohistochemical and immunofluorescence procedures. An immunopositive reaction to ADR was detected in the duodenum and jejunum, in crypts, and in enterocytes located along the entire length of the villi. Furthermore, more intense ADR immunoreactivity was observed in the crypts and villi of the duodenum than in the jejunum. The presence of adropin was also detected in the submucosa and muscularis externa of the duodenum and jejunum, in the cells of Brunner’s gland of the duodenum, and in myenteric and submucosal plexus nerve. The ADR immunoreactivity in the crypts and villi in the duodenum and jejunum was lower in the Gx rats compared to the SHO rats. The ADR distribution in the duodenum and jejunum has also been confirmed in immunofluorescence studies. In conclusion, our data demonstrated ADR expression in all histological layers of the small intestine in the rats. Gx reduced ADR immunoreactivity in the crypts and enterocytes. This may suggest a potential impact of ADR on intestine function such as absorbability and immune reactions. However, further research is needed to explain the ADR role in gastrointestinal functions

show abstract

Expression and immunohistochemical detection of Nesfatin-1 in the gastrointestinal tract of Casertana pig

Cited by 16 publications

References 32 publications

Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

Nutrients Differentially Regulate Nucleobindin-2/Nesfatin-1 In Vitro in Cultured Stomach Ghrelinoma (MGN3-1) Cells and In Vivo in Male Mice

Immunohistochemical localization of adropin in the small intestine of rats

Contact Info

Product

Resources

About