Expression and functional characterization of transferrin in Nile tilapia (Oreochromis niloticus) in response to bacterial infection

Yin, Xiaoxue; Mu, Liangliang; Bian, Xia; Wu, Liting; Li, Bingxi; Liu, Jing; Guo, Zheng; Ye, Jianmin

doi:10.1016/j.fsi.2018.01.023

Cited by 54 publications

(26 citation statements)

References 49 publications

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“…Transferrin is an iron-binding glycoprotein involved in the transport and storage of iron, it participates in the regulation of breathing, cell proliferation and differentiation, and also plays an important role in host defence against pathogenic infection, not only because it inhibits the growth and proliferation of pathogens, but it also activates anti-microbial responses in macrophages [72]. Similarly, the expression of transferrin was significantly up-regulated in other fish species exposed to bacterial [73] and parasitic [74] pathogens, in the latter case also in gills. The higher expression of transferrin in P gills could correlate with a higher need of iron to transport the low oxygen available or alternatively it could be an effort to increase iron storage to deprive its availability for the parasite.…”

Section: Discussionmentioning

confidence: 99%

Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

et al. 2019

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Background: Monogenean flatworms are the main fish ectoparasites inflicting serious economic losses in aquaculture. The polyopisthocotylean Sparicotyle chrysophrii parasitizes the gills of gilthead sea bream (GSB, Sparus aurata) causing anaemia, lamellae fusion and sloughing of epithelial cells, with the consequent hypoxia, emaciation, lethargy and mortality. Currently no preventive or curative measures against this disease exist and therefore information on the hostparasite interaction is crucial to find mitigation solutions for sparicotylosis. The knowledge about gene regulation in monogenean-host models mostly comes from freshwater monopysthocotyleans and almost nothing is known about polyopisthocotyleans. The current study aims to decipher the host response at local (gills) and systemic (spleen, liver) levels in farmed GSB with a mild natural S. chrysophrii infection by transcriptomic analysis. Results: Using Illumina RNA sequencing and transcriptomic analysis, a total of 2581 differentially expressed transcripts were identified in infected fish when compared to uninfected controls. Gill tissues in contact with the parasite (P gills) displayed regulation of fewer genes (700) than gill portions not in contact with the parasite (NP gills) (1235), most likely due to a local silencing effect of the parasite. The systemic reaction in the spleen was much higher than that at the parasite attachment site (local) (1240), and higher than in liver (334). NP gills displayed a strong enrichment of genes mainly related to immune response and apoptosis. Processes such as apoptosis, inflammation and cell proliferation dominated gills, whereas inhibition of apoptosis, autophagy, platelet activation, signalling and aggregation, and inflammasome were observed in spleen. Proteasome markers were increased in all tissues, whereas hypoxia-related genes were down-regulated in gills and spleen. Conclusions: Contrasting forces seem to be acting at local and systemic levels. The splenic down-regulation could be part of a hypometabolic response, to counteract the hypoxia induced by the parasite damage to the gills and to concentrate the energy on defence and repair responses. Alternatively, it can be also interpreted as the often observed action of helminths to modify host immunity in its own interest. These results provide the first toolkit for future studies towards understanding and management of this parasitosis.

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Section: Discussionmentioning

confidence: 99%

Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

et al. 2019

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“…Nile tilapia, about 80 ± 10 g (for challenge experiment) and 300 ± 20 g (for cell separation) were procured from the Guangdong Tilapia Breeding Farm (Guangzhou, China), which were cultured as previously described ( 28 , 31 ). The Balb/c female mice for the preparation of polyclonal antibody (pAb) were purchased from Guangdong Medical Laboratory Animal Center (Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

“…To characterize the tissue distribution of OnMAp34 , the samples from healthy O. niloticus were collected, including liver, head kidney, hind kidney, spleen, skin, gills, peripheral blood, thymus, muscle, and intestine. To explore the variation of OnMAp34 after bacterial challenges, the experimental fish were intraperitoneally injected with 0.1 mL live S. agalactiae (ZQ0910, 1 × 10 7 CFU/mL) or A. hydrophila (BYK00810, 1 × 10 7 CFU/mL) ( 28 , 31 ). The control group was stimulated with 0.1 mL sterilized phosphate-buffered saline (PBS, 10 mM phosphate, 150 mM NaCl, pH 7.4) per fish.…”

Section: Methodsmentioning

confidence: 99%

MAp34 Regulates the Non-specific Cell Immunity of Monocytes/Macrophages and Inhibits the Lectin Pathway of Complement Activation in a Teleost Fish

Yin

et al. 2020

Front. Immunol.

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The lectin pathway of the complement system is one of the main components of innate immunity, which plays a pivotal role in the defense against infectious microorganisms and maintains immune homeostasis. However, its control mechanisms remain unclear in teleost fish. In this study, we described the identification and functional characterization of a mannose-binding lectin associated protein MAp34 (OnMAp34) from Nile tilapia ( Oreochromis niloticus ) at molecular, cellular, and protein levels. The open reading frame (ORF) of OnMAp34 is 918 bp of nucleotide sequence encoding a polypeptide of 305 amino acids. The deduced amino acid sequence has three characteristic structures, including two C1r/C1s-Uegf-BMP domains (CUB) and one epidermal growth factor domain (EGF). Expression analysis revealed that the OnMAp34 was highly expressed in the liver and widely existed in other examined tissues. In addition, the mRNA and protein expression levels of OnMAp34 were remarkably altered upon infection with Streptococcus agalactiae and Aeromonas hydrophila in vivo and in vitro . Further, we found that the OnMAp34 could participate in the non-specific cellular immune defense, including the regulation of inflammation, migration, and enhancement of phagocytosis of monocytes/macrophages. Moreover, the OnMAp34 could compete with OnMASPs to combine OnMBL and inhibit the lectin pathway of complement activation. Overall, our results provide new insights into the understanding of MAp34 as a potent regulator in the lectin complement pathway and non-specific cell immunity in an early vertebrate.

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“…The cells were cultured in 96‐well plate (Corning, USA; 100 μl/well) with the final concentration of 1 × 10 6 cells/ml per well and then stimulated with poly (I:C) (50 μg/ml), S. agalactiae (1 × 10 7 CFU/ml) (Mu et al, ; Wang, Seymour, Sundberg, & Hogenesch, ) and A. hydrophila (1 × 10 7 CFU/ml) at 25℃. Bacteria of S. agalactiae and A. hydrophila (Tang et al, ; Yin et al, ) were cultured and inactivated and control group with the equal volume of 1 × PBS as same as our previous study. Preparation and application of bacteria were the same as laboratory methods (Mu et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…agalactiae (1 × 10 7 CFU/ml) (Mu et al, 2017; and A. hydrophila (1 × 10 7 CFU/ml) at 25℃. Bacteria of S. agalactiae and A. hydrophila (Tang et al, 2014;Yin et al, 2018) were cultured and inactivated and control group with the equal volume of 1 × PBS as same as our previous study. Preparation and application of bacteria were the same as laboratory methods .…”

Section: Primersmentioning

confidence: 99%

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

Kong

et al. 2020

Aquac Res

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CD3 is an important membrane molecule of mature T cells. TCR‐CD3 complex plays important roles in the activation and signal transduction of T cells. In this study, the CD3ε (OnCD3ε) gene was cloned from Nile tilapia (Oreochromis niloticus), and its tissue distribution was detected by qPCR. The expression changes in CD3ε were analysed after three challenges (S. agalactiae, A. hydrophila and poly (I:C)) in vivo and in vitro. The open reading frame (ORF) of OnCD3ε contains 531 bp, encoding 176 amino acids, which found that OnCD3ε consists of conserved amino acid residues and motifs (cysteine residues, CXXC motif, immunoreceptor tyrosine‐based activation motifs and PxPDY). In addition, tissue distribution displayed that OnCD3ε has the highest expression in thymus and significant expression level in mucosal immune‐related tissues (intestine and gills). In vivo, all three challenges could significantly up‐regulate the expression of CD3ε in the head kidney and spleen. For further in vitro assays, after poly (I:C) stimulation, OnCD3ε appeared significant up‐regulation at 3 hr post stimulation, and two pathogenic bacteria also induce significant up‐regulation of OnCD3ε in spleen leucocytes (12 hr). These results suggested that OnCD3ε was likely to get involved in host immune response against pathogenic challenges.

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Expression and functional characterization of transferrin in Nile tilapia (Oreochromis niloticus) in response to bacterial infection

Cited by 54 publications

References 49 publications

Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

MAp34 Regulates the Non-specific Cell Immunity of Monocytes/Macrophages and Inhibits the Lectin Pathway of Complement Activation in a Teleost Fish

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

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