Expression and functional analysis of PhEOL1 and PhEOL2 during flower senescence in petunia

Abstract

The ethylene biosynthesis pathway controls flower senescence. Previous studies have shown that Arabidopsis ETHYLENE-OVERPRODUCER1 (ETO1) interacts specifically with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases (ACSs), the rate-limiting enzymes of ethylene biosynthesis. The ethylene biosynthesis pathway controls flower senescence in petunias (Petunia hybrida Juss.). However, the role of ETO1-like genes (EOLs) during flower senescence has not been investigated. Here, two full-length petunia EOL cDNAs, PhEOL1 and PhEOL2, were isolated. RT–PCR assays indicated that the expression of PhEOL1 and PhEOL2 increased after exogenous ethylene treatment. The VIGS-mediated silencing of PhEOL1 accelerated flower senescence and produced more ethylene than the control condition, whereas the silencing of PhEOL2 did not. Notably, the effects caused by PhEOL1 suppression were not enhanced by PhEOL2 suppression in corollas. In addition, the expression of two petunia type 2 PhACS genes increased during flower senescence and after ethylene treatment. A yeast two-hybrid assay showed that PhEOL1 interacts with both PhACS2 and PhACS3. It is possible that PhEOL1 is involved in flower senescence by interacting with type 2 PhACSs in petunias.

Ethylene Role in Plant Growth, Development and Senescence: Interaction with Other Phytohormones

“…ACS is the rate-limiting enzyme of ethylene biosynthesis in plants (Wang et al, 2002), and its activity regulation may involve post-transcriptional regulation through its degradation (Wang et al, 2004). In Arabidopsis the ETHYLENE-OVERPRODUCER1 -like ( EOL1 ), negatively regulates ethylene biosynthesis (Wang et al, 2004) and in petunia the VIGS-mediated suppression of PhEOL1 accelerated the senescence of flowers and increased ethylene production in corollas (Liu et al, 2016). …”

Comparative Transcriptome Analysis of Flower Senescence of Camellia lutchuensis

“…The phytohormone ethylene plays a key role in plant growth, development and senescence [ 33 ]. ACS and ACO are the key genes of ethylene synthesis, encoding enzymes that catalyze the synthesis of ethylene from methionine [ 34 , 35 ]. The accelerated induction of DcACS1 and DcACO1 genes during petal senescence in cut carnation flowers [ 20 , 36 ].…”