Expression and functional analysis of cathepsin L1 in ovarian development of the oriental river prawn, Macrobrachium nipponense

Zhu, Junpeng; Fu, Hongtuo; Qiao, Hui; Jin, Shubo; Zhang, Wenyi; Jiang, Sufei; Gong, Yongsheng; Xiong, Yiwei

doi:10.1016/j.aqrep.2021.100724

Cited by 15 publications

(18 citation statements)

References 41 publications

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“…Ovary stage III was a rapid period of growth and proliferation of oocytes, which provided nutrients and energy. Previous studies ( 42 , 43 ) suggested that two cathepsin L genes are mainly expressed in the hepatopancreas, and their expression levels in the ovary reached the maximum in stage III, which was similar to Mn-Lel. ISH results also showed that a clear Mn-Lel signal was detected in all five stages.…”

Section: Discussionmentioning

confidence: 60%

A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation

et al. 2022

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Legumain, also called aspartic endopeptidase (AEP), is a member of the cysteine protease family and is involved in various physiological processes. In this study, we analyzed the characteristics of a novel legumain-like (named Mn-Lel) in the female oriental river prawn, Macrobrachium nipponense, which is involved in ovary maturation. The Mn-Lel is 1,454 bp in length, including a 1,290-bp open reading frame that encodes 430 amino acids. qPCR analysis indicated that Mn-Lel is specifically highly expressed in the hepatopancreas and ovaries of female prawns. It is rarely expressed in embryogenesis, weakly expressed in early larval development stages, and then significantly increased after metamorphosis, which indicated that Mn-Lel is not a maternal gene and mainly plays a role in adults. During the different ovarian stages, Mn-Lel expression in the hepatopancreas had no obvious rules, while its expression in the ovaries had a significant peak in stage III. In situ hybridization studies revealed that Mn-Lel is localized in the oocyte of the ovary. Changes in the gonadosomatic index confirmed the inhibitory effects of Mn-Lel dsRNA on ovary maturation. These results suggest that Mn-Lel has a key role in promoting ovary maturation.

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Section: Discussionmentioning

confidence: 60%

A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation

et al. 2022

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“…The big difference was that Mn-CL2 found in this study, as well as Mn-CTS L1 (another cathepsin L detected in the ovary transcriptomes of M. nipponense form our lab) expressed highly in ovary. Especially, Mn-CL2 had rarely expression in other tissues except for hepatopancreas and ovary ( 4 ). These results gave evidence that Mn-CL2 play a special role in the ovaries.…”

Section: Discussionmentioning

confidence: 99%

“…From the transcriptomes of Stages I–V stages of ovary in M. nipponense , we characterized several cathepsin L genes which showed evidences that involve in ovary maturation ( 3 , 4 ) and more details about their roles in crustaceans needs to be clarified and demonstrated. In this paper, RNAi was used to illustrate the special roles of Mn-CL2 in ovary maturation of M. nipponense .…”

Section: Discussionmentioning

confidence: 99%

“…According to the comparison analysis of differentially expressed genes and KEGG enrichment in five stages of ovarian transcriptomes, several genes closely related to rapid ovary maturation were screened. The most significant enriched pathway in vitellogenesis stages was lysosomal pathway and we got several cathepsin L genes which might involve in ovary maturation from this pathway ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

et al. 2022

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Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.

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“…CtsL1 is an important member of the cysteine protease family and plays a crucial role in oocyte maturation [ 53 ]. In shrimp, Mn-CTS L1 has been verified to be involved in hydrolyzing vitellogenin before ovarian maturity to provide nutrients for the maturation of oocytes [ 54 ]. Furthermore, silencing cathepsin L using RNAi led to the accumulation of vitellogenin [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Insulin-like Peptide (ILP) and Its Potential Role in Ovarian Development of the Cuttlefish Sepiella japonica

Lü

Yao

Zhao

et al. 2022

CIMB

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The insulin-like peptide (ILP) family is well known for regulating reproduction in invertebrates, while its role in mollusks remains largely unknown. In this study, we first isolated and characterized the ILP gene in the cuttlefish Sepiella japonica. The full-length SjILP cDNA obtained was 926 bp and encoded a precursor protein of 161 amino acids. The precursor protein consisted of a signal peptide, a B chain, a C-peptide, and an A chain. It possessed the typical features of ILP proteins, including two cleavage sites (KR) and eight conserved cysteines. To define the function of SjILP, the expression of SjILP in different tissues and ovarian development stages were analyzed using qRT-PCR. SjILP was mainly expressed in the ovary, and its gene expression correlated with ovarian development. Furthermore, silencing SjILP using RNA interference (RNAi) dramatically decreased the expression levels of four ovarian-development-related genes (vitellogenin1, vitellogenin2, cathepsin L1-like, and follistatin). These data suggest the critical role of SjILP in the regulation of ovarian development in S. japonica.

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Expression and functional analysis of cathepsin L1 in ovarian development of the oriental river prawn, Macrobrachium nipponense

Cited by 15 publications

References 41 publications

A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation

A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation

Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation

Characterization of Insulin-like Peptide (ILP) and Its Potential Role in Ovarian Development of the Cuttlefish Sepiella japonica

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