Expression and functional analysis of a PR-1 Gene, <i>MuPR1</i>, involved in disease resistance response in mulberry (<i>Morus multicaulis</i>)

Fang, Lichun; Qin, Rong-Li; Liu, Zhuang; Liu, Chao-Rui; Gai, Yingping; Ji, Xiaodong

doi:10.1080/17429145.2019.1640295

Cited by 16 publications

(6 citation statements)

References 47 publications

(51 reference statements)

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“…qRT-PCR analysis revealed that the PtPRR7 gene was expressed in roots, stems, and leaves, whilst the PtPRR7 gene was the highest expression in roots. The results are in agreement with a study in mulberry (Fang et al, 2019).…”

Section: Conflict Of Interestssupporting

confidence: 92%

Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Ding

Huang

Shu

et al. 2020

Not Bot Horti Agrobo

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Circadian clock is usually involved in many physiological processes of plants, including responses to abiotic stress, whilst pseudo-response regulator 7 (PRR7) gene is the main component of the circadian clock. In this study, the cDNA of the PRR7 gene was obtained from trifoliate orange (Poncirus trifoliata). Based on the sequence analysis, the PtPRR7 gene had an open reading frame of 2343 bp, encoded 780 amino acids, and contained proteins of the REC and CCT domains. Subcellular localization indicated that PtPRR7 was mainly localized in the nucleus and a small amount of cytoplasm. qRT-PCR analysis revealed the highest expression level of PtPRR7 in roots than in both shoots and leaves. The PtPRR7 gene during 24 hours of soil water deficit exhibited a circadian rhythmic expression pattern: the expression peak at 9:00 am in leaves and at 21:00 pm in roots. Drought treatment affected PtPRR7 gene expression. Such data provide important references for understanding the characteristics of PtPRR7 gene in citrus plants.

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Section: Conflict Of Interestssupporting

confidence: 92%

Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Ding

Huang

Shu

et al. 2020

Not Bot Horti Agrobo

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“…However, the drawbacks of wood, such as dimensional instability when exposed to wet conditions and biological degradation when exposed to decay fungi, limit its long-term utilization [6][7][8][9][10]. Although its biodegradable nature is beneficial, from the perspective of sustainable development wood still needs to be modified to obtain better durability when exposed to the above harsh environments [11][12][13][14][15]. The durability of wood with respect to deformation in water and biological degradation due to decay fungi is essentially related to its cell wall components and microstructure [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

et al. 2020

Forests

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Wood is susceptible to swelling deformation and decay fungi due to moisture adsorption that originates from the dynamic nanopores of the cell wall and the abundant hydroxyl groups in wood components. This study employed as a modifier maleic anhydride (MAn), with the help of acetone as solvent, to diffuse into the wood cell wall, bulk nanopores, and further chemically bond to the hydroxyl groups of wood components, reducing the numbers of free hydroxyl groups and weakening the diffusion of water molecules into the wood cell wall. The derived MAn-bulked wood, compared to the control wood, presented a reduction in water absorptivity (RWA) of ~23% as well as an anti-swelling efficiency (ASE) of ~39% after immersion in water for 228 h, and showed an improvement in decay resistance of 81.42% against white-rot fungus and 69.79% against brown-rot fungus, respectively. The method of combined cell wall bulking and hydroxyl group bonding could effectively improve the dimensional stability and decay resistance with lower doses of modifier, providing a new strategy for wood durability improvement.

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“…DC3000 (Fang et al . 2019), as well as in response to leaf rust fungal infection (Gao et al . 2014).…”

Section: Discussionmentioning

confidence: 99%

Dynamics of reactive oxygen species and lignin biosynthesis during leaf spot disease of Withania somnifera (L.) Dunal

et al. 2023

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Withania somnifera is an important medicinal plant, however, its cultivation and quality are compromised through infestation by leaf spot disease caused by the fungus, Alternaria alternata. To find suitable strategies against this disease, studies on postinfectional changes are important. ROS are critical as they interact with other defence signalling pathways. We analyzed ROS-generating and scavenging systems in healthy and diseased leaf samples of W. somnifera and ROS-driven downstream defence pathways.• We used DAB and NBT assays for ROS detection, spectrophotometry and in-gel assays for ROS scavenging enzymes, a thioglycolic acid (TGA) based assay, histochemical staining for lignin, and qRT-PCR for transcript-level expression.• Leaf spot infection in W. somnifera increased NADPH oxidase activity and ROS accumulation in infected leaves, together with enhanced antioxidant enzyme activity. Leaf spot-infected leaves had increased lignin content and higher expression of lignin biosynthesis genes. In addition, transcript levels of defence-related genes, NPR1 and PR, were also upregulated.• The present work provides insights into responses to leaf spot disease through defencerelated signalling in W. somnifera. It demonstrates crosstalk between ROS and lignin biosynthesis. This work identified potential targets for developing strategies to confer disease resistance against A. alternata in W. somnifera.

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Expression and functional analysis of a PR-1 Gene, MuPR1, involved in disease resistance response in mulberry (Morus multicaulis)

Abstract: In the present study, the cDNA (designated MuPR1) of PR1 gene was obtained from mulberry. Our results showed that MuPR1 shares some conserved characteristics with its homologues from different plant families. MuPR1 was localized exclusively to the extracellular spaces, and the MuPR1

Cited by 16 publications

References 47 publications

Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

Dynamics of reactive oxygen species and lignin biosynthesis during leaf spot disease of Withania somnifera (L.) Dunal

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