Expression and Function of Tetraspanins and Their Interacting Partners in B Cells

Feng, Zhang‐Qi; Wang, Xu; Han, Xinxin; Rothschild, Gerson; Zheng, Song Guo; Basu, Uttiya; Sun, Jianbo

doi:10.3389/fimmu.2018.01606

Cited by 60 publications

(44 citation statements)

References 110 publications

(82 reference statements)

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“…cd63 is a known tetraspanin family member in rainbow trout (Castro et al, 2015) and cd44 has been reported as a tetraspanin associated protein in mammals (Zou et al, 2018) (Fig. 7).…”

Section: Inflammation Associated: Autophagy Apoptosis and Ecm Componmentioning

confidence: 99%

Transcriptomic analysis of the impacts of ethinylestradiol (EE2) and its consequences for proliferative kidney disease outcome in rainbow trout (Oncorhynchus mykiss)

Bailey

Siebenthal

Rehberger

et al. 2019

Comparative Biochemistry and Physiology Part C: Toxicology &

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Freshwater fish are threatened by the cumulative impact of multiple stressors. The purpose of this study was to unravel the molecular and organism level reactions of rainbow trout, Oncorhynchus mykiss, to the combined impact of two such stressors that occur in the natural habitat of salmonids. Fish were infected with either the myxozoan parasite, Tetracapsuloides bryosalmonae, which causes proliferative kidney disease (PKD), or exposed to ethinylestradiol (EE2) an estrogenic endocrine disrupting compound, or to a combination of both (PKD x EE2). PKD is a slow progressive chronic disease here we focused on a later time point (130-day postinfection (d.p.i)) when parasite intensity in the fish kidney has already started to decrease. At 130 d.p.i, RNA-seq technology was applied to the posterior kidney, the main target organ for parasite development. This resulted with 280 (PKD), 14 (EE2) and 444 (PKD x EE2) differentially expressed genes (DEGs) observed in the experimental groups. In fish exposed to the combination of stressors (PKD x EE2), a number of pathways were regulated that were neither observed in the single stressor groups. Parasite infection, alone and in combination with EE2, only resulted in a low intensity immune response that negatively correlated with an upregulation of genes involved in a variety of metabolic and inflammation resolution processes. This could indicate a trade-off whereby the host increases investment in recovery/resolution processes over immune responses at a later stage of disease. When PKD infection took place under simultaneous exposure to EE2 (PKD x EE2), parasite intensity decreased and pathological alterations in the posterior kidney were reduced in comparison to the PKD only condition. These findings suggest that EE2 modulated these response profiles in PKD infected fish, attenuating the disease impact on the fish.

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“…cd63 is a known tetraspanin family member in rainbow trout (Castro et al, 2015) and cd44 has been reported as a tetraspanin associated protein in mammals (Zou et al, 2018) (Fig. 7).…”

Section: Inflammation Associated: Autophagy Apoptosis and Ecm Componmentioning

confidence: 99%

Transcriptomic analysis of the impacts of ethinylestradiol (EE2) and its consequences for proliferative kidney disease outcome in rainbow trout (Oncorhynchus mykiss)

Bailey

Siebenthal

Rehberger

et al. 2019

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…CD86 is a co-stimulator of CD28-mediated T cell activation 24 . CD63 is a tetraspanin protein with numerous regulatory roles 25 , including exosomebased co-stimulation of T cells.…”

Section: Resultsmentioning

confidence: 99%

Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans

et al. 2020

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Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but very few studies have detected such effects in humans. Here, we analyzed single-cell expression of four proteins (CD23, CD55, CD63 and CD86) across cell lines derived from individuals of the Yoruba population. Using data from over 30 million cells, we found substantial inter-individual variation of dispersion. We demonstrate, via de novo cell line generation and subcloning experiments, that this variation exceeds the variation associated with cellular immortalization. We detected a genetic association between the expression dispersion of CD63 and the rs971 SNP. Our results show that human DNA variants can have inherently-probabilistic effects on gene expression. Such subtle genetic effects may participate to phenotypic variation and disease outcome.

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“…CD86 is a costimulator of CD28-mediated T-cell activation 27 . CD63 is a tetraspanin with numerous regulatory roles 28 , including exosome-based co-stimulation of T cells. Thus, different degrees of CD55, CD86 or CD63 expression dispersion in B cells may in turn generate heterogeneities among T cells.…”

Section: Discussionmentioning

confidence: 99%

Cell-to-cell expression dispersion of B-cell surface proteins displays genetic variation among humans

Triqueneaux

Burny

Symmons

et al. 2019

Preprint

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Ph: +33 4 72 72 87 17, Gael.Yvert@ens-lyon.fr 2 ABSTRACT Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but whether such effects exist in humans has not been fully demonstrated. Here, we analyzed single-cell expression of four proteins (CD23, CD55, CD63 and CD86) across cell lines derived from individuals of the Yoruba population. Using data from over 30 million cells, we found substantial interindividual variation of dispersion. We demonstrate, via de novo cell line generation and subcloning experiments, that this variation exceeds the variation associated with cellular immortalization. By association mapping, we linked the expression dispersion of CD63 to the rs971 SNP. Our results show that human DNA variants can have inherently-probabilistic effects on gene expression. Such subtle genetic effects may participate to phenotypic variation and disease predisposition.

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Expression and Function of Tetraspanins and Their Interacting Partners in B Cells

Cited by 60 publications

References 110 publications

Transcriptomic analysis of the impacts of ethinylestradiol (EE2) and its consequences for proliferative kidney disease outcome in rainbow trout (Oncorhynchus mykiss)

Transcriptomic analysis of the impacts of ethinylestradiol (EE2) and its consequences for proliferative kidney disease outcome in rainbow trout (Oncorhynchus mykiss)

Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans

Cell-to-cell expression dispersion of B-cell surface proteins displays genetic variation among humans

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