Expression and Function of RIG-I in Oral Keratinocytes and Fibroblasts

Ohta, Kouji; Fukui, Akiko; Shigeishi, Hideo; Ishida, Yasushi; Nishi, Hiromi; Tobiume, Kei; Takechi, Masaaki; Kamata, Nobuyuki

doi:10.1159/000366359

Cited by 17 publications

(28 citation statements)

References 30 publications

(39 reference statements)

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“…Keratinocytes of the RT7 immortalized human oral keratinocyte cell line were established by transfection of human telomerase reverse transcriptase and E7, as previously described (60), and were then cultured in keratinocyte growth medium (KGM) supplemented with human epidermal growth factor, insulin, hydrocortisone, calcium, bovine pituitary extract, gentamicin sulfate, and amphotericin B (Lonza, Walkersville, MD). We also prepared primary cultures of human oral keratinocytes obtained from healthy gingival tissues excised during extraction of impacted teeth from 3 different patients, as previously reported (61). Informed consent for acquisition of those tissues was obtained according to a protocol approved by the Ethical Committee of Hiroshima University.…”

Section: Methodsmentioning

confidence: 99%

Candida albicans β-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway

et al. 2018

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Oral keratinocytes provide the first line of host defense against oral candidiasis. We speculated that interactions of fungal cell wall components with oral keratinocytes regulate the stress response against infection and examined the expression of genes induced by heat-killed in oral immortalized keratinocytes using a cDNA microarray technique. Of 24,000 genes revealed by that analysis, we focused on HO-1, a stress-inducible gene, as its expression was increased by both heat-killed and live In histological findings, HO-1 expression in the superficial layers of the oral epithelium following infection was elevated compared to that in healthy epithelium. We then investigated fungal cell wall components involved in induction of HO-1 expression and found that β-glucan-containing particles (β-GPs) increased its expression. Furthermore, β-glucan was observed on the surface of both heat-killed and cells that had invaded the oral epithelium. Fungal β-GPs also promoted induction of intracellular reactive oxygen species (ROS), NADPH oxidase activation, and p38 mitogen-activated protein kinase (MAPK) phosphorylation, while those specific inhibitors inhibited the HO-1 expression induced by fungal β-GPs. Moreover, fungal β-GPs induced Nrf2 translocation into nuclei via p38 MAPK signaling, while the HO-1 expression induced by fungal β-GPs was inhibited by Nrf2-specific small interfering RNA (siRNA). Finally, knockdown of cells by HO-1- and Nrf2-specific siRNAs resulted in increased β-GP-mediated ROS production compared to that in the control cells. Our results show that the HO-1 induced by fungal β-GPs via ROS/p38 MAPK/Nrf2 from oral keratinocytes may have important roles in host defense against the stress caused by infection in the oral epithelium.

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Section: Methodsmentioning

confidence: 99%

Candida albicans β-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway

et al. 2018

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“…RIG‐I‐like receptors (RLR) are cytoplasmic RNA helicases, which are expressed at low levels in resting cells, but their expression is strongly induced during viral infection by secretion of type I interferons as well as proinflammatory cytokines including IFN‐γ, IL‐1β and TNF . It has been demonstrated that RIG‐I is constitutively expressed in cells of the connective tissue including fibroblasts, epithelial and endothelial cells . Moreover, it has been shown that RIG‐I‐deficient MEF failed to generate IFN‐β upon viral infection, while virus‐infected dendritic cells from RIG‐I −/− mice secrete large amounts of type I IFN , indicating that RIG‐I plays an important role in non‐professional immune cells.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies reveal that the RLR play an important role in the antiviral response of non‐professional immune cells, including fibroblasts, keratinocytes, endothelial cells and epithelial cells . While resting cells show relatively low levels of RLR, their expression is strongly induced after viral infection and exposure to type I interferons (IFN) .…”

Section: Introductionmentioning

confidence: 99%

Oncostatin M induces RIG‐I and MDA5 expression and enhances the double‐stranded RNA response in fibroblasts

Hergovits

Mais

Haan

et al. 2017

J Cellular Molecular Medi

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Interleukin (IL)‐6‐type cytokines have no direct antiviral activity; nevertheless, they display immune‐modulatory functions. Oncostatin M (OSM), a member of the IL‐6 family, has recently been shown to induce a distinct number of classical interferon stimulated genes (ISG). Most of them are involved in antigen processing and presentation. However, induction of retinoic acid‐inducible gene (RIG)‐I‐like receptors (RLR) has not been investigated. Here we report that OSM has the capability to induce the expression of the DExD/H‐Box RNA helicases RIG‐I and melanoma differentiation antigen 5 (MDA5) as well as of the transcription factors interferon regulatory factor (IRF)1, IRF7 and IRF9 in primary fibroblasts. Induction of the helicases depends on tyrosine as well as serine phosphorylation of STAT1. Moreover, we could show that the OSM‐induced STAT1 phosphorylation is predominantly counter‐regulated by a strong STAT3‐dependent SOCS3 induction, as Stat3 as well as Socs3 knock‐down results in an enhanced and prolonged helicase and IRF expression. Other factors involved in regulation of STAT1 or IRF1 activity, like protein tyrosine phosphatase, non‐receptor type 2 (PTPN2), promyelocytic leukaemia protein (PML) or small ubiquitin‐related modifier 1 (SUMO1), play a minor role in OSM‐mediated induction of RLR. Remarkably, OSM and interferon‐γ (IFN‐γ) synergize to mediate transcription of RLR and pre‐treatment of fibroblasts with OSM fosters the type I interferon production in response to a subsequent encounter with double‐stranded RNA. Together, these findings suggest that the OSM‐induced JAK/STAT1 signalling is implicated in virus protection of non‐professional immune cells and may cooperate with interferons to enhance RLR expression in these cells.

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“…Expression levels of receptor-related genes (TLRs [14], NLRs [15,16], and RLRs [17]), which recognize bacterial components, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH as…”

Section: Quantitative Rt-pcr (Rt-qpcr)mentioning

confidence: 99%

Ultrafine Bubble Water Usefulness in Fecal Microbiota Transplantation: Recognition of Transplanted Microbiota in Intestinal Epithelial Cells

Shimizu¹,

Dan²,

Tanaka³

et al. 2020

BCHD

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Background: Indications for various diseases such as diabetes mellitus and metabolic syndrome, etc. of Fecal Microbiota Transplantation (FMT) have been investigated. For the establishment of the FMT therapy, the examination of the solvent has not been carried out, though the use of the physiological saline is fixed, at present. We have produced the Mr. Shimizu made ultrafine bubble water (UFB) that produces a larger number of bubbles than existing UFB.Objective: To verify the usefulness of UFB, we prepared a conventional Saline and UFB-prepared microbial preparation (Bio3); (Bio3/Saline) and (Bio3/UFB). The bacterial preparations, Bio3, contain glycated bacteria, lactic acid bacteria, and butyric acid bacteria. FMT was carried out to the diabetic model mouse using these microbial preparations, and whether the disease state was improved was examined. Cultured intestinal epithelial cells (CaCO-2) were also used to test for differentiation by UFB and Saline from variations in several receptors that recognize microbiota-mRNA. In addition, glucose uptake into cells was measured.Methods: FMT with (Bio3/UFB) and (Bio3/Saline) was performed in streptozotocin-induced diabetic mice (STZ-mice) in duplicate at 0, 7 days. Blood glucose levels (7, 14 days) and blood insulin levels (14 days) were measured. Cultured intestinal epithelial cells were also used to test for differentiation by UFB and Saline from change in several receptors that recognize microbiota-mRNA (Toll-like receptors, NOD-like receptors, and RIG-I-like receptors). In addition, glucose uptake into cells was measured using fluorescently labeled glucose analog (NBDG).Results: UFB could reduce the blood glucose level of STZ-induced diabetic model mice. However, no such effects were observed in Saline. Stimulation of serially diluted Bio3 with UFB-suspension were showed significant alteration in TLR4 and IL-1B-uPNA. The amount of glucose uptake in the (Bio3/UFB) group was significantly different at 30 min, inhibited or delayed. Conclusion: It is concluded that UFB-mediated cross-talk between intestinal bacteria and intestinal epithelial cells and inhibition or delay of intestinal epithelial glucose uptake may have been associated with the reduction of blood glucose levels in diabetic model mice. The superiority of UFB as a suspension used for the transfer of bacteria has been suggested.

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Expression and Function of RIG-I in Oral Keratinocytes and Fibroblasts

Cited by 17 publications

References 30 publications

Candida albicans β-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway

Candida albicans β-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway

Oncostatin M induces RIG‐I and MDA5 expression and enhances the double‐stranded RNA response in fibroblasts

Ultrafine Bubble Water Usefulness in Fecal Microbiota Transplantation: Recognition of Transplanted Microbiota in Intestinal Epithelial Cells

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