Expression and function of dopamine in odontoblasts

Fujino, Shoko; Tomokiyo, Atsushi; Itoyama, Tomohiro; Hasegawa, Daigaku; Sugii, Hideki; Yoshida, Shinichiro; Washio, Ayako; Nozu, Aoi; Ono, Taiga; Wada, Naohisa; Kitamura, Chiaki; Maeda, Hidefumi

doi:10.1002/jcp.29314

Cited by 7 publications

(8 citation statements)

References 35 publications

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“…The released ATP, glutamate, and ADP (which is subsequently hydrolyzed from ATP) establish a paracrine signaling network between odontoblasts through the activation of ionotropic ATP (P2X) receptors, metabotropic glutamate receptors, and metabotropic ADP (P2Y) receptors, respectively; an intercellular odontoblast paracrine/autocrine communication is thereby established. The expression of both DA and TH as well as the DA receptor in odontoblasts [16], taken together with the present results, suggests that DA-induced cAMP signaling following G s protein-coupled DA receptor activation may establish intercellular odontoblast paracrine and/or autocrine communication to promote odontoblastic differentiation and dentinogenesis. However, further studies are required to confirm this hypothesis.…”

Section: Discussionsupporting

confidence: 76%

“…In addition, recent studies have shown that rat odontoblasts are immunoreactive to G s protein-coupled β 2 and calcitonin gene-related peptide (CGRP) receptor antibodies [ 12 , 13 ], and mouse odontoblasts express the parathyroid hormone receptor as observed in in situ hybridization assays [ 14 ]. The immunofluorescent expression of prostaglandin (PG) I 2 (IP) receptors [ 15 ] and the mRNA expression of dopamine (DA) D 1 (D 1 ) receptors in rat odontoblasts have also been reported [ 16 ]. Human dental pulp cells express mRNAs of all four adenosine receptor subtypes (A 1 , A 2A , A 2B , and A 3 receptors) in RT-PCR analysis.…”

Section: Introductionmentioning

confidence: 99%

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Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line

Kitayama,

Kimura,

Ouchi

et al. 2023

Biomolecules

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Odontoblasts are involved in sensory generation as sensory receptor cells and in dentin formation. We previously reported that an increase in intracellular cAMP levels by cannabinoid 1 receptor activation induces Ca2+ influx via transient receptor potential vanilloid subfamily member 1 channels in odontoblasts, indicating that intracellular cAMP/Ca2+ signal coupling is involved in dentinal pain generation and reactionary dentin formation. Here, intracellular cAMP dynamics in cultured human odontoblasts were investigated to understand the detailed expression patterns of the intracellular cAMP signaling pathway activated by the Gs protein-coupled receptor and to clarify its role in cellular functions. The presence of plasma membrane Gαs as well as prostaglandin I2 (IP), 5-hydroxytryptamine 5-HT4 (5-HT4), dopamine D1 (D1), adenosine A2A (A2A), and vasoactive intestinal polypeptide (VIP) receptor immunoreactivity was observed in human odontoblasts. In the presence of extracellular Ca2+, the application of agonists for the IP (beraprost), 5-HT4 (BIMU8), D1 (SKF83959), A2A (PSB0777), and VIP (VIP) receptors increased intracellular cAMP levels. This increase in cAMP levels was inhibited by the application of the adenylyl cyclase (AC) inhibitor SQ22536 and each receptor antagonist, dose-dependently. These results suggested that odontoblasts express Gs protein-coupled IP, 5-HT4, D1, A2A, and VIP receptors. In addition, activation of these receptors increased intracellular cAMP levels by activating AC in odontoblasts.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line

Kitayama,

Kimura,

Ouchi

et al. 2023

Biomolecules

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show abstract

“…Recent reports have shown that DA promotes osteoblastic differentiation of bone marrow stem cells and MC3T3-E1 cells 18,19 . Furthermore, in our previous report, we revealed that the involvement of DA in the maturation of odontoblasts at the stage of tooth development 20 . However, there is no report about the effect of DA on odontoblast-like differentiation of DPSCs in the process of reparative dentin formation.…”

mentioning

confidence: 72%

“…Moreover, these factors are expressed in non-neural cells such as those in the heart and pancreas 24,25 . Even in osteoblasts and pre-odontoblasts, these factors are expressed and promote their differentiation 20,26 . Taken together, we demonstrated that TH and DA were involved in odontoblastic differentiation of DPSCs during reparative dentin formation.…”

Section: Discussionmentioning

confidence: 99%

Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

Fujino

Tomokiyo

Sugiura

et al. 2023

Sci Rep

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Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

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“…A reason for these seemingly conflicting results could be that catecholamine might also be produced by non‐neural cells in the dental pulp. For example, odontoblasts have been verified to express tyrosine hydroxylase, the rate‐limiting enzyme for catecholamine synthesis (Fujino et al., 2020). Importantly, sympathetic nerves tend to mediate the immune response through the release of catecholamine and neuropeptide Y in dental pulp (Haug & Heyeraas, 2005).…”

Section: Discussionmentioning

confidence: 99%

Sensory nerves, but not sympathetic nerves, promote reparative dentine formation after dentine injury via CGRP‐mediated angiogenesis: An in vivo study

Zhan,

Huang,

Chen

et al. 2023

Int Endodontic J

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AimDental pulp is richly innervated by nerve fibres, which are mainly involved in the sensation of pain. Aside from pain sensation, little is known regarding the role of dental innervation in reparative dentine formation. We herein generated a mouse model of experimental dentine injury to examine nerve sprouting within the odontoblast and subodontoblastic layers and investigated the potential effects of this innervation in reparative dentinogenesis.MethodologyMouse tooth cavity model (bur preparation + etching) was established, and then nerve sprouting, angiogenesis and reparative dentinogenesis were determined by histological and immunofluorescent staining at 1, 3, 7, 14 and 28 days postoperatively. We also established the mouse‐denervated molar models to determine the role of sensory and sympathetic nerves in reparative dentinogenesis, respectively. Finally, we applied calcitonin gene‐related peptide (CGRP) receptor antagonist to analyse the changes in angiogenesis and reparative dentinogenesis.ResultsSequential histological results from dentine‐exposed teeth revealed a significant increase in innervation directly beneath the injured area on the first day after dentine exposure, followed by vascularisation and reparative dentine production at 3 and 7 days, respectively. Intriguingly, abundant type H vessels (CD31+Endomucin+) were present in the innervated area, and their formation precedes the onset of reparative dentine formation. Additionally, we found that sensory denervation led to blunted angiogenesis and impaired dentinogenesis, while sympathetic denervation did not affect dentinogenesis. Moreover, a marked increase in the density of CGRP+ nerve fibres was seen on day 3, which was reduced but remained elevated over the baseline level on day 14, whereas the density of substance P‐positive nerve fibres did not change significantly. CGRP receptor antagonist‐treated mice showed similar results as those with sensory denervation, including impairments in type H angiogenesis, which confirms the importance of CGRP in the formation of type H vessels.ConclusionsDental pulp sensory nerves act as an essential upstream mediator to promote angiogenesis, including the formation of type H vessels, and reparative dentinogenesis. CGRP signalling governs the nerve‐vessel‐reparative dentine network, which is mostly produced by newly dense sensory nerve fibres within the dental pulp.

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Expression and function of dopamine in odontoblasts

Cited by 7 publications

References 35 publications

Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line

Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line

Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

Sensory nerves, but not sympathetic nerves, promote reparative dentine formation after dentine injury via CGRP‐mediated angiogenesis: An in vivo study

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