Expression and function of 5-hydroxytryptamine 4 receptors in smooth muscle preparations from the duodenum, ileum, and pelvic flexure of horses without gastrointestinal tract disease

Prause, Andrea S.; Guionaud, Christophe; Stoffel, Michael; Portier, Christopher J.; Mevissen, Meike

doi:10.2460/ajvr.71.12.1432

Cited by 11 publications

(9 citation statements)

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“…The present study provides solid evidence of 5‐HT 4 ‐IR in the MP and SMP neurons of horse intestine. Despite some functional investigations carried out on horse small and large intestine indicated the presence of 5‐HT 4 in the ENS of this animal model, the only immunohistochemical study carried out on horse ENS failed to detect 5‐HT 4 in enteric neurons, but confirmed the presence of this serotonergic receptor on muscular layers . Furthermore, a recent study by Delesalle et al .…”

Section: Discussionmentioning

confidence: 80%

“…Despite some functional investigations carried out on horse small and large intestine indicated the presence of 5-HT 4 in the ENS of this animal model, [23][24][25][26][27][28][29][30][31][32][33] the only immunohistochemical study carried out on horse ENS failed to detect 5-HT 4 in enteric neurons, but confirmed the presence of this serotonergic receptor on muscular layers. 31 Furthermore, a recent study by Delesalle et al 39 receptor were already observed in the rat spinal ganglia. 22 As 5-HT 4 -IR was co-expressed by extrinsic SP-IR sensory fibers of aganglionotic LWFS foals, it is plausible that this receptor might play a role in nociception.…”

Section: Discussionmentioning

confidence: 91%

“…Indeed, 5-HT 4 seems to play a fundamental role in GI physiology and seems to be a promising pharmacological target for equine GI disorders. [23][24][25][26][27][28][29][30][31][32][33] In addition, we investigated 5-HT 4 expression in visceral extrinsic sensory nerves by analyzing spinal ganglion neurons from healthy horses and intestinal tissues from foals with lethal white foal syndrome (LWFS), i.e., ileocolonic aganglionosis, reminiscent of Hirschsprung's disease in humans. 34…”

Section: Introductionmentioning

confidence: 99%

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Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Giancola

Rambaldi

Bianco

et al. 2017

Neurogastroenterology Motil

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Giancola

Rambaldi

Bianco

et al. 2017

Neurogastroenterology Motil

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“…5-HT 4 receptors, on the other hand, are G protein-coupled receptors that stimulate cAMP production (30). 5-HT 4 receptors are localized in the enteric neurons (35), smooth muscles (32), and epithelial cells including the EC cells (15). Effects of 5-HT mediated secretion in Ussing chamber involve activation of both neuronal and nonneuronal 5-HT 3 and 5-HT 4 receptors (6,26,31).…”

mentioning

confidence: 99%

Human-derived gut microbiota modulates colonic secretion in mice by regulating 5-HT₃ receptor expression via acetate production

Bhattarai

Schmidt

Linden

et al. 2017

American Journal of Physiology-Gastrointestinal and Liver Physi

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Serotonin [5-hydroxytryptamine (5-HT)], an important neurotransmitter and a paracrine messenger in the gastrointestinal tract, regulates intestinal secretion by its action primarily on 5-HT and 5-HT receptors. Recent studies highlight the role of gut microbiota in 5-HT biosynthesis. In this study, we determine whether human-derived gut microbiota affects host secretory response to 5-HT and 5-HT receptor expression. We used proximal colonic mucosa-submucosa preparation from age-matched Swiss Webster germ-free (GF) and humanized (HM; ex-GF colonized with human gut microbiota) mice. 5-HT evoked a significantly greater increase in short-circuit current (Δ) in GF compared with HM mice. Additionally, 5-HT receptor mRNA and protein expression was significantly higher in GF compared with HM mice. Ondansetron, a 5-HT receptor antagonist, inhibited 5-HT-evoked Δ in GF mice but not in HM mice. Furthermore, a 5-HT receptor-selective agonist, 2-methyl-5-hydroxytryptamine hydrochloride, evoked a significantly higher Δ in GF compared with HM mice. Immunohistochemistry in 5-HT-green fluorescent protein mice localized 5-HT receptor expression to enterochromaffin cells in addition to nerve fibers. The significant difference in 5-HT-evoked Δ between GF and HM mice persisted in the presence of tetrodotoxin (TTX) but was lost after ondansetron application in the presence of TTX. Application of acetate (10 mM) significantly lowered 5-HT receptor mRNA in GF mouse colonoids. We conclude that host secretory response to 5-HT may be modulated by gut microbiota regulation of 5-HT receptor expression via acetate production. Epithelial 5-HT receptor may function as a mediator of gut microbiota-driven change in intestinal secretion. We found that gut microbiota alters serotonin (5-HT)-evoked intestinal secretion in a 5-HT receptor-dependent mechanism and gut microbiota metabolite acetate alters 5-HT receptor expression in colonoids.View this article's corresponding video summary at https://www.youtube.com/watch?v=aOMYJMuLTcw&feature=youtu.be.

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“…5-HT4 receptors, linked to adenylyl cyclase, act via generation of cAMP. None of the 4 in vitro studies on 5-HT in horses provided evidence for neuronal 5-HT4 receptors, but none used the protocol as described in human studies [1][2][3][4].…”

Section: Ethical Animal Researchmentioning

confidence: 99%

Identification of Disease Specific Metabolic Fingerprints in Early Osteoarthritis

Peffers

Riggs

Phelan

et al. 2015

Equine Veterinary Journal

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Clinical Research Abstracts British Equine Veterinary Association Congress 2015 Foreword and AcknowledgementsThis supplement is devoted to the publication of abstracts from the 2015 Congress of The British Equine Veterinary Association. The quality of research at the Congress was, as in previous years, high, and the selection process was difficult. Despite the inclusion of a larger number of research communications than in previous years only two-thirds of those submitted could be accepted. This year's Congress committee utilised the selection process initiated last year by Professor Celia Marr in partnership with EVJ. Abstracts were reviewed and objectively graded by two peer reviewers and a member of the Congress Committee before being subjected to a final selection process overseen by the Congress Chair. Thereafter, abstracts had to satisfy the requirements of EVJ for publication in this supplement. Those authors who have had their work accepted should be proud of their achievement. Those who were unsuccessful are doubtless disappointed; however, they have the reassurance that the standard was high and the process was fair. deserve thanks and recognition for a year of hard work. Particular thanks also to all of the anonymous peer reviewers who receive no recognition but gave their time not only to grade the abstracts submitted but also to provide constructive feedback to their authors.The selection process adopted for BEVA Congress sets a standard for other meetings to follow. Given the increasing competition for acceptance of research communications at major international meetings, and the importance of achieving acceptance for those seeking to fulfil the requirements of specialist colleges, such a rigourous selection process seems only right.Reasons for performing study: There are limited data surrounding the efficacy of oviductal flushing in restoring fertility in mares with repeated returns to oestrus. Objectives:To determine the conception and foaling rates in mares treated by laparoscopic topical application of prostaglandin E2 (PGE2) to the oviduct(s) in mares: i) in the breeding season (January-May), pre-covering; ii) in the breeding season, post covering; and iii) out of the breeding season (June-December).Study design: Follow-up study.Methods: Analysis of electronic patient records (EPRs) identified 29 mares that underwent oviductal flushing via laparoscopic surgery between 1 January 2008 and 12 October 2014. One mare underwent the procedure twice; once in the breeding season (pre-covering) and once out of the breeding season. Follow-up data were collected via EPRs and from Weatherbys (www.bloodstockreports.co.uk). Results:The procedure was used in 27 Thoroughbreds, one Warmblood and one Hanoverian. Mean age at the time of the procedure was 13.1 years (range 6-22 years). Mean duration barren prior to the procedure was 1.9 years (range 49 days-5.6 years). The mean time post flushing to the first positive ultrasound scan was 31 days, with average time post flushing to foaling of 367.6 days. Of the 29 h...

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Expression and function of 5-hydroxytryptamine 4 receptors in smooth muscle preparations from the duodenum, ileum, and pelvic flexure of horses without gastrointestinal tract disease

Abstract: In horses, 5-HT4 receptors on smooth muscle cells appear to be involved in the contractile response of the intestinal tract to 5-hydroxytryptamine. Results suggest that tegaserod may be useful for treatment of reduced gastrointestinal tract motility in horses.

Cited by 11 publications

References 50 publications

Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Human-derived gut microbiota modulates colonic secretion in mice by regulating 5-HT₃ receptor expression via acetate production

Identification of Disease Specific Metabolic Fingerprints in Early Osteoarthritis

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Expression and function of 5-hydroxytryptamine 4 receptors in smooth muscle preparations from the duodenum, ileum, and pelvic flexure of horses without gastrointestinal tract disease

Abstract: In horses, 5-HT4 receptors on smooth muscle cells appear to be involved in the contractile response of the intestinal tract to 5-hydroxytryptamine. Results suggest that tegaserod may be useful for treatment of reduced gastrointestinal tract motility in horses.

Cited by 11 publications

References 50 publications

Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Localization of the 5‐hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers

Human-derived gut microbiota modulates colonic secretion in mice by regulating 5-HT3 receptor expression via acetate production

Identification of Disease Specific Metabolic Fingerprints in Early Osteoarthritis

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Human-derived gut microbiota modulates colonic secretion in mice by regulating 5-HT₃ receptor expression via acetate production