Expression and evolution of the phosphoinositide-specific phospholipase C gene family in Arabidopsis thaliana

Tasma, I Made; Brendel, Volker; Whitham, Steven A.; Bhattacharyya, Madan K.

doi:10.1016/j.plaphy.2008.04.015

Cited by 106 publications

(128 citation statements)

References 39 publications

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“…AtPLC2 is expressed constitutively, but expressions of the remaining eight PI-PLC genes have been shown to be regulated by salt, cold and dehydration stress, abscisic acid, and other perturbations (Tasma et al, 2008). Interestingly, the expression of AtPLC4 and AtPLC5 genes is regulated by nitrate in Arabidopsis roots (Wang et al, 2003(Wang et al, , 2004Vidal et al, 2013aVidal et al, , 2013bAlvarez et al, 2014;Canales et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis

Riveras

Álvarez²,

Vidal³

et al. 2015

Plant Physiol.

198

141

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Understanding how plants sense and respond to changes in nitrogen availability is the first step toward developing strategies for biotechnological applications, such as improvement of nitrogen use efficiency. However, components involved in nitrogen signaling pathways remain poorly characterized. Calcium is a second messenger in signal transduction pathways in plants, and it has been indirectly implicated in nitrate responses. Using aequorin reporter plants, we show that nitrate treatments transiently increase cytoplasmic Ca 2+ concentration. We found that nitrate also induces cytoplasmic concentration of inositol 1,4,5-trisphosphate. Increases in inositol 1,4,5-trisphosphate and cytoplasmic Ca 2+ levels in response to nitrate treatments were blocked by U73122, a pharmacological inhibitor of phospholipase C, but not by the nonfunctional phospholipase C inhibitor analog U73343. In addition, increase in cytoplasmic Ca 2+ levels in response to nitrate treatments was abolished in mutants of the nitrate transceptor NITRATE TRANSPORTER1.1/Arabidopsis (Arabidopsis thaliana) NITRATE TRANSPORTER1 PEPTIDE TRANSPORTER FAMILY6.3. Gene expression of nitrate-responsive genes was severely affected by pretreatments with Ca 2+ channel blockers or phospholipase C inhibitors. These results indicate that Ca 2+ acts as a second messenger in the nitrate signaling pathway of Arabidopsis. Our results suggest a model where NRT1.1/AtNPF6.3 and a phospholipase C activity mediate the increase of Ca 2+ in response to nitrate required for changes in expression of prototypical nitrate-responsive genes.

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Section: Discussionmentioning

confidence: 99%

The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis

Riveras

Álvarez²,

Vidal³

et al. 2015

Plant Physiol.

198

141

View full text Add to dashboard Cite

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“…These data suggest that GmPI-PLC is activated by other mechanisms, such as by ABA. In A. thaliana, members of the PLC gene family are activated by ABA (Tasma et al, 2008). Enhanced expression of ZmPLC1 also improves drought tolerance in maize: under drought stress conditions, transgenic plants have higher relative water content, better osmotic adjustment, increased photosynthetic rates, a lower percentage of ion leakage, less lipid membrane peroxidation and higher grain yield when compared to wild-type maize (Wang et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Polizel¹,

Medri²,

Nakashima³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydrationresponsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T 2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T 2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance.

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“…S2D legend in the supplemental material). These discrepancies probably reflect the complex phylogenetic relationships of the eukaryotic PI-PLC family, as has been previously suggested by several researchers in the field (4,24,43). Nevertheless, in all aspects studied, TtPLC3 is most likely an inactive PI-PLC and a true PRIP homolog, since it lacks the two critical His residues necessary for catalysis (Fig.…”

Section: Resultsmentioning

confidence: 78%

“…Importantly, all unicellular organisms studied, including fungi (Saccharomyces cerevisiae), slime molds (Dictyostelium discoideum), kinetoplastids (Trypanosoma cruzi), and apicomplexa (Toxoplasma gondii), have a single PI-PLC gene (10,12,13,34,36), while Arabidopsis thaliana has at least nine PI-PLC genes (4,43). In animals, six catalytically functional PI-PLC isoforms have been described, namely, ␦, ␤, ␥, ε, , and (24).…”

Section: Regulatory Axis In Ciliatesmentioning

confidence: 99%

Biochemical and Genetic Evidence for the Presence of Multiple Phosphatidylinositol- and Phosphatidylinositol 4,5-Bisphosphate-Specific Phospholipases C in Tetrahymena

et al. 2011

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, hydrolyze PtdIns and glycosyl-PtdIns, and they are considered important virulence factors. All unicellular eukaryotes studied so far contain a single PI-PLC-like gene. In this report, we show that ciliates are an exception, since we provide evidence that Tetrahymena species contain two sets of functional genes coding for both bacterial and eukaryotic PLCs. Biochemical characterization revealed two PLC activities that differ in their phosphoinositide substrate utilization, subcellular localization, secretion to extracellular space, and sensitivity to Ca 2؉ . One of these activities was identified as a typical membrane-associated PI-PLC activated by low-micromolar Ca 2؉ , modestly activated by GTP␥S in vitro, and inhibited by the compound U73122 [1-(6-{[17␤-3-methoxyestra-1,3,5(10)-trien-17-yl]amino}hexyl)-1H-pyrrole-2,5-dione]. Importantly, inhibition of PI-PLC in vivo resulted in rapid upregulation of PtdIns(4,5)P 2 levels, suggesting its functional importance in regulating phosphoinositide turnover in Tetrahymena. By in silico and molecular analysis, we identified two PLC genes that exhibit significant similarity to bacterial but not trypanosomal PLC genes and three eukaryotic PI-PLC genes, one of which is a novel inactive PLC similar to proteins identified only in metazoa. Comparative studies of expression patterns and PI-PLC activities in three T. thermophila strains showed a correlation between expression levels and activity, suggesting that the three eukaryotic PI-PLC genes are functionally nonredundant. Our findings imply the presence of a conserved and elaborate PI-PLC-Ins(1,4,5)P 3 -Ca 2؉ regulatory axis in ciliates.Phosphoinositides (PIs), i.e., phosphatidylinositol (PtdIns) and its phosphorylated derivatives, are ubiquitous constituents of eukaryotic membranes, with many and diverse functions in receptor signaling, membrane trafficking, and cytoskeleton organization. Among PI-metabolizing enzymes, phospholipases C (PLCs) that hydrolyze PtdIns and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P 2 ] are of particular importance because they generate second messengers such as inositol 1, 4,5-trisphosphate [Ins(1,4,5)P 3 ] and participate in the replenishment of the inositol pool available for phosphoinositide synthesis. These phospholipases fall into distinct families: (i) the well-established family of eukaryotic phosphoinositide-specific PLCs (PI-PLCs) (EC 3

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Expression and evolution of the phosphoinositide-specific phospholipase C gene family in Arabidopsis thaliana

Cited by 106 publications

References 39 publications

The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis

The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Biochemical and Genetic Evidence for the Presence of Multiple Phosphatidylinositol- and Phosphatidylinositol 4,5-Bisphosphate-Specific Phospholipases C in Tetrahymena

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