Expression and effect of fibroblast growth factor 9 in bovine theca cells

Schreiber, Nicole B.; Totty, M.; Spicer, L. J.

doi:10.1530/joe-12-0293

Cited by 24 publications

(37 citation statements)

References 61 publications

(79 reference statements)

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“…The FGF family members have also been identified to play important roles in the reproduction of mammals including the human, caprine, ovine, bovine and murine species (for review see Chaves et al, 2012). To date, FGF1, FGF2, FGF7–9, FGF10, FGF17, and FGF18 have been identified in the ovary of humans (Oron et al, 2012), rodents (Drummond et al, 2007), or domestic animals (Berisha et al, 2004; Machado et al, 2009; Portela et al, 2010; Schreiber et al, 2012). Functions of FGF members in the ovary include regulation of granulosa cell ( GC ) steroidogenesis of cattle (Vernon and Spicer, 1994; Schreiber and Spicer, 2012) and pigs (Evans et al, 2014), regulation of GC apoptosis and survival in rats (Tilly et al, 1992; Peluso et al, 2001) and cattle (Portela et al, 2010; Jiang and Price, 2012), control of bovine GC proliferation and differentiation (Berisha et al, 2004; Schreiber and Spicer, 2012), oocyte maturation in rats (LaPolt et al, 1990) and cattle (Cho et al, 2008), and luteal development in cattle (Gabler et al, 2004; Woad et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, abundance of FGF9 mRNA was found to be greater in GC than in theca cells ( TC ) and greater in both GC and TC in small follicles (i.e., 1–5 mm) in comparison to large (i.e., ≥8 mm) follicles (Schreiber et al, 2012). Hormones known to be involved in follicular development (e.g., IGF-1) also regulate FGF9 mRNA in both GC (Schreiber and Spicer, 2012) and TC (Schreiber et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Changes in fibroblast growth factor 9 mRNA in granulosa and theca cells during ovarian follicular growth in dairy cattle

Schütz

Schreiber

Gilliam

et al. 2016

Journal of Dairy Science

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Fibroblast growth factor 9 (FGF9) has been suggested to act as an antidifferentiation factor in cattle by reducing steroidogenesis and increasing cell proliferation in granulosa (GC) and theca (TC) cells. The objective of this study was to characterize FGF9 mRNA abundance in GC and TC during development of dominant follicles in dairy cattle. Estrous cycles of nonlactating dairy cattle were synchronized, and ovaries were collected on either d 3 to 4 (n = 8) or 5 to 6 (n = 8) postovulation for GC and TC RNA extraction from small (1–5 mm), medium (5.1–8 mm), and large (8.1–18 mm) follicles for PCR analysis. The FGF9 mRNA abundance was greater in GC than in TC. In GC, FGF9 mRNA abundance was greater in small, medium, and large estrogen-inactive [i.e., concentrations of estradiol (E2) < progesterone (P4)] follicles than in large E2-active (i.e., concentrations of E2 > P4) follicles at both early (d 3–4) and late (d 5–6) growing phases of first dominant follicle. Abundance of FGF9 mRNA increased in medium-sized follicles from early to late growing phase of the dominant follicle. In TC, FGF9 mRNA abundance was greater in large E2-inactive follicles than in large E2-active follicles on d 3 to 4 postovulation; no significant differences in TC FGF9 mRNA existed among follicle types on d 5 to 6 postovulation. Correlations among levels of follicular fluid hormones and FGF9 mRNA levels revealed significant negative correlations between GC FGF9 mRNA abundance and follicular fluid E2 (r = −0.68), free IGF-1 (r = −0.63), and E2-to-P4 ratio (r = −0.58). In summary, abundance of FGF9 mRNA in GC and TC increases in medium-sized follicles during development of dominant follicles and is less in dominant E2-active than subordinate E2-inactive follicles, suggesting that FGF9 signaling could contribute to normal follicle development and steroidogenesis in dairy cattle.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in fibroblast growth factor 9 mRNA in granulosa and theca cells during ovarian follicular growth in dairy cattle

Schütz

Schreiber

Gilliam

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Because of that, in experiments 2 and 3, theca cells were treated with LH (100ng mL -1 ) and insulin (100ng mL -1 ). This experiment was performed to verify a possible stimulatory effect on mRNA expression of steroidogenic enzymes, as well as, on androstenedione and testosterone production according to the methodology proposed by COMIM et al (2013) andSCHREIBER et al (2012).…”

Section: Resultsmentioning

confidence: 99%

“…The collected theca tissues were digested in a collagenase solution (1mg mL -1 ) for cell separation and the obtained cells were seeded in 60mm culture plates. Cells were cultured for 48 hours in a basic medium: (DMEM/F12 supplemented with 10% fetal bovine serum (FBS), 1µg mL -1 transferrin, 1ng/mL selenium, 100UI mL -1 penicillin, 100µg mL -1 streptomycin and 2.5µg mL -1 amphotericin), at 38.5 o C and 5% of CO 2 as described previously (COMIM et al, 2013;SCHREIBER et al, 2012). After this period, the cells used in experiment 1 were trypsinized and seeded (2x10 5 cells well -1 ) in 4-well culture plates (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%