Expression and distribution of leptin and its receptors in the digestive tract of DIO (diet-induced obese) zebrafish

Mania, Manuela; Maruccio, Lucianna; Russo, Finizia; Abbate, Francesco; Castaldo, L.; D’Angelo, Livia; Girolamo, Paolo de; Guerrera, Maria Cristina; Lucini, Carla; Madrigrano, M.; Levanti, Maria; Germanà, Antonino

doi:10.1016/j.aanat.2017.03.005

Cited by 22 publications

(17 citation statements)

References 69 publications

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“…Indeed, the mRNA expression decreases considerably in response to fasting. In a recent paper published by our research group, we demonstrated the presence of leptin protein and its receptor in the digestive tract in diet‐induced obesity zebrafish by Western blotting and immunohistochemistry and an increase in leptin immunoreactive cells in obese model compared to normally fed zebrafish (Mania et al., ). Moreover, Michel, Page‐McCaw, Chen, and Cone () demonstrated that adult zebrafish lacking a functional leptin receptor do not exhibit hyperphagia or increased adiposity, but regulate glucose homoeostasis, as in mammals, suggesting that glucose homoeostasis appears to be conserved across vertebrates.…”

Section: Discussionmentioning

confidence: 90%

Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting

García‐Suárez

Cabo

Abbate

et al. 2018

Anat Histol Embryol

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Leptin is an anorectic hormone secreted mainly by peripheral adipocytes but also by other central and peripheral tissues. It acts by means of a receptor called OB-R, influencing not only appetite and body mass but being also involved in many fields like endocrinology, metabolism and reproduction. Immunohistochemistry and qRT-PCR techniques were, respectively, used to demonstrate the presence of leptin and its receptor in the gut of adult zebrafish and to evaluate the leptin gene expression response to feeding and fasting. Immunoreactivity for the antibodies utilized was demonstrated in feeding but not in fasting fish, and the gene expression analysis corroborates the data obtained by immunohistochemistry. Therefore, all the obtained results support the hypothesis of the role of this hormone in food regulation in zebrafish.

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Section: Discussionmentioning

confidence: 90%

Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting

García‐Suárez

Cabo

Abbate

et al. 2018

Anat Histol Embryol

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“…The D.I.O. feeding protocol used in this study was adapted from a previous study by Mania et al [32]. After the trial, the zebrafish were fasted overnight and then suppressed.…”

Section: Zebrafish Feeding and Treatmentmentioning

confidence: 99%

Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice?

Imperatore

Tunisi

Mavaro

et al. 2020

Animals

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The 3,5-diiodo-L-thyronine (3,5-T2) is an endogenous metabolite of thyroid hormones, whose administration to rodents fed high-fat diet (HFD) prevents body weight increase and reverts the expression pattern of pro-inflammatory factors associated to HFD. The diet-induced obese (D.I.O.) zebrafish (Danio rerio) has been recently used as an experimental model to investigate fundamental processes underlying central and peripheral obesity-driven inflammation. Herein, we aim to understand the role of 3,5-T2 in regulating central and peripheral inflammation in D.I.O. model of zebrafish. 3,5-T2 (10 nM and 100 nM) was administered with the obesity-inducing diet (D.I.O. with 3,5-T2) or after 4 weeks of obesity-inducing diet (D.I.O. flw 3,5-T2). 3,5-T2 significantly increased the body weight and serum triglyceride levels in D.I.O. zebrafish in both conditions. Moreover, 3,5-T2 sustained or increased inflammation in the anterior (AI) and mid (MI) intestine when administered with the obesity-inducing diet, as indicated by the immunoexpression of the inflammatory markers tumor-necrosis factor-α (TNFα), cyclooxygenase 2 (COX2), calnexin, caspase 3, and proliferating cell nuclear antigen (PCNA). On the contrary, when 3,5-T2 was administered after the obesity-inducing diet, partly reverted the intestinal alteration induced by D.I.O. In addition, brain inflammation, as indicated by the increase in the activation of microglia, was detected in D.I.O. zebrafish and D.I.O. treated with 3,5-T2. These findings reveal that the effects of 3,5-T2 on fish intestine and brain can deviate from those shown in obese mammals, opening new avenues to the investigation of the potential impact of this thyroid metabolite in different diseases including obesity.

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“…Several data are present in the literature on the fish oral cavity morphology (for review, see Kapoor & Khanna, 1994), and several evidences are present regarding the strict connection among the different manners of prehension, oral transport, mixing and swallowing, the feeding habits and the oral cavity morphology. An important role by different hormones in feeding, related to the morphological characteristics, has been recently demonstrated in the digestive system in teleosts (Audira et al, 2018;Carnovali, Luzi, Terruzzi, Banfi, & Mariotti, 2018;Mania et al, 2017;Montalbano et al, 2016Montalbano et al, , 2018. The most of the functional processes inside the oral cavity are carried out by the tongue, whose presence and description have been showed in different species of fish, demonstrating the variation in the presence of anatomical structures like taste buds, mucous cells and teeth in teleosts, also of commercial interest (Abbate et al, 2006(Abbate et al, ,2017(Abbate et al, ,2020a(Abbate et al, ,2020bAbbate, Guerrera, Montalbano, De Carlos, et al, 2012;Alsafy, Bassuoni, & Hanafy, 2018;Amato et al, 2012;Dos Santos, Arantes, Santiago, & Dos Santos, 2015;El Bakary, 2014;Elgendy, Alsafy, & Tanekhy, 2016;Germanà et al, 2009;Guerrera et al, 2015;Ikpegbu, Ibe, & Nlebedum, 2019;Kasumyan, 2019;Kettratad, Senarat, Boonyoung, & Jiraungkoorsku, 2017;Levanti et al, 2017;Mahmoud, Essa, & Sayed, 2016;Sadeghinezhad, Rahmatiholasoo, Fayyaz, & Zargar, 2015;Sayed, Mahmoud, & Essa, 2019;Yu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Anatomical, histological and immunohistochemical study of the tongue in the rainbow trout (Oncorhynchus mykiss)

Abbate

Guerrera

Levanti

et al. 2020

Anat Histol Embryol

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The rainbow trout (Oncorhynchus mykiss Walbaum, 1792) is a fish commercially farmed all over the world. These fish are usually fed, in aquaculture, with pellets rich in proteins and fat. It is well known that there are close relationships among the adaptation of vertebrates to their environment, the capacity and the modality of feeding and the oral cavity morphology, especially the tongue one. No data are so far available about the morphology of the rainbow trout tongue, and therefore, the aim of this study was to investigate by light, scanning electron and confocal laser microscopy, the morphological characteristics of the tongue. An apex, a body and a root can be distinguished in the tongue, and the presence of teeth, taste buds and fungiformlike papillae was demonstrated. Light microscopy shows the presence of an adipose tissue pad in the deeper layer of the apex and in the most superficial layer of the root. In the deeper layer of the body, a triangular-shaped pad consisting of fusiform cells immersed in abundant extracellular matrix of the mesenchymal tissue was observed. The confocal laser microscopy shows the presence of cells with a fibroblast-like morphology positive for vimentin. In the deepest layer of the tongue root, a large area of osteo-cartilaginous tissue was observed. The results, besides the description of the morphological characteristics of the tongue, related to studies regarding the feeding, could be considered for the eventual applications of the use of mesenchymal cells, observed in adult fish, in cell therapies in different pathologies.

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Expression and distribution of leptin and its receptors in the digestive tract of DIO (diet-induced obese) zebrafish

Cited by 22 publications

References 69 publications

Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting

Presence and distribution of leptin and its receptor in the gut of adult zebrafish in response to feeding and fasting

Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice?

Anatomical, histological and immunohistochemical study of the tongue in the rainbow trout (Oncorhynchus mykiss)

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