Expression and distribution of glutathione transferases in protoscoleces of Echinococcus granulosus sensu lato

Biochemical characterization and gene structure analysis of the 24‐kDa glutathione transferase sigma from Taenia solium

“…Specific monoclonal antibodies against Ts24GST epitopes could also be used to classify helminth GST. The induction of the EgGST2 sigma gene by anthelminthic drugs suggests a role in drug resistance [26]. Moreover, rTs24GST has lower activity toward CDNB than rTs25GST and rTs26GST.…”

“…The gene architecture, splicing‐site conservation, distal and proximal elements found in the promoter, and similar molecular weight of the products that they encode suggest that Ts24gst is transcribed like mammalian GST genes are. Ts24gst also seems to be a housekeeping gene that can be induced by xenobiotics and drugs, as has been observed in other GST genes in different organisms [26,52]. rTs24GST does not bind to a GSH Sepharose‐4B column, so its properties such as its predicted pI and molecular weight allow for the design of methods for its purification in two steps.…”

“…The presence of sigma‐class GST in adult and cysticercus T. solium , especially in the scolex, suggests a potential role in several physiological processes. Such processes include detoxification of endogenous‐exogenous compounds and the regulation of host immune response, as reported in other organisms [ 26 , 44 , 45 ]. We characterized Ts24gst to test this possibility.…”

“…Recently, an omega class GST named EgrGSTO was reported in Echinococcus granulosus [ 25 ]. In cestodes, a sigma‐class GST has been associated with detoxification of H 2 O 2 , drug resistance, and an immunomodulation process, of which the latter is due to the property of prostaglandin D 2 synthase [ 26 , 27 ].…”

“…In T. solium , Ts24GST is abundant in the scolex and is associated with homeostasis processes related to detoxification [ 22 ]. Moreover, E. granulosus sigma‐class EgGST2 is associated with drug resistance and modulation of the host immune response [ 26 ]. In other helminths, such as Fasciola hepatica , sigma‐class GST has been identified in extracts of eggs and yolk cells and is associated with embryogenesis and reproduction.…”