Expression and distribution of connexin 40 in mammal heart

Gros, Daniël; Kempen, Marjan J. A. van; Théveniau, Magali; Delorme, Bruno; Jarry-Guichard, T.; Velde, I. Ten; Maro, Bernard; Briand, Jean‐Paul; Jongsma, Habo J.; Moorman, A. F. M.

doi:10.1016/b978-0-444-81929-1.50039-3

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…In addition, in the adult rat and mouse, CX43 is also absent from the ventricular conduction tissue, i.e., the His bundle and the proximal part of the bundle branches (Van Kempen et al, 1991;Gourdie et al, 1992). Interestingly, CX40 distribution studies (Gourdie et al, 1993;Gros et al, 1994Gros et al, , 1995Van Kempen et al, 1995) showed that CX40 has a more restricted distribution than CX43 in adult mammalian hearts. These authors demonstrated that (2x40 is expressed not only in atria (depending on the species) but also in ventricular conduction system myocytes.…”

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confidence: 99%

Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system

Delorme

Dahl

Jarry-Guichard

et al. 1995

Developmental Dynamics

150

108

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In adult mouse heart, CX40 is expressed in the atria and the proximal part of the ventricular conduction system (the His bundle and the upper parts of the bundle branches). This cardiac tissue is specialized in the conduction of the electrical impulse. CX40 is the only mouse connexin known to be expressed in these parts of the adult conductive tissue and is thus considered as a marker of the conduction system. In the present report, we investigated CX40 expression and distribution during mouse heart development. We first demonstrate that CX40 mRNA is regulated throughout development, as are other heart connexin transcripts, i.e., CX37, CX43, and (2x45, with a decreasing abundance as development proceeds. We also show that the CX40 transcript and protein are similarly regulated, CX40 being expressed as two different phosphorylated and un-phosphorylated forms of 41 and 40 kDa, respectively. Surprisingly, distribution studies demonstrated that CX40 is widely expressed in 11 days post-coitum (dpc) embryonic heart, where it is detected in both the atria and ventricle primordia. As development proceeds, the CX40 distribution pattern in the atria is maintained, whereas a more dynamic pattern is observed in the ventricles. From 14 dpc onwards, as the adult ventricular conduction system differentiates, CX40 decreases in the trabecular network and it is preferentially distributed in the ventricular conduction system. CX40 is thus the marker of the early differentiating conduction system. It is hypothesized that the conduction system is present in unorganized "embryonic" form at 11 dpc and trans-differentiates by 14 dpc into the adult conduction system. 0 1995 Wiley-Liss, Inc.

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mentioning

confidence: 99%

Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system

Delorme

Dahl

Jarry-Guichard

et al. 1995

Developmental Dynamics

150

108

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“…The presence of Cx in Capan-1 cells was investigated using specific antibodies directed against Cx37 [45], Cx40 [16], Cx43 [I31 and Cx45 [23] as well as two other anti-Cx antibodies (anti-Cx26 and anti-Cx32 ) purified as described by Dupont et a1 [9]. Immunofluorescence was carried out on: i) frozen sections of Capan-1 cell cultures and xenografts.…”

Section: Immunocytochemistry Of Gap Junctionsmentioning

confidence: 99%

Progressive development of gap junctions during growth of human pancreatic duct cells (Capan‐1) in vitro and in vivo

Monzat

Fanjul

Leclerc

et al. 1995

Biology of the Cell

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Among their numerous functions, gap junctions play a crucial role in proliferation, differentiation and secretion processes, although their existence and potential role in ion secretion in human pancreatic ducts have yet to be established. To investigate the morphogenesis and the role of gap junctions in human pancreatic duct cells, the Capan-1 cell line maintained in culture or heterotransplanted into nude mice was employed as model system. Capan-1 cells polarize during their growth in vivo and in vitro forming duct-like structures. Furthermore in culture, after confluence, these cells form domes, which is indicative of ion exchange processes. After treatment with tannic acid and freeze-fracture, gap junctions were observed along the basolateral membranes of Capan-1 cells on electron microscopic examination. The presence of alkaline phosphatases on gap junctions was demonstrated cytoenzymatically. In addition, cell-to-cell communication was visualized by microinjection of Lucifer yellow. During differentiation of Capan-1 cells in culture, the frequency of intercellular communications increased markedly over the period (days 11-13) when the cells form duct-like structures. The increase in gap junctions was demonstrated by analysis of the polarized cells organized in duct-like structures that are commonly observed in the tumors formed by heterotransplantation of Capan-1 cells into nude mice. Furthermore, gap junctions associated with tight junctions were also observed in the cells forming such structures. The role of gap junctions in ion exchange was evaluated by counting the number of domes in cultures treated with heptanol. Heptanol (an uncoupling agent of gap junction communication) completely inhibited dome formation in a reversible way, and reduced the frequency of intracellular communications by 44%. These results suggest that the gap junctions expressed by Capan-1 cells are involved in ion secretion by the human cancerous pancreatic duct cell line, Capan-1. In the present study, we show that: i) the expression of gap junctions is linked to development of the spatial conformation of the cells; and ii) gap junctions may be involved in ion secretion.

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Connexins in mammalian heart function

Gros

Jongsma²

1996

BioEssays

266

155

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In heart, the propagation of electrical activity is mediated by intercellular channels, referred to as junctional channels, aggregated into gap junctions and localised between myocytes. These channels consist of structurally related transmembrane proteins, the connexins, three of which (CX43, CX40 and CX45) have been shown to be associated with the myocytes of mammalian heart; a fourth, CX37, was detected exclusively in endothelial cells. In this paper, we review the recent data dealing with the topographical heterogeneity of expression of these connexins in the different cardiac tissues and the unique conductance properties of the channels they form, and attempt to assess the role played by each connexin and the consequences of their multiplicity in the propagation of action potentials.

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Expression and distribution of connexin 40 in mammal heart

Cited by 9 publications

References 18 publications

Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system

Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system

Progressive development of gap junctions during growth of human pancreatic duct cells (Capan‐1) in vitro and in vivo

Connexins in mammalian heart function

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