Expression and Diagnostic Value of miR-497 and miR-1246 in Hepatocellular Carcinoma

Abstract

ObjectiveSerum microRNAs (miRNAs) may serve as biomarkers in various cancers. Our study aims to explore the roles of miR-497 and miR-1246 in hepatocellular carcinoma (HCC).MethodsThe expression levels of miR-497 and miR-1246 were measured by RT-PCR. A correlation analysis was conducted between the expression levels of miR-497 and miR-1246 and clinicopathological characteristics of patients. The receiver operating characteristic (ROC) curve was applied to evaluate the diagnostic efficacy in HCC. In addition, bioinformatics tools were also utilized to predict the potential targets of miR-497 and miR-1246.ResultsThe expression level of miR-497 in HCC was significantly down-regulated compared with the control group while the miR-1246 revealed a significantly higher expression level in HCC. There was a significant correlation demonstrated between the expression levels of miR-497 and miR-1246 in preoperative serum of HCC and the differentiation degree, Tumor Node Metastasis (TNM) classification, and metastasis. The expression levels of serum miR-497 and miR-1246 were significantly associated with the diagnosis, prognosis, and overall survival rate of patients with HCC. Moreover, the potential target genes of miR-497 in HCC include ARL2, UBE2Q1, PHF19, APLN, CHEK1, CASK, SUCO, CCNE1, and KIF23. The low expression of these nine genes is associated with a better prognosis of HCC patients. AUTS2 is a novel target gene of miR-1246, and its low expression is significantly related to the low overall survival rate of HCC patients.ConclusionsmiR-497 and miR-1246 are possibly involved in the progression of HCC by regulating target genes, respectively, and could serve as biomarkers in HCC.

Exosomal microRNAs synergistically trigger stromal fibroblasts in breast cancer

“…In particular, its upregulation has been associated with tumor growth, metastasis, and drug resistance in different types of cancer. 72 , 73 , 74 In breast cancer, exosomal miR-1246 has been used as diagnostic biomarker because of its high expression specificity.…”

Decreased Expression of circ_0000160 in Breast Cancer With Axillary Lymph Node Metastasis

“…Recently, exome sequencing identified SUCO mutations in mesial temporal lobe epilepsy, and a lack of SUCO led to abnormal development of neurons ( Sha et al, 2015 ). SUCO was found to be upregulated in hepatocellular carcinoma ( Yue et al, 2019 ) and may be targeted by miR-497 ( Chen et al, 2021 ). Our data showed that circ_0000160 was downregulated, while the host gene SUCO showed no significant change in breast cancer with lymph node metastasis compared with those without lymph node metastasis.…”

Epigenetic modification-related mechanisms of hepatocellular carcinoma resistance to immune checkpoint inhibition

“…The expression of many microRNAs were closely associated with the degree of differentiation, and tumor metastasis of HCC, such as miR-497, miR-1246, and miR-378a-3p ( 135 , 136 ). In addition, miR-378a-3p was also proved to directly regulate PD-L1 and STAT3 signaling to inhibit HCC, which may be a potential target in the future ( 137 ).…”