Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana

Hakozaki, Hirokazu; Park, Jong In; Takada, Yukyo; Kazama, Tomohiko; Takeda, Y.; Suzuki, Go; Kawagishi-Kobayashi, Makiko; Watanabe, Masao

doi:10.1266/ggs.83.143

Cited by 17 publications

(9 citation statements)

References 34 publications

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“…In this study, we determined the full-length cDNA sequence of Eo KAS-II and Eg TKAS-II which are known for catalyzing the elongation of C 16:0 fatty acid-[ACP] to C 18:0 fatty acid-[ACP] in plastids. [13]…”

Section: Discussionmentioning

confidence: 99%

Insights from computational analysis of full-length β-ketoacyl-[ACP] synthase-II cDNA isolated from American and African oil palms

Bhore¹,

San

Amelia³

et al. 2014

J Nat Sc Biol Med

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Background:Palm oil derived from fruits (mesocarp) of African oil palm (Elaeis guineensis Jacq. Tenera) and American oil palm (E. oleifera) is important for food industry. Due to high yield, Elaeis guineensis (Tenera) is cultivated on commercial scale, though its oil contains high (~54%) level of saturated fatty acids. The rate-limiting activity of beta-ketoacyl-[ACP] synthase-II (KAS-II) is considered mainly responsible for the high (44%) level of palmitic acid (C16:0) in the oil obtained from E. guineensis.Objective:The objective of this study was to annotate KAS-II cDNA isolated from American and African oil palms.Materials and Methods:The full-length E. oleifera KAS-II (EoKAS-II) cDNA clone was isolated using random method of gene isolation. Whereas, the E. guineensis KAS-II (EgTKAS-II) cDNA was isolated using reverse transcriptase polymerase chain reaction (RT-PCR) technique; and missing ends were obtained by employing 5’and 3’ RACE technique.Results:The results show that EoKAS-II and EgTKAS-II open reading frames (ORFs) are of 1689 and 1721 bp in length, respectively. Further analysis of the both EoKAS-II and EgTKAS-II predicted protein illustrates that they contains conserved domains for ‘KAS-I and II’, ‘elongating’ condensing enzymes, ‘condensing enzymes super-family’, and ‘3-oxoacyl-[ACP] synthase II’. The predicted protein sequences shows 95% similarity with each other. Consecutively, the three active sites (Cys, His, and His) were identified in both proteins. However, difference in positions of two active Histidine (His) residues was noticed.Conclusion:These insights may serve as the foundation in understanding the variable activity of KAS-II in American and African oil palms; and cDNA clones could be useful in the genetic engineering of oil palms.

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Section: Discussionmentioning

confidence: 99%

Insights from computational analysis of full-length β-ketoacyl-[ACP] synthase-II cDNA isolated from American and African oil palms

Bhore¹,

San

Amelia³

et al. 2014

J Nat Sc Biol Med

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“…This protein is also involved in the regulation of fatty acid ratio during seed metabolism. It is required for embryo development, especially at the transition from globular to heart stage (Hakozaki H et al 2008). Another gene family responsible for membrane structure, the syntaxins (SYN) showed also unique expression in male tissues.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #1 of "Different expression pattern of flowering pathway genes contribute to male or female organ development during floral transition in the monoecious weed Ambrosia artemisiifolia L. (Asteraceae) (v0.2)"

Rocheta¹

2019

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The highly allergenic and invasive weed Ambrosia artemisiifolia L. (A. artemisiifolia) is a monoecius plant with separated male and female flowers. The genetic regulation of floral morphogenesis is a less understood field in the reproduction biology of this species. Therefore the objective of this work was to investigate the genetic control of sex determination during floral organogenesis. To this end we performed a genome-wide transcriptional profiling of vegetative and generative tissues during the plant development comparing wild-growing and in vitro cultivated plants. RNA-seq on Illumina NextSeq 500 platform with an integrative bioinformatics analysis indicated differences in 80 floral gene expressions depending on photoperiodic and endogenous initial signals. Sex specificity of genes was validated based on RT-qPCR experiments. We found 11 and 16 uniquely expressed genes in female and male transcriptomes that were responsible particularly to maintain fertility and against abiotic stress. High gene expression of homologous such as FD, FT, TFL1 and CAL, SOC1, AP1 were characteristic to male and female floral meristems during organogenesis. Homologues transcripts of LFY and FLC were not found in the investigated generative and vegetative tissues. The repression of AP1 by TFL1 homolog was demonstrated in male flowers resulting exclusive expression of AP2 and PI that controlled stamen and carpel formation in the generative phase. Alterations of male and female floral meristem differentiation were demonstrated under photoperiodic and hormonal condition changes by applying in vitro treatments.

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“…As a positive control, a gene encoding actin4 (accession no. NM001085300) was used, as described in Hakozaki et al (2008).…”

Section: Plant Materialsmentioning

confidence: 99%

Demonstration <i>in vivo</i> of the role of <i>Arabidopsis</i> PLIM2 actin-binding proteins during pollination

et al. 2013

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In plant reproduction, pollination is the initial key process in bringing together the male and female gametophytes. When a pollen grain lands on the surface of the stigma, information is exchanged between the pollen and stigmatic cell to determine whether the pollen grain will be accepted or rejected. If it is accepted, the stigmatic papilla cell supplies water and other resources to the pollen for germination and pollen tube elongation. Cellular processes involving actin are essential for pollen germination and tube growth, and actin-binding proteins regulate these processes by interacting with actin filaments to assemble cytoskeletal structures and actin networks. LIM proteins, which belong to a subfamily of cysteine-rich proteins, are a family of actin-binding proteins in plants, and are considered to be important for formation of the actin cytoskeleton and maintenance of its dynamics. Although the physiological and biochemical characteristics of LIMs have been elucidated in vitro in a variety of cell types, their exact role in pollen germination and pollen tube growth during pollination remained unclear. In this manuscript, we focus on the pollen-specific LIM proteins, AtPLIM2a and AtPLIM2c, and define their biological function during pollination in Arabidopsis thaliana. The atplim2a/atplim2c double knockdown RNAi plants showed a reduced pollen germination, approximately one-fifth of wild type, and slower pollen tube growth in the pistil, that is 80.4 μm/hr compared to 140.8 μm/hr in wild type. These defects led to an occasional unfertilized ovule at the bottom of the silique in RNAi plants. Our data provide direct evidence of the biological function of LIM proteins during pollination as actin-binding proteins, modulating cytoskeletal structures and actin networks, and their consequent importance in seed production.

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Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana

Cited by 17 publications

References 34 publications

Insights from computational analysis of full-length β-ketoacyl-[ACP] synthase-II cDNA isolated from American and African oil palms

Insights from computational analysis of full-length β-ketoacyl-[ACP] synthase-II cDNA isolated from American and African oil palms

Peer Review #1 of "Different expression pattern of flowering pathway genes contribute to male or female organ development during floral transition in the monoecious weed Ambrosia artemisiifolia L. (Asteraceae) (v0.2)"

Demonstration <i>in vivo</i> of the role of <i>Arabidopsis</i> PLIM2 actin-binding proteins during pollination

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