Expression and Cytogenetic Localization of the Human SM22 Gene (TAGLN)

Molecular analysis of an unstable genomic region at chromosome band 11q23 reveals a disruption of the gene encoding the α2 subunit of platelet-activating factor acetylhydrolase (Pafah1a2) in human lymphoma

“…We also showed that the gene SM22, encoding a protein speci®c for smooth muscle cells, was located just downstream of LPC in the opposite orientation. Our results correlate with the recently described cytogenetic localization and structure of the human gene (Camoretti-Mercado et al, 1998). Moreover, the position and the orientation of this gene are also conserved in the murine locus (Ebihara et al, submitted).…”

Hprt-targeted transgenes provide new insights into smooth muscle-restricted promoter activity

“…5). Although the hprt-targeted SM22␣ transgenes more closely recapitulate endogenous SM22␣ expression, which is expressed in all smooth muscle tissues (2,20,22), the lack of transgene expression in GI and reproductive tract smooth muscle cells suggests that additional, more distal, regulatory elements are required to drive SM22␣ expression in these tissues. In support of this proposal, a BAC clone encompassing the SM22␣ gene was expressed in all smooth muscle tissues in transgenic mice (33).…”

Cloning and Characterization of MST4, a Novel Ste20-like Kinase

“…The MST4 probe was a cDNA probe containing full-length MST4a. Fluorescence in situ hybridization was performed as described previously (38). Biotin-labeled probes were prepared by nick translation using Bio-16-dUTP (Enzo Diagnostics).…”