Expression and co-expression of surface markers of pluripotency on human amniotic cells cultured in different growth media

Bryzek, Aleksandra; Czekaj, Piotr; Plewka, Danuta; Komarska, H.; Tomsia, Marcin; Lesiak, Marta; Sieroń, Aleksander; Sikora, Jerzy; Kopaczka, Katarzyna

doi:10.17772/gp/1673

Cited by 21 publications

(13 citation statements)

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“…For example, the most stringent stem cell marker transcription factor, NANOG, is expressed on only 1–3% of hAESCs, while about 50% of hAESCs remain SSEA-4-positive at term [ 16 ]. Co-expression of stem cell surface markers SSEA-4, TRA1-60, and TRA1-81 is found on only about 4% of AE cells [ 37 ]. Some studies have confirmed that stem cell marker positive hAESCs are scattered randomly over the whole amnion membrane and appear to be surrounded by marker-negative cells, rather than existing in a cluster manner [ 26 , 38 , 39 ].…”

Section: The Plasticity Of Human Amniotic Epithelial Stem Cells Asmentioning

confidence: 99%

“…Moreover, another recent study demonstrated that hAESCs from different areas of amnion membrane (AM) have differences in pluripotency marker expression and proliferative ability [ 40 ]. These data demonstrate that the whole amniotic epithelium is covered with hAESCs at different development stages with diverse gene expression profiles and various degrees of stemness [ 37 ]. However, it is reported that most primary hAESCs, even those that are stem cell marker negative, can be induced towards specific differentiation in vitro [ 41 ].…”

Section: The Plasticity Of Human Amniotic Epithelial Stem Cells Asmentioning

confidence: 99%

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Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Qiu

Cui

et al. 2020

IJMS

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Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells (hAESCs) have obvious advantages and can be used as a novel potential cell source for cellular therapy and clinical application. hAESCs are known to possess stem-cell-like plasticity, immune-privilege, and paracrine properties. In addition, non-tumorigenicity and a lack of ethical concerns are two major advantages compared with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). All of the characteristics mentioned above and other additional advantages, including easy accessibility and a non-invasive application procedure, make hAESCs a potential ideal cell type for use in both research and regenerative medicine in the near future. This review article summarizes current knowledge on the characteristics, therapeutic potential, clinical advances and future challenges of hAESCs in detail.

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Section: The Plasticity Of Human Amniotic Epithelial Stem Cells Asmentioning

confidence: 99%

Section: The Plasticity Of Human Amniotic Epithelial Stem Cells Asmentioning

confidence: 99%

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Qiu

Cui

et al. 2020

IJMS

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“…Unlike ESCs and iPSCs, not all hAECs express these markers in a uniform manner, and even phenotypically stem cell marker‐negative hAECs maintain transcriptional stem cell‐like characteristics . Bryzek et al further investigated the co‐expressions of these stem cell surface markers and found only about 4% of cells co‐express SSEA‐4, TRA1‐60 and TRA1‐81 antigens. These data demonstrate that the term amniotic epithelium contains hAECs with various degrees of stemness, which is not surprising given that the pluripotent epiblasts begin differentiation into amnioblasts during formation of the amniotic cavity.…”

Section: Stem Cell Characteristics Of Amniotic Epithelial Cellsmentioning

confidence: 99%

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

Miki

2018

American J Rep Immunol

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Multiple stem cell types can be isolated from the human placenta. Recent advances in stem cell biology have revealed that human amniotic epithelial cells (hAECs) are one of the perinatal stem cells which possess embryonic stem cell-like differentiation capability and adult stem cell-like immunomodulatory properties. Unlike other types of placental stem cells, hAECs are derived from pluripotent epiblasts and maintain multilineage differentiation potential throughout gestation. Similar to mesenchymal stem cells, hAECs are also able to modulate the local immune response. These, and other properties, make hAECs attractive for cellular therapy. This review article summarizes current knowledge of stem cell characteristics and immunomodulatory properties of amniotic epithelial cells and aims to advance our understanding towards the goal of novel therapy development.

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“…On the contrary, markers routinely used to define and identify stromal cells are not detectable (4,5). The surface markers commonly observed on embryonic stem cells (e.g., globoseries glycolipids SSEA-3 and -4 and keratansulfateassociated Ags TRA 1-60 and 1-81) are consistently expressed, perhaps contributing to their multilineage differentiation potential (1,3,6). Unlike pluripotent cells, hAEC are not immortal; they do not express telomerase and maintain a normal karyotype, without resulting in tumor formation upon transplantation (3,7).…”

mentioning

confidence: 99%

Ectonucleotidase Expression on Human Amnion Epithelial Cells: Adenosinergic Pathways and Dichotomic Effects on Immune Effector Cell Populations

Morandi

Horenstein

Quarona

et al. 2019

The Journal of Immunology

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This study investigates the mechanism(s) underlying the immunoregulatory activities of placenta-derived human amnion epithelial cells (hAEC). The working hypothesis is that NAD + and ATP, along with ectoenzymes involved in their metabolism, play a significant role in hAEC-mediated immune regulation. Proof of principle of the hypothesis was obtained by analyzing the interactions between hAEC and the main human leukocyte populations. The results obtained indicate that hAEC constitutively express a unique combination of functional ectoenzymes, driving the production of adenosine (ADO) via canonical (CD39, CD73) and alternative (CD38, CD203a/PC-1, CD73) pathways. Further, the picture is completed by the observation that hAEC express A1, A2a, and A2b ADO receptors as well as ADO deaminase, the enzyme involved in ADO catabolism. The contribution of the purinergic mediator to immunomodulation was confirmed by exposing in vitro different immune effector cells to the action of primary hAECs. B cells showed an enhanced proliferation and diminished spontaneous apoptosis when in contact with hAEC. T cell proliferation was partially inhibited by hAEC through ADO production, as confirmed by using specific ectoenzyme inhibitors. Further, hAEC induced an expansion of both T and B regulatory cells. Last, hAEC inhibited NK cell proliferation. However, the involvement of ADO-producing ectoenzymes is less apparent in this context. In conclusion, hAEC exert different in vitro immunoregulatory effects, per se, as a result of interactions with different populations of immune effector cells. These results support the view that hAEC are instrumental for regenerative medicine as well as in therapeutic applications for immune-related diseases.

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Expression and co-expression of surface markers of pluripotency on human amniotic cells cultured in different growth media

Cited by 21 publications

References 0 publications

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications

Stem cell characteristics and the therapeutic potential of amniotic epithelial cells

Ectonucleotidase Expression on Human Amnion Epithelial Cells: Adenosinergic Pathways and Dichotomic Effects on Immune Effector Cell Populations

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