Expression and clinical significance of DNA‐Pk in nasopharyngeal carcinoma

Yan, Shan; Li, Liu; Xia, Yun; Liu, Zhi Gang; Zeng, Mu Sheng; Song, Li

doi:10.1016/j.cellbi.2008.01.161

Cited by 11 publications

(17 citation statements)

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“…CCT2 and CCT3) in oncogenesis and as prognostic factors have been confirmed in other instances of neoplasia such as acute myeloid leukaemia, 39 breast cancer, 40,41 small cell lung cancer, 42 gastric cancer, 43 colon cancer, 44,45 gallbladder adenocarcinoma 46 and hepatocellular carcinoma. 45,47 Similar to the afore-said genes, several studies have confirmed that the overexpression of PRKDC (DNA-dependent protein kinase catalytic subunit) is associated with tumour progression and tumour grade and is correlated with poor survival in breast cancer, 48,49 hepatocellular carcinoma, 50 nasopharyngeal carcinoma 51 and lung cancer. 52 In particular, various biological aspects of this gene have been thoroughly investigated in breast cancer and the findings have introduced it as a breast cancer cell growth facilitator, a prognostic predictor of survival and a predictive biomarker of response to adjuvant chemotherapy.…”

Section: Discussionmentioning

confidence: 89%

Development of an RNA sequencing‐based prognostic gene signature in multiple myeloma

2020

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Several prognostic gene signatures have been developed to predict the clinical outcome in patients with multiple myeloma (MM). The most salient disadvantage of the previous signatures is their non-reproducibility in external datasets. Given the disadvantages and the superiority of RNA sequencing over microarrays in transcriptome profiling to produce more reliable outputs, we sought to develop a reproducible RNA sequencingbased prognostic gene signature for MM. Genes significantly associated with survival were detected in The Cancer Genome Atlas (TCGA) MM RNA sequencing dataset (MMRF-CoMMpass) (n = 412) through a strict pipeline containing four rigid filters. The reproducibility of the selected genes was checked in an independent dataset (GSE24080), containing 559 newly diagnosed patients with MM. The RNA sequencing-based prognostic signature was reconstructed based on the final genes in the training dataset (MMRF-CoMMpass) and externally validated in five independent datasets (i.e. GSE2658, GSE13624, GSE9782, GSE6477 and GSE57317), containing 1461 MM cases. The RNA sequencing-based signature was reconstructed using finally five reproducible genes: CCT2, CKS1B, PRKDC, NONO and UBE2A. This signature was able to robustly discriminate between low-and high-risk patients in both training and validation datasets (Ps ≤ 0Á001). Our signature was also independent of and more powerful than the routine MM prognostic factors (i.e. b2-microglobulin, albumin, age and sex) (Ps ≤ 0Á01). Treatment regimens had no effect on RNA sequencing-based signature insofar as this signature succeeded in predicting the clinical outcome in various treatment groups (Ps ≤ 0Á001).

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Section: Discussionmentioning

confidence: 89%

Development of an RNA sequencing‐based prognostic gene signature in multiple myeloma

2020

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“…DSB is the main mechanism of tumor cell death after irradiation, and some protein involve in DSB could be used as a prognostic predictor for cancer (35). The major cause of radiotherapy failure is the success of DSB repair in tumor cells, leading to prolonged tumor cell survival.…”

Section: Discussionmentioning

confidence: 99%

Bmi-1 induces radioresistance in MCF-7 mammary carcinoma cells

Liu

et al. 2011

Oncology Reports

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Bmi-1, a member of the polycomb family, it is involved in self renewal of stem cells and functions as an oncogene in many malignant human cancer types. Recent studies have demonstrated that Bmi-1 is a predictive factor for poor patient prognosis. However, the underlying mechanisms of radioresistance mediated by Bmi-1 are poorly understood. In this study, the dose-survival relationship was analyzed using a clonogenic survival assay and combined radiation treatment with Bmi-1 overexpression or silencing. DNA double-strand break (DSB) and repair was assessed by immunofluorescence staining of γH2AX foci. In addition, mitochondrial membrane potential was detected between Bmi-1 knockdown and control MCF-7 cells after irradiation. Apoptosis and cell cycle were evaluated by flow cytometry. We found that exposure of MCF-7 cells overexpressing Bmi-1 to ionizing radiation resulted in dramatically enhanced survival relative to control cells, whereas cells with silenced Bmi-1 showed markedly reduced survival. Bmi-1 inhibition significantly increased DSBs and decreased DSB repair. Furthermore, Bmi-1 knockdown induced loss of mitochondrial membrane potential and enhanced apoptosis by up-regulating p53, p21, Bax expression and down-regulating p-AKT and Bcl-2 expression. These results indicate that Bmi-1 may play an important role in radiosensitivity, and the suppression of its expression might be a potential therapeutic target for breast cancer.

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“…MMP9 expression was identified to be increased in osteosarcoma, lung cancer, pancreatic cancer and CRC tissues to different extents, which was positively correlated with tumor metastasis (48)(49)(50). Similarly, it has been observed that MMP3 and MMP10 expression levels in lung cancer, esophageal cancer, liver cancer and endometrial adenocarcinoma tissues are higher than those in normal tissues, and that their expression has an association with tumor invasion, metastasis and proliferation (51)(52)(53)(54)(55). In addition, a review of a number of studies has demonstrated that MMP13 expression in malignant tumors, such as colon cancer, breast cancer, non-small cell lung cancer and oral squamous cell carcinoma, is closely associated with tumor occurrence, development, invasion and metastasis (56).…”

Section: Discussionmentioning

confidence: 99%

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

et al. 2017

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Abstract. Metastasis is one of the most aberrant behaviors of cancer cells. Patients with cancers, including colorectal cancer (CRC), have a higher risk of tumor recurrence and cancer-related mortality once metastasis is diagnosed. Existing treatment strategies fail to cure cancer mostly due to the onset of metastasis. Therefore, metastasis remains a challenge in cancer treatment. Some complementary and alternative medical therapies using traditional Chinese medicine have been demonstrated to be clinically effective in cancer treatment. Scutellaria barbata D. Don (SB) is a promising medicinal herb. It was previously reported that the ethanol extract of SB (EESB) is able to promote apoptosis, and inhibit cell proliferation and angiogenesis in human colon cancer cells. However, the anticancer effect of SB and the underlying mechanism require further investigation, particularly its role against metastasis. To further elucidate the antimetastatic effect of SB, MTT and Transwell assays were used in the present study to evaluate the effect of EESB on the proliferation, migration and invasion of the CRC cell line HCT-8. In addition, western blot analysis was performed to detect the expression of matrix metalloproteinases (MMPs), cadherins and other metastasis-associated proteins. EESB significantly reduced HCT-8 cell viability and attenuated the migration and invasion ability of HCT-8 cells in a dose-dependent manner. In addition, EESB decreased the expression of MMP-1, MMP-2, MMP-3/10, MMP-9 and MMP-13, and proteins in the phosphoinositide 3-kinase (PI3K)/AKT and transforming growth factor (TGF)-β/Smad pathways, but not the epithelial-mesenchymal transition (EMT)-related factors E-cadherin and N-cadherin. In conclusion, the results suggested that SB inhibits CRC cell metastasis via the suppression of PI3K/AKT and TGF-β/Smad signaling pathways, which may represent a mechanism by which SB exerts an anticancer effect.

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Expression and clinical significance of DNA‐Pk in nasopharyngeal carcinoma

Cited by 11 publications

References 0 publications

Development of an RNA sequencing‐based prognostic gene signature in multiple myeloma

Development of an RNA sequencing‐based prognostic gene signature in multiple myeloma

Bmi-1 induces radioresistance in MCF-7 mammary carcinoma cells

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

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