Expression and clinical significance of the microRNA-200 family in gastric cancer

Chang, Liang; Huo, Bingjie; Lv, Yalei; Wang, Yudong; Liu, Wei

doi:10.3892/ol.2015.3028

Cited by 36 publications

(38 citation statements)

References 23 publications

(22 reference statements)

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“…Therefore, it is of great significance to understand the molecular mechanism underlying GC carcinogenesis and development. In addition, an increasing number of studies have indicated that abnormal expression of miRNAs plays an important function in the initiation and progression of GC, and that miRNAs may be investigated as a potential novel therapeutic target for the treatment of GC (19,20). The present study revealed that miR-154 was significantly downregulated in GC tissues and cell lines.…”

Section: Discussionsupporting

confidence: 49%

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Qiao¹,

Cao²,

Yang³

2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a group of non-protein-coding, highly conserved single-stranded RNA molecules. The abnormal expression of miRNAs has been demonstrated to have an important function in the carcinogenesis and progression of gastric cancer. microRNA-154 (miR-154) has been reported to be downregulated in non-small cell lung, colorectal and prostate cancer. However, the expression and roles of miR-154 in gastric cancer remain to be established. The present study measured the expression levels of miR-154 in gastric cancer tissues and cell lines. miR-154 was found to be significantly downregulated in gastric cancer tissues and cell lines. In addition, functional studies indicated that the overexpression of miR-154 inhibited the proliferation, migration and invasion of gastric cancer cells. Using TargetScan, a dual luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis, metadherin (MTDH) was revealed as a novel miR-154 target. In addition, knocking down MTDH lead to a similar effect as overexpressing-154 in gastric cells. The present findings indicate that miR-154 was downregulated in gastric cancer, and inhibited tumor behaviors of gastric cancer cells partially through the downregulation of MTDH. Therefore, the miR-154/MTDH axis may be a novel therapeutic to treat patients with gastric cancer.

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Section: Discussionsupporting

confidence: 49%

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Qiao¹,

Cao²,

Yang³

2017

Oncology Letters

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“…miR-33a and miR-33b were found to globally regulate lipid metabolism by targeting numerous lipid metabolism-associated genes including abca1, crot, cpt1a, hadhb and ampk α [19]. The miR-200 family, including miR-200a, miR-200b, miR-200c, miR-141 and miR-429, has been reported to be dysregulated in several types of cancers including gastric cancer, breast cancer and bladder cancer [20–22]. In a previous study, we demonstrated that the reduction of miR-200a, miR-200b and miR-200c in hepatocytes contributed to IL-induced insulin resistance [23].…”

Section: Introductionmentioning

confidence: 99%

Reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription ofsrebp1

et al. 2016

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Previous studies indicated that miR-200s participated in IL-6-induced hepatic insulin resistance. However, the role of miR-200s in hepatic lipid accumulation has not been elucidated. Here we found that miR-200b and miR-200c were reduced in the steatotic livers of mice fed a high-fat diet (HFD) and patients with nonalcoholic fatty liver disease. This down-regulation was accompanied by an increase in the expression of lipogenic proteins such as sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS). The suppression of miR-200b and miR-200c in Hep1-6 and NCTC1469 hepatocytes enhanced intracellular triglyceride levels, which were associated with increased SREBP-1 and FAS protein levels. In contrast, the over-expression of miR-200b and miR-200c suppressed lipid accumulation and reduced the expression of SREBP1 and FAS in Hep1-6 and NCTC1469 cells transfected with miR-200b or miR-200c mimics. Importantly, the up-regulation of miR-200b and miR-200c could reverse oleic acid/palmitic acid-induced lipid accumulation in hepatocytes. A luciferase reporter assay identified that miR-200b and miR-200c could directly bind the 3′UTR of jun. JUN activated the transcription of srebp1 to increase lipid accumulation. The data also demonstrated that increased miR-200b and miR-200c expression might be associated with sitagliptin-reduced hepatic lipid accumulation in mice fed a HFD. These findings suggest, for the first time, that reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1.

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“…MIMAT0000071 (hsa-miR-17-3p) and MIMAT0000318 (hsa-miR-200b) are reported to be overexpressed in gastric cancer patients [54, 55]. MIMAT0000076 (hsa-miR-21) is used as potential diagnostic and prognostic biomarkers for gastric cancer [56], and MIMAT0004703 (hsa-miR-335-3p) is applied as prognostic signature in gastric cancer [57].…”

Section: Resultsmentioning

confidence: 99%

Computational Analysis of Specific MicroRNA Biomarkers for Noninvasive Early Cancer Detection

Song

Liang

Cao

et al. 2017

BioMed Research International

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Cancer is a complex disease residing in various tissues of human body, accompanied with many abnormalities and mutations in genomes, transcriptome, and epigenome. Early detection plays a crucial role in extending survival time of all major cancer types. Recent advances in microarray and sequencing techniques have given more support to identifying effective biomarkers for early detection of cancer. MicroRNAs (miRNAs) are more and more frequently used as candidates for biomarkers in cancer related studies due to their regulation of target gene expression. In this paper, the comparative analysis is used to discover miRNA expression patterns in cancer versus normal samples on early stage of eight prevalent cancer types. Our work focuses on the specific miRNAs biomarkers identification and function analysis. Several identified miRNA biomarkers in this paper are matched well with those reported in existing researches, and most of them could serve as potential candidate indicators for clinical early diagnosis applications.

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Expression and clinical significance of the microRNA-200 family in gastric cancer

Cited by 36 publications

References 23 publications

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription ofsrebp1

Computational Analysis of Specific MicroRNA Biomarkers for Noninvasive Early Cancer Detection

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