Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma

Abstract

Esophageal squamous cell carcinoma (ESCC) is a common gastrointestinal cancer with poor diagnosis due to metastasis. It has been found that Ezrin interacts with Ecadherin and plays a role in tumor cell metastasis. The present study detected the expressions of Ezrin and Ecadherin in ESCC using tissue array with immunohistochemistry, and analyzed their relations with malignancy degree and metastasis of ESCC, so as to provide new evidence for the diagnosis and prognosis prediction of ESCC.A tissue array consisting of 76 specimens of ESCC was prepared. The 76 patients, including 49 men and 27 women, underwent operation between July 2004 and July 2007 in Department of Thoracic Surgery of the Affiliated Hospital of Hebei University of Engineering. The median age was 62.5 years. None received radiotherapy or chemotherapy before operation. The diagnosis of ESCC was confirmed in all patients by postoperative pathologic examination. Of the 76 cases, 26 were well differentiated, 33 were moderately differentiated and 17 were poorly differentiated; 12 were at stage T1, 21 at stage T2, 29 at stage T3 and 14 at stage T4; 41 had lymphatic metastasis while 35 did not.Pathologic sections were prepared and HE staining was performed. One specimen of cancer tissue and one specimen of paracancer normal tissue (35 cm from the cancer tissue) were obtained from each case to prepare 10 mm 伊 8 mm arrays with a thickness of 34 滋 m using an arrayer (Beecher, USA). The tissue array with HE staining was examined by pathologists.Mouse antihuman Ezrin monoclonal antibody was produced by Neomarker Co., mouse antihuman Ecadherin monoclonal antibody by Santa Cruz Co., immunohistochemistry kit and diaminobenzidine (DAB) were purchased from Zymed Co. (USA). Immunohistochemical staining was performed according to the instructions of the kit. The sections were placed in an oven at 70益 overnight, dewaxed and hydrated, then put into 3% H 2 O 2 and incubated for 15 min to deactivate endogenous peroxidase, washed with PBS (pH 7.4) and put into citrate buffer (pH 6.0). Microwave or high pressure method was used to retrieve antigen for 15 min. The sections

Ezrin is Associated with Gastric Cancer Progression and Prognosis

“…Positive Ezrin expression was significantly higher in esophageal squamous cell carcinoma (ESCC) than in para-cancer normal squamous epithelium, and was related to invasiveness and lymph node metastasis of ESCC. The activation of Ezrin was shown to increase the tumorigenesis and metastasis of ESCC [7]. Elzagheid et al, showed that Ezrin expression was more intense in colon carcinomas than in rectal carcinomas, and may play a role in colorectal cancer progression.…”

“…Ezrin is found in many malignant cell types including primary gastrointestinal stromal tumors, esophageal squamous cell carcinoma, advanced colorectal cancer and prostatic carcinoma [6][7][8][9][10]. Bal et al, examined Ezrin expression in 75 gastric carcinoma (53 intestinal types of adenocarcinoma, 22 diffuse types of carcinoma) tissues using immunohistochemistry and found that Ezrin immunostaining was positive in 43 cases (81.1%) of intestinal-type and 9 (40.9%) cases of diffuse-type adenocarcinomas [11].…”

Knockdown of Ezrin by RNA Interference Reverses Malignant Behavior of Human Pancreatic Cancer Cells in Vitro

“…Suppression the over expression of Ezrin in rhabdomyosarcoma and osteosarcoma cells with highmetastatic potential could reduce their metastatic ability Yu et al, 2004). Not only that strong Ezrin immunoreactivity in tumor tissues correlate with a poor prognosis has been found in patients with so many malignancies, such as esophageal cancer (Chai et al, 2007;Zhai et al, 2010), gastric carcinoma (Shi et al, 2006), colorectal cancer (Elzagheid et al, 2008), astrocytic tumors (Geiger et al, 2000), highly malignant soft tissue sarcoma (Weng et al, 2005) and squamous cell carcinoma of the head and neck (Mhawech-Fauceglia et al, 2007). All these revealed Ezrin key role in malignant tumor cells migration and progression.…”

DRP-1, ezrin and E-cadherin expression and the association with esophageal squamous cell carcinoma

“…There were 22 and 47 ezrin-positive samples in the DRP-1-positive and -negative samples, respectively, indicating that there were significantly less ezrin samples in the DRP-1-positive samples than in the negative samples (P<0.05), indicating that the expression of the two genes was negatively correlated (Table III). In our previous study, a total of 16 and 43 positive samples were observed in the samples expressing normal or abnormal levels of E-cadherin, respectively, indicating that there was a decreased number of ezrin-positive samples in the tissues with normal E-cadherin expression than in those with abnormal expression, indicating that the two genes were negatively correlated (17). …”

“…Furthermore, its expression was found to be associated with the invasiveness of the cancer and lymph node metastasis, indicating that the expression of ezrin is an indication of tumor invasion and lymph node metastasis in patients, thereby resulting in a poor prognosis. Therefore, the detection of ezrin expression can be used to determine an early prognosis (17). In ovarian carcinoma, weak or absent ezrin expression in serous ovarian carcinoma has been associated with an unfavorable prognosis in patients (18).…”