Expression and clinical significance of lung‐specific X protein mRNA in bronchial brushing specimens from patients with or without lung cancer

Lv, Ming; Wu, Mingzhe; Zhao, Yujie; Yang, Dingquan; Wang, Enhua; Wu, Guang-Ping

doi:10.1111/j.1440-1843.2011.02008.x

Cited by 13 publications

(18 citation statements)

References 18 publications

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“…The authors concluded that using more sensitive nonsequencing methods could alleviate the need for LCM. Similarly, Lv et al [27] successfully extracted mRNA for the detection of LUNX (lung-specific X protein) by RT-PCR from bronchial brushes collected in SurePath fixative.…”

Section: Resultsmentioning

confidence: 99%

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

Michael

Bedrossian²

2014

Acta Cytologica

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Objective: First introduced for the processing of cervico-vaginal samples, liquid-based cytology (LBC) soon found application in nongynecological specimens, including bronchoscopic brushings, washings and transcutaneous and transbronchial aspiration biopsy of the lung as well as pleural effusions. This article reviews the existing literature related to these specimens along with the authors' own experience. Study Design: A literature review was conducted through Ovid MEDLINE and PubMed search engines using several key words. Results: Most of the literature is based on data collected through the use of split samples. The data confirms that the use of LBC is an acceptable, and sometimes superior, alternative to the conventional preparations (CP). LBC offers several advantages, including the ability to transport in a stable collecting media, elimination of obscuring elements, ease of screening, excellent preservation, random representative sample, and application of ancillary techniques on additional preparations. Some diagnostic pitfalls related to the introduced artifacts were reported. Conclusion: The utilization of LBC offers many advantages over CP and has a diagnostic accuracy that is equal to or surpasses that of CP. LBC affords a bridge to the future application of molecular and other ancillary techniques to cytology. Knowledge of the morphological artifacts is useful at the early stages of implementation.

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Section: Resultsmentioning

confidence: 99%

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

Michael

Bedrossian²

2014

Acta Cytologica

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“…This is the inverse of studies of mRNA expression of SPLUNC1 in the peripheral blood for the diagnosis of micrometastasis and the prognosis of lung cancer. (23,24) The reason may be related to the sensitivity of different methodology for protein and nucleic acid detection, though the higher protein levels of SPLUNC1 was determined in serum from lung cancer patients by our ELISA. A more sensitive method may need development for the detection from serum based on the MAbs.…”

Section: Discussionmentioning

confidence: 99%

“…(6) As an important addition to these, SPLUNC1 was also known as a potential marker for the detection of non-small lung cell cancer. (23,24) However, SPLUNC1 protein level investigations were lacking from NSCLC patients in the studies. In order to further explore the role of SPLUNC1 in lung cancer, a panel of specific MAbs was produced in our lab.…”

Section: Discussionmentioning

confidence: 99%

SPLUNC1 Is a Significant Marker in Pleural Effusion from Lung Cancer Compared to Tuberculosis

Xue

Yan

et al. 2015

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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SPLUNC1 (Short palate, lung and nasal epithelium clone1) protein is an abundant secretory product of epithelia present throughout the conducting airways. Although its function is still not fully known, most studies have focused on its defensive effect in the infection of human airways and its potential to serve as a molecular marker for lung cancer. In this study, we further evaluated the SPLUNC1 expression in patients with lung disease to explore its role in cancer or tuberculosis at the protein level. We generated a panel of antibodies by using protein from a eukaryotic expression system as the immunogen to mice. It was the panel of SPLUNC1 monoclonal antibodies that allowed us to comparatively determine SPLUNC1 protein in lung cancer and tuberculosis infection by detecting sera and pleural effusion other than airway surface. The results showed that the SPLUNC1 level was not significantly changed either from sera of lung cancer or control. There was a significant increase in pleural effusion from lung cancer when compared to tuberculosis. These results indicate that SPLUNC1 may be a useful marker for tracing lung cancer cells, based on its epithelial origin property in pleural effusion.

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“…RT-PCR can objectively detect even 1 cancer cell among 10 6 cells [18,19]. Identification of molecular markers for disease progression would be of great clinical value [20]. …”

Section: Discussionmentioning

confidence: 99%

Transcription Expression and Clinical Significance of mRNA of Vascular Endothelial Growth Factor and Endostatin in Liquid-Based Preparation Specimens from Patients with Cervical Dysplasia and Carcinoma

Zhang²,

Wu³

et al. 2013

Acta Cytologica

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Objectives: The aim of this study was to assess the diagnostic usefulness of vascular endothelial growth factor (VEGF) and endostatin mRNA in cervical specimens of patients with cervical dysplasia and carcinoma. Study Design: Transcription levels of VEGF and endostatin were detected by RT-PCR in cervical liquid-based preparation specimens and compared with cytological assessments. Results: VEGF as well as endostatin mRNA expression was significantly associated with either cytological or histological diagnosis (p < 0.05). VEGF mRNA and endostatin mRNA were significantly more likely to be expressed in squamous cell carcinomas (SCC) than in cervical intraepithelial neoplasia (CIN) III (p < 0.01 and p < 0.05), and obviously also more likely to be expressed in CINII than in CINI and in CINIII than in CINII (p < 0.05). Eleven inflammation lesions gave positive results by cytology but negative results by RT-PCR for VEGF and endostatin mRNA. Twenty-four SCC lesions gave false-negative or precancerous lesion results by cytology but positive results by RT-PCR for VEGF and/or endostatin mRNA expression. Conclusion: Transcription levels of VEGF and endostatin by RT-PCR may be an adjunct to cytology screening for early detection of cervical carcinomas and may determine the progressive potentiality of individual lesions, especially in high-risk patients.

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Expression and clinical significance of lung‐specific X protein mRNA in bronchial brushing specimens from patients with or without lung cancer

Cited by 13 publications

References 18 publications

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

SPLUNC1 Is a Significant Marker in Pleural Effusion from Lung Cancer Compared to Tuberculosis

Transcription Expression and Clinical Significance of mRNA of Vascular Endothelial Growth Factor and Endostatin in Liquid-Based Preparation Specimens from Patients with Cervical Dysplasia and Carcinoma

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